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All Journal Jurnal Pendidikan Biologi Undiksha Jurnal Pembelajaran dan Biologi Nukleus
Khaerunissa Anbar Istiadi
Institut Teknologi Sumatera
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media

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Endophytic Bacteria From Bidara (Ziziphus mauritiana L.) Leaves and Its Activity Against the Pathogens Salmonella typhi and Escherichia coli Mega Dwi Rhama; Dewi Chusniasih; Khaerunissa Anbar Istiadi; Erma Suryanti; Hida Arliani Nur Anisa
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i2.8254

Abstract

Background: One possible source of bioactive substances is endophytic bacteria. These bacteria reside in plant tissues without harming the host plant. By generating unique chemicals in plant tissues and boosting the body's defense mechanisms against host plants, endophytic bacteria can manage infections. The purpose of this study is to investigate and describe the ability of endophytic bacteria from bidara leaves to create antimicrobial substances via their metabolite activities. Methodology: Nutrient Agar (NA) media was used exclusively for the isolation and culture of endophytic bacteria. Endophytic bacterial colonies were morphologically characterized both macroscopically and microscopically. Salmonella typhi and Escherichia coli growth inhibition was evaluated for endophytic bacterial metabolites (supernatants) with disc diffusion method. Chloramphenicol 0.3% used as positive control, and steriled aquadest as negative control. Findings: END01, END02, and END03 are the three endophytic bacterial isolates that were recovered. The three isolates differed in their edges and forms; END01 was Gram negative, whereas END02 and END03 were Gram positive. Isolate END01 and END03 selected for strong inhibition in antagonist tests against S. typhi (16.44 mm and 12.57 mm, respectively) compared to chloramphenicol control (45.86 mm). Their secondary metabolites were harvested at stationary phase, and chemical screening confirmed positive flavonoids in cell-free supernatants. The END01 supernatant showed the highest antimicrobial activity, with inhibition zones of 26.30 mm against S. typhi and 11.34 mm against E. coli, categorized as very strong and strong inhibition, respectively. Contribution: Endophytic bacteria isolated from the bidara leaves have the potential to inhibit the growth of pathogenic Salmonella typhi and Escherichia coli. This study represents the first reported exploration of endophytic bacteria from bidara leaves as flavonoid-producing antimicrobial agents against these clinically significant pathogens
Perbedaan Profil Protein Plasma Darah Mencit Menggunakan SDS-PAGE Tanpa Penambahan dan dengan Penambahan 2-Mercaptoethanol Khaerunissa Anbar Istiadi; Iffa Afiqa Khairani; Silvia Andriani
Jurnal Pendidikan Biologi Undiksha Vol. 11 No. 2 (2024)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v11i2.82139

Abstract

SDS-PAGE is a method used to analyze proteins based on differences in molecular weight. Sample preparation in SDS-PAGE with 2-mercaptoethanol will reduce disulfide bonds in proteins. The sulfide bond reduction process will cause differences in protein conformation and profile. The purpose of this study was to compare the pattern of blood plasma protein profiles of mice analyzed using SDS-PAGE, with the addition of 2-mercaptoethanol and without the addition of 2-mercaptoethanol in a mixture of sample buffer solutions. The results of SDS-PAGE showed that the blood plasma protein profile of mice without the addition and with the addition of 2-mercaptoethanol showed differences in the number of protein bands, the molecular weight of several proteins and the thickness of the protein bands. There were nine protein bands with a molecular weight above 52 kDa in the profile without the addition of 2-mercaptoethanol, while there were only five protein bands with a molecular weight above 52 kDa in the profile with the addition of 2-mercaptoethanol. The difference in protein profile patterns shows that mouse blood plasma contains proteins containing disulfide bonds that can be reduced by 2-mercaptoethanol.
Phytochemical Compound, Antioxidant and Antibacterial Properties of Sargassum sp. Extracts from Pahawang Island, Lampung Province Iffa Afiqa Khairani; Khaerunissa Anbar Istiadi; Nawang Restiawati
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.6558

Abstract

Pahawang Island, located in Lampung Bay, has a variety of potential natural resources, one of which is macroalgae. One of the abundant brown macroalgae is Sargassum sp., with phytochemical components (flavonoids, steroids, tannins, and glycosides) that have potential as antioxidants, antibacterial, antitumor, anticancer, antifouling, and others. Sargassum sp. from Lampung waters, especially Pahawang Island still lacks scientific studies on its bioactive content and properties. This study aims to examine the phytochemical compounds (qualitatively and quantitatively), antioxidant activity (DPPH method) and antibacterial potential of Sargassum sp. extract (MIC/MBC method) against Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The results of phytochemical assay  showed that Sargassum sp. extract contained phenolic compounds, flavonoids, tannins, and saponins. Quantification results on total phenolic content were 0.56 mgGAE/g extract, total tannins were 4.27 mgTAE/g extract, total flavonoids were 14.19 mgQE/g extract, and total saponins were 5.54 %. Sargassum sp. extract classified as a very weak antioxidant with IC50 value of 964.10 ppm, and moderate antibacterial activity at a concentration of MIC/MBC 250/250 ppm against B. subtilis, E. coli, P. aeruginosa, and S. aureus
Unveiling the Antimicrobial of Lactic Acid Bacteria from Gatot Cassava Against Escherichia coli and Salmonella typhi Dewi Chusniasih; Nadia Alfauzia; Erma Suryanti; Khaerunissa Anbar Istiadi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.7161

Abstract

Background: Lactic Acid Bacteria (LAB) as probiotics provide many benefits to the body, including suppressing the growth of pathogens in the digestive tract. Antibacterial compounds from LAB can inhibit the presence of pathogenic bacteria. This study aimed to determine the ability of antibacterial compounds from Gatot LAB to inhibit the growth of Escherichia coli and Salmonella typhi bacteria. Methodology: LAB was isolated from cassava gatot, and The LAB isolates obtained were then tested for several LAB characteristics and antibacterial activity against E. coli and S. typhi. LAB characterization based on colony morphology, Gram staining, biochemical testing, coaggregation, and autoaggregation. Findings: There were 7 LAB isolates obtained, namely isolates with codes GNK1, GNK3, GNR2, GNR4, GUR1, GUR3, and GUR5. Isolate GUR3 can inhibit E. coli with the highest inhibition zone of 21.31 ± 3.98 mm. In the antibacterial test against S. typhi, 4 isolates had an inhibitory activity with the highest inhibition diameter by isolate GNK3 of 28.23±3.91 mm. LAB isolates from gatot have the ability to autoaggregate and coaggregate against E. coli and S. typhi. There are 5 isolates that show inhibitory activity against E. coli, namely GNK1, GNK3, GNR2, GNR4, GUR1, and GUR3. Inhibitory activity against S. typhi is shown by 4 LAB isolates, namely GNK1, GNK3, GNR2, and GNR4. Contribution: This research shows that LAB isolates from gatot have potential as probiotic agents with significant antibacterial activity against E. coli and S. typhi, so it can be developed as a natural alternative to control intestinal pathogens.
Co-Authors Dewi Chusniasih Erma Suryanti Erma Suryanti Hida Arliani Nur Anisa Iffa Afiqa Khairani Mega Dwi Rhama Nadia Alfauzia Nawang Restiawati Silvia Andriani