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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) ANNALES BOGORIENSES Jurnal Perikanan Universitas Gadjah Mada Jurnal Medik Veteriner Wahana Peternakan Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika

M Agus Setiadi

Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jl. Agathis, Kampus Dramaga IPB, Bogor, Jawa Barat, Indonesia 16880

Author-ID : 221524

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary

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Status of ram spermatozoa DNA after freeze-drying process Saili, Takdir; Prasetyaningtyas, wahono Esthi; Setiadi, Mohamad Agus; AgungPriyono, Srihadi; Boediono, Arief
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

The process of freeze drying caused detrimental effect on plasma membrane and acrosome of the spermatozoa, even it potentially could alter the chromatin and DNA integrities. On the other hand, DNA integrity is essential for spermatozoa to participate in pronucleus formation during fertilization event. Therefore the evaluation of DNA integrity should be carried out to study the effect of freeze drying process. EDTA, EGTA, and PBS were used as dilution media of spermatozoa prior to freeze drying process to protect the DNA. Toluidine blue staining and comet assay methods were used to evaluate the alteration on chromatin and DNA integrities of spermatozoa, respectively. The results revealed that the highest compacted chromatin after 6 months storage of freeze-dried spermatozoa were observed from EGTA-3 (98%) and EGTA-1 (97%) treatments that had significant differences compared to all PBS treatments (90-92%), but not for fresh spermatozoa (100%). Whereas, the highest compacted DNA integrity of freeze-dried spermatozoa were observed from EGTA-2 (92%) and EGTA-3 (92%) but had no significant differences compared to other treatments including fresh spermatozoa (97%). These results demonstrate that EDTA and EGTA tend to be able to protect chromatin and DNA integrities of ram spermatozoa during freeze-drying and storage compared to PBS. Key Words: Freeze-Drying, Spermatozoa, DNA, Toluidine Blue, Comet Assay

Biological Analysis of Leydig Cells-Conditioned Medium To Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Kaiin, Ekayanti Mulyawati; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Djuwita, Ita; Yusuf, Tuty Laswardi; Setiadi, Mohamad Agus
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v22i1.328

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro.Â LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50%Â LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured withÂ LCM has a positive reaction (57.4%) to histochemistry staining 3Î²-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCMÂ increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.

Sperm Preservation using Freeze-Drying Method TAKDIR SAILI; MULYOTO PANGESTU; MOHAMAD AGUS SETIADI; SRIHADI AGUNGPRIYONO; MOZES R. TOELIHERE; ARIEF BOEDIONO
HAYATI Journal of Biosciences Vol. 12 No. 1 (2005): March 2005
Publisher : Bogor Agricultural University, Indonesia

Since the discovery of cryopreservation method for bull semen, cryopreservation become an alternative method for maintaining gamet resources of certain animal which is threatened or near extinction. This technology was then applied to the preservation of embryo, oocyte, ovary and testis. The application of intracytoplasmic sperm injection (ICSI) for which sperm motility is unnecessary had supported the effort to create simplified method such as freeze-drying for sperm preservation. Due to the benefit of ICSI over the conventional in vitro fertilization (IVF) the spermatozoon could be mechanically driven to pass through the zona pellucida and entering the cytoplasm of oocytes prior to fertilization. The freeze-drying method is an alternative method in sperm preservation which ignored the motility of sperm. The sperm resulted from this technique is in drying state, therefore, it might be stored in room temperature or in refrigerator. Many reports have claimed that freeze-dried sperm which is not motile but has an intact DNA was able to fertilize oocytes, even produced offspring in mouse.

Maturation Rate of Ovine Oocytes from Different Reproductive Status and Maturation Medium ARIEF BOEDIONO; YULNAWATI YULNAWATI; MOHAMAD AGUS SETIADI
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

The aim of the present study was to determine the number of follicles, oocyte quality and maturation rate of oocytes from pairs of ovary with different reproductive status in two maturation medium, TCM-199 as control and CR1aa as treatment. The pairs of ovary were classified into four groups: (i) ovaries with corpus luteum (CL) and dominant follicle (DF), (ii) ovaries with CL, without DF, (iii) ovaries with DF, without CL, (iv) ovaries without both CL and DF. Results of the experiment revealed that the greatest number of follicles was observed from ovary with CL without DF (15.88 + 10.68), although not significantly different (P > 0.05) with other status of ovaries. The lowest number (P < 0.05) of A grade oocytes was found from ovary with DF without CL (1.20 + 1.10). The percentage of Metaphase II was highest in TCM-199 (75.51%) with oocytes from ovaries with CL and DF, and the lowest with oocytes from ovaries with DF without CL in TCM-199 and CR1aa (42.86 and 30.95%). The study suggested that the number of oocytes with A grade were influenced by the reproductive status of ovaries. The maturation rate of A grade oocytes was influenced by the quality of oocytes and the composition of maturation medium. Key words: reproductive status, corpus luteum, dominant follicles, TCM-199, CR1aa

