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All Journal Indonesian Journal of Herbal and Biomedical Research
Puspa Amalia
Program Studi D4 Teknologi Laboratorium Medis, Politeknik ‘Aisyiyah Pontianak

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Uji Aktivitas Ekstrak Etanol Kapulaga (Amomum compactum) sebagai Penghambat Pertumbuhan Candida albicans Marda Hamelya Putri; Khoirul Rista Abidin; Khairul Bariyah; Puspa Amalia
Indonesian Journal of Herbal and Biomedical Research Vol. 1 No. 1 (2025)
Publisher : Bahiraliya Natura Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.66982/ijhbr.v1i1.2

Abstract

This study aimed to evaluate the antifungal effectiveness of ethanol extract of cardamom against Candida albicans. The well diffusion method was used with extract concentrations of 5%, 10%, and 15%, alongside a positive control (ketoconazole). The inhibition zone was measured to assess antifungal activity. The 15% extract concentration produced an average inhibition zone of 16.56 ± 0.86 mm, categorized as moderate inhibition. These findings suggest that A. compactum possesses antifungal potential due to its bioactive compounds, such as flavonoids and tannins, which are known for their antimicrobial properties. The growing resistance of Candida albicans to synthetic antifungal agents highlights the importance of exploring natural alternatives. Hence, A. compactum extract demonstrates promising potential as a natural antifungal candidate that could be further developed for pharmaceutical or herbal formulations. The 15% ethanol extract of A. compactum exhibited measurable inhibitory activity against Candida albicans, with inhibition zones of 16.7 mm, 17.65 mm, 16.3 mm, and 15.6 mm across four replications. The mean inhibition zone was 16.56 mm with a standard deviation of 0.86 mm, indicating moderate antifungal strength. The variation among replicates was within the expected range for microbiological assays and may result from minor differences in extract diffusion or fungal growth conditions.
Evaluasi Efektivitas Daun Bayam Merah (Amaranthus tricolor L.) Berdasarkan Waktu Perendaman sebagai Pewarna Alternatif pada Pemeriksaan Escherichia coli Kalisti Meriani Papalanggi; Fath Dwisari; Puspa Amalia
Indonesian Journal of Herbal and Biomedical Research Vol. 1 No. 1 (2025)
Publisher : Bahiraliya Natura Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.66982/ijhbr.v1i1.4

Abstract

Escherichia coli is one of the Gram negative bacteria and the main bacteria that causes diarrhea. The identification of E. coli can be used by Gram staining using safranin. However, safranin has several limitations, which are expensive and carcinogenic. Therefore, an alternative dyes based on natural compounds such as red spinach leaves are needed. The purpose of this study was to determine the potential of soaking time variations of red spinach leaves (Amaranthus tricolor L) as an alternative dye and the effect of soaking time variations on the examination of Escherichia coli staining. This research was conducted by quasi-experiments, using purposive sampling with a total of 27 samples and safranin as positif control. The Fisher's exact statistical test obtained p-value<0.05 which stated there were difference between red spinach leaves and safranin in staining of E. coli bacteria. The present study exhibited the potency of (Amaranthus tricolor L.) as an alternative dye for E. coli staining, but had not shown better results compared to safranin.
Co-Authors Fath Dwisari Kalisti Meriani Papalanggi Khairul Bariyah Khoirul Rista Abidin Marda Hamelya Putri