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All Journal The Indonesian Biomedical Journal Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Jurnal Kedokteran Meditek Indonesian Journal of Pharmacology and Therapy
Moehario, Lucky Hartati
Departemen Mikrobiologi, Fakultas Kedokteran Dan Ilmu Kesehatan, UNIKA Atma Jaya, Jakarta
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Gene Families of AmpC-producing Enterobacteriaceae Present in the Intensive Care Unit of Cipto Mangunkusumo Hospital Jakarta Lucky Hartati Moehario; Thomas Robertus; Anis Karuniawati; Rudyanto Sedono; Delly Chipta Lestari; Andi Yasmon
The Indonesian Biomedical Journal Vol 11, No 1 (2019)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v11i1.552

Abstract

BACKGROUND: Antibiotic resistance has become a worldwide problem. Among Asia countries, Indonesia has high prevalence of multi-drug resistant organisms mainly due to Gram-negative bacilli Enterobacteriaceae. This study aimed to find out whether gene family of AmpC and AmpC/ESBL-producing Enterobacteriaceae were present in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital, Jakarta, Indonesia.METHODS: Specimens were obtained from several body sites of adult patients with infection hospitalised in ICU of Cipto Mangunkusumo Hospital. VITEK®2 was used to identify the microorganisms. Antibiotic susceptibility tests were conducted using VITEK®2 and disc diffusion technique according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Double disc synergy (DDS) test method was employed to detect AmpC activity. Gene families of ampC were identified using multiplex polymerase chain reaction (PCR).RESULTS: Forty five isolates were identified as putative AmpC, extended-Spectrum β-lactamases (ESBL) and AmpC/ESBL-producing Enterobacteriaceae. Klebsiella pneumoniae (n=32) were predominant, followed by Escherichia coli (n=6), Enterobacter cloacae (n=5) and Enterobacter aerogenes (n=2). AmpC activity was detected in 9 isolates, in which 4 isolates were AmpC producing and 5 isolates were AmpC/ESBL. In vitro, AmpC-producing Enterobacteriaceae showed good susceptibility to many antibiotic tested, while those of AmpC/ESBL-producing only to Amikacin. The gene families of ampC were DHA, EBC and CIT identified from 6 isolates.CONCLUSION: DHA, EBC and CIT gene families were identified from AmpC and AmpC/ESBL-producing Enterobacteriaceae in the ICU of Cipto Mangunkusumo Hospital. While the AmpC-producing was still susceptible to almost all antibiotics tested, the AmpC/ESBL-producing showed resistant except for Amikacin.KEYWORDS: Enterobacteriaceae, β-lactamases, AmpC, ESBL
Aktivitas Antibakteri Ekstrak Kelopak Bunga Rosela terhadap Bakteri Multidrug Resistant Acinetobacter baumannii dan Pseudomonas aeruginosa Elisha Minarto; Irene; Lucky Hartati Moehario
Jurnal Kedokteran Meditek Vol 28 No 3 (2022): SEPTEMBER-DESEMBER
Publisher : Fakultas Kedokteran Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/jkdoktmeditek.v28i3.2366

Abstract

Penggunaan berbagai macam antibiotik menyebabkan kejadian resistensi antibiotik cepat berkembang. Acinetobacter baumannii dan Pseudomonas aeruginosa adalah bakteri multidrug resisten yang sering dikaitkan dengan terjadinya kegagalan terapi. Berbagai negara menggunakan herbal sebagai pengobatan alternatif penyakit infeksi. Studi sebelumnya menunjukkan aktivitas antibakteri yang dimiliki Hibiscus sabdariffa (rosela). Penelitian ini bertujuan untuk meneliti aktivitas antibakteri ekstrak kelopak bunga rosela pada berbagai konsentrasi terhadap bakteri Multidrug Resistant (MDR) A. baumannii dan P. aeruginosa. Kelopak bunga rosela dimaserasi menggunakan pelarut etanol 96%. Aktivitas antibakteri diuji dengan metode cakram difusi pada konsentrasi 20, 40, 60, 80, dan 100%. Hasil penelitian menunjukkan diameter zona inhibisi ekstrak kelopak bunga rosela pada konsentrasi tersebut terhadap bakteri MDR A. baumannii adalah 10,8, 15, 16,8, 17, dan 21,2 mm, dan terhadap bakteri MDR P. aeruginosa adalah 10,6, 12,2, 16,8, 18, dan 23 mm. Uji statistik menggunakan uji Kruskal-Wallis didapatkan nilai P = 0,000 (P < 0,05) yang menunjukkan perbedaan aktivitas antibakteri yang signifikan antar kelompok konsentrasi ekstrak terhadap bakteri MDR A. baumannii maupun P. aeruginosa. Kesimpulan yang didapat adalah aktivitas antibakteri ekstrak kelopak bunga rosela terhadap bakteri MDR A. baumannii dan P. aeruginosa meningkat seiring dengan peningkatan konsentrasi.
Deteksi Antigen Helicobacter pylori Pada Pasien GERD dan Non-GERD di Rumah Sakit Atma Jaya Kurniawan, Athalia Nofera; Tenggara, Riki; Moehario, Lucky Hartati
Majalah Kedokteran Indonesia Vol 69 No 8 (2019): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.69.8-2019-185