Cysteamine in Maturation Medium Enhances Nuclear Maturation and Fertilization Rate of Sheep Oocytes In Vitro Feni Dwi Kartika Gulo; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
HAYATI Journal of Biosciences Vol. 27 No. 4 (2020): October 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.4.290

Low nuclear maturation and fertilization rate is one obstacle in the in vitro embryo production which decrease embryo yield. This problem is presumable related with high production of reactive oxygen species (ROS) during maturation process. Glutathione (GSH) as an antioxidant is well known to overcome effect of ROS production. GSH synthesis in the cytosol part of the oocyte cytoplasm is influenced by cysteine availability. It is therefore, this research was conducted to evaluate the ability of cysteamine to provide cysteine availability as GSH precursor on the nuclear maturation and fertilization rate of sheep oocytes. Results of this experiment revealed additional cysteamine at 150 µm and 200 µm could significantly improve nuclear maturation rate. On the other side, although additional of cysteamine at 50 µm could not improve nuclear maturation rate, however 50 µm cysteamine in the maturation medium could significantly improve the fertilization rate. Based on those experiment results, it seems that the additional cysteamine might be improve not only GSH availability but also the oocyte quality which characterized the ability of pronuclear formation. This finding strongly suggested that additional cysteamine in the maturation medium could improve nuclear maturation and fertilization rate of sheep oocytes.

Ovarian Dynamic in Ongole Grade Cattle after GnRH Injection in Ovsynch Protocol Based on Progesterone Device Muhammad Imron; Iman Supriatna; . Amrozi; Muhammad Agus Setiadi
Media Peternakan Vol. 38 No. 2 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

PO cattle have weaknesses to show clear estrus signs which cause difficulty in artificial insemination implementation. The present study was designed to obtain ovarian dynamic as effect of GnRH injection in ovsynch protocol based on progesterone intravaginal device. Heifers (18) and cows (n= 12) were allocated to one of three groups. Cuemate-PGF2α (CP) group inserted with a Cuemate on day 0-7 and injected with prostaglandin on day 7. Cuemate-PGF2α-GnRH (CPG) group was treated as CP group with the addition of GnRH injection on day 9. GnRH-Cuemate-PGF2α-GnRH (GCPG) group was treated as CPG group with addition of GnRH injection on day 0. Ultrasonography was performed on days 0-3, day 7 until ovulation and 7 days after ovulation. Percentage of ovulation synchronization increased significantly (P<0.01) between CP, CPG, and GCPG, respectively, both in heifers (16%, 50%, and 85%, respectively) and cows (0%, 60%, and 100%, respectively), on day 11. Preovulatory follicle diameters between CP, CPG, and GCPG treatments were not different significantly both in heifers (11.9±0.5, 11.9±0.5, and 12.1±0.6 mm, respectively) and cows (11.7±0.4, 11.8±0.7, and 11.1±0.6 mm, respectively). This study concluded that GCPG protocol increased the synchrony of ovulation rate both in cows and heifers, without affecting the follicle preovulatory and CL diameters.Key words: ovarian dynamic, GnRH, ovsych, PO Cattle

Koleksi Sel Telur dengan Teknik Laparoskopi untuk Produksi Embrio dan Transfer Embrio pada Domba Mohamad Agus Setiadi; Iman Supriatna; Arief Boediono
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.17 KB)

An experiment was carried out to analyze the application of laparoscopic technique for oocyte collection, in vitro embryo production and embryo transfer in sheep. The first experiment was conducted to observe effect of gonadotropin stimulation on follicle development and laparoscopic technique for oocytes aspiration. In the second experiment, effect of culture system on the embryo development in vitro was assessed and in the third experiment, the application of laparoscopic for embryo transfer has been conducted. The result showed that single dose of gonadotrophin was sufficient to support follicle development significantly and it could help follicle visualization. It also showed that laparoscopic ovum-pick up could be conducted weekly without any restriction The second series experiment showed CR1aa culture system was better than TCM 199 (29.90°/o vs 8.00%) and the changing of media was required to ensure better metabolism process for embryos. The third experiment revealed that embryo transfer could be conducted with an aid from laparoscope. In conclusion, single dose PMSG stimulation is sufficient to support follicle development for /aparoscopic ovum-pick up, the culture media changing affects the successful rate of in vitro embryo production (8% vs 25.66%) and the laparoscopy technique can be used safely for embryo transfer on sheep.Keyword: laparoscopic, oocyte, embryo transfer, sheep

