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All Journal Indonesian Journal of Herbal and Biomedical Research
Fath Dwisari
Program Studi D4 Teknologi Laboratorium Medis, Politeknik ‘Aisyiyah Pontianak

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Evaluasi Efektivitas Daun Bayam Merah (Amaranthus tricolor L.) Berdasarkan Waktu Perendaman sebagai Pewarna Alternatif pada Pemeriksaan Escherichia coli Kalisti Meriani Papalanggi; Fath Dwisari; Puspa Amalia
Indonesian Journal of Herbal and Biomedical Research Vol. 1 No. 1 (2025)
Publisher : Bahiraliya Natura Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.66982/ijhbr.v1i1.4

Abstract

Escherichia coli is one of the Gram negative bacteria and the main bacteria that causes diarrhea. The identification of E. coli can be used by Gram staining using safranin. However, safranin has several limitations, which are expensive and carcinogenic. Therefore, an alternative dyes based on natural compounds such as red spinach leaves are needed. The purpose of this study was to determine the potential of soaking time variations of red spinach leaves (Amaranthus tricolor L) as an alternative dye and the effect of soaking time variations on the examination of Escherichia coli staining. This research was conducted by quasi-experiments, using purposive sampling with a total of 27 samples and safranin as positif control. The Fisher's exact statistical test obtained p-value<0.05 which stated there were difference between red spinach leaves and safranin in staining of E. coli bacteria. The present study exhibited the potency of (Amaranthus tricolor L.) as an alternative dye for E. coli staining, but had not shown better results compared to safranin.
Analisis Pengaruh Waktu Fermentasi terhadap Konsentrasi Etanol pada Kulit Pisang Raja (Musa acuminata) dan Pisang Kepok (Musa paradisiaca L) Rahmadian Hoirunnisa; Khoirul Rista Abidin; Fath Dwisari; Abdurraafi' Maududi Dermawan
Indonesian Journal of Herbal and Biomedical Research Vol. 1 No. 1 (2025)
Publisher : Bahiraliya Natura Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.66982/ijhbr.v1i1.5

Abstract

Banana peel waste is frequently discarded without proper management, potentially causing long-term environmental problems. However, banana peels can be valorized as a renewable feedstock for ethanol production. This study employed a quantitative experimental design consisting of four main stages: sample preparation, sulfuric acid hydrolysis, yeast fermentation with fermentation time variations of 72, 120, and 168 h, and ethanol content analysis. The ethanol content obtained from Raja banana (Musa acuminata) peels at fermentation times of 72, 120, and 168 h was 15%, 16%, and 17%, respectively. Meanwhile, ethanol yields from Kepok banana (Musa paradisiaca L.) peels at the same fermentation durations were 17.66%, 20.33%, and 22.33%, respectively. The results indicated that the highest ethanol content for both Raja and Kepok banana peels was achieved at a fermentation time of 168 h. Friedman test analysis revealed significance values of 0.368 (> 0.05) for Raja banana peels and 0.097 (> 0.05) for Kepok banana peels, indicating no statistically significant differences in ethanol content among the three fermentation durations. Overall, an increasing trend in ethanol content was observed for both banana varieties with prolonged fermentation time; however, statistical analysis confirmed that the differences were not significant across the tested fermentation periods.
Co-Authors Abdurraafi' Maududi Dermawan Kalisti Meriani Papalanggi Khoirul Rista Abidin Puspa Amalia Rahmadian Hoirunnisa