Abstract

Introduction: Helicobacter pylori (H. Pylori) is one of normal human microbiota live in human stomach and often associated with dyspepsia, however, its association with Gastroesophageal Reflux Disease (GERD) is still controversial. The diagnosis of H. pylori is often established through histopathological examination, an invasive urease tests, and urea breath test (UBT. Serological tests are also used to diagnose H. pylori, one of which is Stool Antigen Test (SAT), which is cheaper, non-invasive and easier to operate. The purpose of this study was to detect H. pylori antigen in GERD and non-GERD patients at Atma Jaya Hospital.Method: This study was a descriptive cross-sectional. Participants in this study were adult patients hospitalised in Atma Jaya Hospital from August 2018 - November 2018, interviewed with GERDQ questionnaire and stool samples were collected. Stool Antigen Test was carried out using SAT from Biocare®. Results: Thirty participants were included in this study, consisted of 22 women and 8 men, and predominated by adult age group (35–64 years old). Forty percent participants were GERD group consisted of 75% women. Positive SAT test results in the GERD group was 16.67%, non-GERD was 11.11%, and negative SAT test results in the GERD group was 91.67%, non-GERD was 77.78%. Conclusion: The presence of H. pylori antigen was detected in women in the adult age group. It seemed the presence of H. pylori was not different from GERD group and non-GERD group.
Effectiveness of azithromycin and ciprofloxacin in the treatment of typhoid fever: a systematic review Mulyono, Bethea Manuela; Ita Margaretha Nainggolan; Linawati Hananta; Lucky Hartati Moehario
Indonesian Journal of Pharmacology and Therapy Vol 4 No 3 (2023)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.8945

Abstract

Typhoid fever or commonly referred to as enteric fever is a systemic disease caused by Salmonella typhi. It often occurs in developing countries due to poor sanitation. Second-line antibiotics, including ciprofloxacin, have been widely used as the first choice treatment for typhoid fever. However, S. typhi has increased resistance to second-line antibiotics, so azithromycin has become an alternative treatment for the typhoid fever. This systematic review assessed the effectiveness of azithromycin and ciprofloxacin against typhoid fever in adults. This research used the PRISMA protocol with predefined Pubmed, Proquest, and EBSCO keywords. After removing duplicates and selecting studies according to inclusion and exclusion criterias, 17 studies about the effectiveness of azithromycin and ciprofloxacin in typhoid fever in adults from 1988-2020 were gathered. Studies were evaluated using JBI Critical Appraisal Tools. The results showed that administration of azithromycin and ciprofloxacin for typhoid fever yields average length of fever (3.5 d and 4.2 d) and average length of stay (9.3 d and 10 d), respectively. The susceptibility of S. typhi to azithromycin was 99.9%, whereas a significant decrease in its susceptibility to ciprofloxacin was observed in 2017-2020, from 4% to 2%. Azithromycin showed better effectiveness than ciprofloxacin in treating typhoid fever in adults based on S. typhi’s susceptibility, average length of fever and average length of stay from 17 studies reviewed.
Co-Authors Andi Yasmon Anis Karuniawati Delly Chipta Lestari Elisha Minarto Irene Ita Margaretha Nainggolan Kurniawan, Athalia Nofera Linawati Hananta Mulyono, Bethea Manuela Riki Tenggara Rudyanto Sedono Thomas Robertus