Hubungan Temperatur, Kelembaban, dan Manajemen Pemeliharaan terhadap Efisiensi Reproduksi Sapi Perah di Kabupaten Bogor Dadang Jaenudin; Akhmad Arif Amin; Mohamad Agus Setiadi; Hadi Sumarno; Sri Rahayu
Acta VETERINARIA Indonesiana Vol. 6 No. 1 (2018): Januari 2018
Publisher : IPB University

Penelitian ini bertujuan untuk mengamati dan mengkaji hubungan suhu, kelembaban dan manajemen ternak terhadap efisiensi reproduksi sapi perah di Kabupaten Bogor. Suhu dan kelembaban udara diamati pagi, siang dan sore hari. Data suhu dan kelembaban udara dikonversi ke nilai indeks suhu kelembaban udara (THI). Pengamatan parameter efisiensi reproduksi dilakukan dengan menghitung nilai Days Open (DO), Conception Rate (CR), Service per Conception (S/C) dan First Service. Hasil penelitian menunjukkan bahwa nilai THI Acep Farm Kunak (77,21±0,71) lebih tinggi (P<0,05) dari Cifa Farm (71,27±0,50) dan Erif Farm (71,22±0,41). Days Open Acep Farm Kunak (110,76±37,34 hari) lebih panjang (P<0,05) dibandingkan dengan Cifa Farm (88.39±23,80 hari) dan Erif Farm (88,20±22,39 hari). Nilai CR Acep Farm (75,76±0,11%) dan Cifa Farm (66,13±0,10%) tidak berbeda (p>0,05) dibandingkan dengan Erif Farm (42,00±0,08%). Nilai S/C di tiga peternakan tidak menunjukkan perbedaan (p>0,05) masing-masing nilai S/C Cifa Farm (1,42±0,64), Erif Farm (1,52±0,64) dan Acep Farm Kunak (1,64±0,82). First Service Cifa Farm (76,11±13,84) dan Erif Farm (75,17±13,17) berbeda (P<0,05) dengan Acep Farm Kunak (96,42±35,49). Dapat disimpulkan bahwa suhu, kelembaban udara dan manajemen pemeliharaan dapat memengaruhi DO sapi perah di ke tiga peternakan sapi perah di ke tiga lokasi penelitian. Lebih lanjut, diindikasikan THI memengaruhi zona nyaman peternakan sapi perah, namun tidak ditemukan korelasi positif antara nilai THI dengan tingkat efisiensi reproduksi.

Daya Fertilisasi Spermatozoa Kauda Epididimis Domba dengan atau tanpa Swim Up sebelum Fertilisasi Nur’aisyah Amrah Safitri; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi; Mokhamad Fahrudin
Acta VETERINARIA Indonesiana Vol. 5 No. 1 (2017): Januari 2017
Publisher : IPB University

Penelitian ini dilakukan untuk mengevaluasi penggunaan metode swim up untuk persiapan spermatozoa sebelum fertilisasi terhadap tingkat fertilisasi in vitro spermatozoa kauda epididimis pasca penyimpanan selama 48 jam. Kauda epididimis domba disimpan pada suhu 4 oC selama 0 hari (H-0), 1 hari (H-1) dan 2 hari (H-2), kemudian spermatozoa dikoleksi dan dibekukan. Spermatozoa ejakulat beku digunakan sebagai kontrol. Oosit yang telah matang difertilisasi secara in vitro dengan spermatozoa asal kauda epididimis pasca penyimpanan dan ejakulat menggunakan metode persiapan spermatozoa dengan dan tanpa swim up. Hasil penelitian menunjukkan bahwa spermatozoa asal kauda epididimis yang dikoleksi segera setelah kematian hewan (H-0) memiliki kemampuan yang sama dengan spermatozoa ejakulat (P>0,05). Tingkat fertilisasi spermatozoa kauda epididimis pasca penyimpanan selama 2 hari mengalami penurunan seiring bertambahnya waktu simpan. Penggunaan metode swim up dan tanpa swim up menunjukkan kemampuan fertilisasi yang sama pada spermatozoa ejakulat dan spermatozoa kauda epididimis yang disimpan. Dapat disimpulkan bahwa metode swim up tidak berpengaruh terhadap tingkat fertilisasi in vitro spermatozoa asal kauda epididimis yang disimpan pada suhu 4 °C selama 2 hari. Kemampuan fertilisasi spermatozoa asal kauda epididimis domba yang disimpan pada suhu 4 °C mengalami penurunan sampai hari kedua, namun spermatozoa tersebut masih mampu membuahi oosit secara in vitro.

