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All Journal AGROTEK: Jurnal Ilmiah Ilmu Pertanian Agroradix : Jurnal Ilmu Pertanian
Rida Iswati
Program Studi Agroteknologi, Fakultas Pertanian, Universitas Negeri Gorontalo
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Uji Patogenisitas Beauveria bassiana Terhadap Larva Spodoptera frugiperda (J.E. Smith) Suslara Santika Manopo; Mohamad Lihawa; Angry Pratama Solihin; Rida Iswati; Silvana Apriliani
AGRORADIX : Jurnal Ilmu Pertanian Vol. 9 No. 2 (2026): Januari-Juli 2026
Publisher : Agroteknologi Fakultas Pertanian Universitas Islam Darul 'Ulum (UNISDA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52166/agroteknologi.v9i2.13159

Abstract

Penelitian ini bertujuan untuk mengevaluasi efektivitas Beauveria bassiana dalam mengendalikan larva Spodoptera frugiperda. Penelitian ini dilakukan pada Maret hingga Juni 2025. Penelitian ini menggunakan empat perlakuan, yaitu suspensi Beauveria bassiana 107, 109, dan 1011  konidia mL-1 serta kontrol, dalam Rancangan Acak Lengkap (RAL) nonfaktorial. Setiap perlakuan diulang lima kali, menghasilkan dua puluh unit percobaan. Variabel yang diamati pada penelitian ini yaitu masa inkubasi jamur Beauveria bassiana, Perkembangan Beauveria bassiana pada jaringan internal Spodoptera frugiperda, mortalitas larva Spodoptera frugiperda dan Aktivitas Makan larva Spodoptera frugiperda. Data penelitian akan dianalisis menggunakan Analisis of Varian (ANOVA), diikuti oleh Uji Duncan Multiple Range Test (DMRT) pada taraf signifikan 5%. Hasil penelitian menunjukan bahwa aplikasi Beauveria bassiana pada seluruh perlakuan berpengaruh nyata dalam mempercepat masa inkubasi terhadap larva Spodoptera frugiperda dibandingkan kontrol, dengan perlakuan 109  konidia mL-1 dan 1011  konidia mL-1 menunjukan waktu yang singkat dibandingkan 107  konidia mL-1. Infeksi Beauveria bassiana pada larva Spodoptera frugiperda bersifat progresif, ditandai dengan tidak terdeteksinya struktur jamur pada 1–4 HSA dan munculnya perubahan jaringan internal pada 5 HSA. Mortalitas larva Spodoptera frugiperda akibat aplikasi Beauveria bassiana mulai terjadi pada 4 HSA, mencapai puncak pada 7 HSA terutama pada perlakuan 109  konidia mL-1, kemudian menurun setelah 8 HSA. Aplikasi Beauveria bassiana pada seluruh perlakuan 107  konidia mL-1, 109  konidia mL-1 dan 1011  konidia mL-1 secara nyata menurunkan aktivitas makan larva Spodoptera frugiperda dibandingkan kontrol.
PEMANFAATAN EKSTRAK AMPAS TAHU SEBAGAI MEDIA Trichoderma brevicompactum DALAM MENEKAN Fusarium sp. DAN MENINGKATKAN PERKECAMBAHAN BENIH CABAI Aldianto S. Amas; Rida Iswati; Nikmah Musa
AGROTEK: Jurnal Ilmiah Ilmu Pertanian Vol 10, No 1 (2026): Maret
Publisher : Percetakan Umi Toaha Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/agrotek.v10i1.1180

Abstract

Chili peppers (Capsicum annuum L.) are a commodity with high economic value and a very high consumption rate in Indonesia. The high demand is not matched by the availability of production, because chili plants are susceptible to Fusarium sp. disease. Farmers control the disease using chemical pesticides, the use of which causes negative impacts such as land degradation, death of natural enemies, and environmental pollution. A more environmentally friendly control alternative is Trichoderma sp., which is able to suppress Fusarium sp. pathogens and has the potential to increase plant growth. In this study, tofu dregs extract was used as a propagation medium for T. brevicompactum. This study aimed to examine the potential of tofu dregs extract as a propagation medium for T. brevicompactum, assess the effectiveness of the formulation in inhibiting Fusarium sp. in vitro, and evaluate its effect on chili seed germination. The research activities were carried out experimentally from April–July 2025 at the Biological Agent Laboratory of BPTPH Gorontalo Province. Observed parameters included media characteristics, growth and sporulation of T. brevicompactum, inhibition against Fusarium sp., and the formulation's ability to increase chili seed germination. Data were analyzed descriptively for the physical properties of the media, using a t test for spore density, and ANOVA followed by a 5% BNJ test for seed growth parameters. The results showed that tofu dregs extract effectively supported the growth and sporulation of T. brevicompactum. The resulting formulation effectively inhibited Fusarium sp. in vitro and increased chili seed viability in in vivo tests, with germination values equivalent to fungicide treatment. Tofu dregs extract has the potential to be a propagation medium for T. brevicompactum and its application as a biopesticide to suppress Fusarium sp. disease and stimulate chili seed germination.
Co-Authors Aldianto S. Amas Angry Pratama Solihin Mohamad Lihawa Nikmah Musa Silvana Apriliani Suslara Santika Manopo