Respons dan Karakteristik Estrus setelah Sinkronisasi Estrus dengan Cloprostenol pada Sapi Friesian Holstein Musthamin Balumbi; Iman Supriatna; Mohamad Agus Setiadi
Acta VETERINARIA Indonesiana Vol. 7 No. 1 (2019): Januari 2019
Publisher : IPB University

Penelitian tentang sinkronisasi estrus pada sapi perah Friesian Holstein (FH) menggunakan cloprostenol telah dilakukan. Penelitian ini bertujuan untuk mengamati respons dan karakteristik estrus serta pengukuran nilai hambatan arus listrik lendir vagina. Penelitian dilakukan pada 20 ekor sapi FH dengan dua perlakuan injeksi cloprostenol, yaitu injeksi tunggal dengan keberadaan corpus luteum dan injeksi ganda dengan selang waktu injeksi 11 hari. Pengamatan estrus dan pengukuran lendir dilakukan lima hari berturut-turut setelah injeksi cloprostenol yang terakhir. Parameter pengukuran yang diamati meliputi respons, onset, dan durasi estrus, serta nilai hambatan arus listrik lendir vagina. Hasil penelitian menunjukkan bahwa injeksi ganda menghasilkan respons estrus yang lebih baik dibandingkan injeksi tunggal (90% vs 70%), dengan onset estrus lebih pendek (47,55 jam vs 53,28 jam), dan durasi estrus lebih pendek (24,85 jam vs 23,55 jam). Data hambatan arus listrik lendir vagina pada kelompok injeksi ganda lebih rendah dibandingkan dengan injeksi tunggal (187,77 vs 192,14), dengan pola tinggi sebelum estrus, rendah pada saat estrus, dan tinggi kembali setelah estrus, baik pada kelompok injeksi tunggal maupun injeksi ganda. Dapat disimpulkan bahwa respons estrus pada kelompok injeksi ganda lebih baik dibandingkan dengan kelompok injeksi tunggal.

Co-Authors . Hasbi Achmad Setiyono Adnin Adnan Agus Setiadi Agus Setiyono Alvien Nur Aini Amrozi Anak Agung Istri Sri Wiadnyani Ananda Ananda Anita Hafid Aras Prasetiyo Nugroho Arie Febretrisiana Arief Boediono Aries Boediono Ario Damar Asep Kurnia Asnath Maria Fuah, Asnath Maria Bambang Purwantara Bayu Rosadi Boenjamin Setiawan Dadang Jaenudin Dondin Sajuthi Ekayanti M. Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Muyawati Kaiin Evy Damayanthi Faisal Amri Satrio Feni Dwi Kartika Gulo Frilianty Putri Frilianty Putri Gustina, Sri Hadi Sumarno Hafizuddin Hafizuddin Harry Murti Hasbi . Hasbi Hasbi Hasim Heri Sujoko I Ketut Mudite Adnyana Idqan Fahmi Iman Supriatna IPB, DGB Ita Djuwita Ita Djuwita ITA DJUWITA Ita Djuwita Kaiin, Ekayanti Mulyawati Ketut Adnyane Mudite Kusdiantoro Mohamad La Ode Muhammad Aswad Salam Lala M Kolopaking Lisa Dwi Fannessia Lisa Praharani Luki Abdullah M Agil M Noordin M. Haviz M. Khoeron . Ma'mun Sarma Masir, Ummul Masturi Muhajir Mitha Kurnia Sari Mohamad Fahrudin Mokhamad Fahrudin MOZES R. TOELIHERE MUHAMMAD AGIL Muhammad Imron Muhammad Imron Muhammad Imron MULYOTO PANGESTU Musthamin Balumbi Nahrowi Neta Fitria Yasa Ni Wayan Kurniani Karja Nofri Zayani Novi Suprihatin Nurbety Tarigan Nurkarimah, Dona Astari Nur’aisyah Amrah Safitri Okky Adi Bintara Oktariza, Wawan Praharani, Lisa Purwiyatno Hariyadi Rachmat Herman Rahmatullah Rahmatullah Reski Adelia Ridwan Affandi Rimas Prathita Agustin Rimayanti - Rizky Amrullah Chaniago Ronny Rachman Noor Satya Gunawan Somanjaya, Rachmat Soni Sopiyana Sri Gustina Sri Purwaningsih, Sri SRI RAHAYU Sri Rahayu Srihadi Agungpriyono Sudradjat Sumiati Suria Darma Tarigan Syafri Nanda Syahruddin Said TAKDIR SAILI Takdir Syahruddin Said Teguh Sumarsono Tuty L. Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Tuty Laswardi Yusuf Ulfah Juniarti Siregar Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Widyasanti, Ni Wayan Helpina YULNAWATI YULNAWATI Zultinur Muttaqin

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