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All Journal Jurnal Pendidikan Biologi Undiksha Jurnal Ilmiah Kesehatan Jurnal Biosense Quagga: Jurnal Pendidikan dan Biologi
Silvia Andriani
Universitas Muhammadiyah Pringsewu
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Antioxidant Activity and Immunomodulatory Effect of Stachytarpheta sp. Leaf Extract on Phagocytic Activity of Mice Peritoneal Macrophages in Alloxan Induction Iffa Afiqa Khairani; Wahyuni Dian Lestari; Elisa Nurma Riana; Jeane Siswitasari Mulyana; Hida Arliani Nur Anisa; Rizka Fatriani; Silvia Andriani
Quagga: Jurnal Pendidikan dan Biologi Vol 17 No 2 (2025): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v17i2.404

Abstract

Hyperglycemia is a condition characterized by elevated blood glucose levels, which is a hallmark of diabetes mellitus. Prolonged hyperglycemia not only disrupts glucose homeostasis but also impairs the immune response. The condition of hyperglycemia in the body can increase free radicals and oxidative stress, thus can impair various macrophage functions, including their ability to engulf and eliminate pathogens. The bioactive constituents in Stachytarpheta sp. may contribute to reducing oxidative damage and enhancing immune system function. This study aims to evaluate both the antioxidant capacity and the immunomodulatory effects of Stachytarpheta sp. leaf extract on the phagocytic activity of peritoneal macrophages in alloxan-induced diabetic mice. Alloxan is widely used in research to induce hyperglycemia (diabetic conditions) in experimental animals. The antioxidant activity of the extract was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay, while phagocytic activity of peritoneal macrophages was performed in vivo using male mice. Stachytarpheta sp. leaf extract from Beteng Sari Village, East Lampung has very strong antioxidant activity against DPPH with IC50 value 14,85 ± 0,30 μg/mL. This extract at doses of 200, 300, and 400 mg/kgBW can be an immunomodulator that maintains the phagocytic activity of macrophage cells in alloxan-induced mice.
ANALISIS BIOMARKER C-REACTIVE PROTEIN (CRP) PADA PENYAKIT DIABETES MELITUS Egita Windrianatama Puspa; Silvia Andriani; Gustiadi Saputra
Jurnal Ilmiah Kesehatan Vol. 15 No. 1 (2026): Jurnal Ilmiah Kesehatan
Publisher : Universitas Muhammadiyah Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52657/jik.v15i1.3396

Abstract

Hiperglikemia kronis pada penderita gangguan metabolisme glukosa memicu respon peradangan sistemik yang meningkatkan risiko komplikasi pembuluh darah. Penelitian ini bertujuan menganalisis korelasi antara kadar Glukosa Darah Puasa dengan biomarker peradangan C-Reactive Protein. Menggunakan desain analitik observasional dengan pendekatan potong lintang, penelitian ini melibatkan sampel sebanyak 35 subjek. Kadar Glukosa Darah Puasa diukur dengan metode enzimatik, sedangkan status reaktivitas dan titer C-Reactive Protein ditentukan melalui uji aglutinasi lateks semi-kuantitatif. Hasil penelitian menunjukkan mayoritas subjek (37,1%) berada pada kategori hiperglikemia berat dengan prevalensi C-Reactive Protein positif mencapai 100%. Rata-rata kadar Glukosa Darah Puasa adalah 147,2 mg/dL dan kadar C-Reactive Protein sebesar 18,4 mg/L. Uji korelasi Spearman’s Rho menunjukkan hubungan signifikan yang sangat kuat p-value = 0,000 (p < 0,05). Hal ini membuktikan bahwa peningkatan kadar Glukosa darah secara masif mengaktivasi jalur peradangan sistemik melalui mediator pro-inflamasi, sehingga memperburuk status kesehatan subjek. Dapat disimpulkan bahwa semakin buruk kontrol glikemik, semakin tinggi derajat peradangan sistemik yang terjadi.
Perbedaan Profil Protein Plasma Darah Mencit Menggunakan SDS-PAGE Tanpa Penambahan dan dengan Penambahan 2-Mercaptoethanol Khaerunissa Anbar Istiadi; Iffa Afiqa Khairani; Silvia Andriani
Jurnal Pendidikan Biologi Undiksha Vol. 11 No. 2 (2024)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v11i2.82139

Abstract

SDS-PAGE is a method used to analyze proteins based on differences in molecular weight. Sample preparation in SDS-PAGE with 2-mercaptoethanol will reduce disulfide bonds in proteins. The sulfide bond reduction process will cause differences in protein conformation and profile. The purpose of this study was to compare the pattern of blood plasma protein profiles of mice analyzed using SDS-PAGE, with the addition of 2-mercaptoethanol and without the addition of 2-mercaptoethanol in a mixture of sample buffer solutions. The results of SDS-PAGE showed that the blood plasma protein profile of mice without the addition and with the addition of 2-mercaptoethanol showed differences in the number of protein bands, the molecular weight of several proteins and the thickness of the protein bands. There were nine protein bands with a molecular weight above 52 kDa in the profile without the addition of 2-mercaptoethanol, while there were only five protein bands with a molecular weight above 52 kDa in the profile with the addition of 2-mercaptoethanol. The difference in protein profile patterns shows that mouse blood plasma contains proteins containing disulfide bonds that can be reduced by 2-mercaptoethanol.
EFEK EKSTRAK KULIT BUAH KABAU (Archidendron bubalinum (Jack) I.C Nielsen) TERHADAP AKTIVITAS MAKROFAG MENCIT JANTAN SETELAH DIPAPARKAN CdCl2 Elisa Nurma Riana; Iffa Afiqa Khairani; Hida Arliani Nur Anisa; Rizka Fatriani; Silvia Andriani
JURNAL BIOSENSE Vol 9 No 2 (2026): Edisi April 2026
Publisher : Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i2.7817

Abstract

Logam kadmium (Cd) yang berasal dari kegiatan industri seperti produksi baterai, pelapisan logam, penggunaan pupuk fosfat, pembakaran bahan bakar fosil, dan pembuatan plastik dapat meningkatkan produksi reactive oxygen species (ROS). Produksi ROS berlebih dalam tubuh akan menyebabkan penyakit dan mempengaruhi sistem imun tubuh akibat stres oksidatif. Stres oksidatif merupakan suatu kondisi ketidakseimbangan antara radikal bebas dan antioksidan. Untuk melindungi tubuh dari stres oksidatif diperlukan suatu senyawa antioksidan, seperti yang terkandung dalam kulit buah kabau (Archidendron bubalinum). Penelitian ini bertujuan mengetahui mengetahui adanya peningkatan aktivitas fagositosis makrofag pada mencit Jantan yang dipaparkan CdCl2. Penelitian ini bersifat eksperimental menggunakan rancangan acak lengkap (RAL). Mencit yang digunakan 24 ekor dengan 6 kelompok yang terdiri dari 3 kelompok kontrol dan 3 kelompok perlakuan. Hasil pengujian menunjukkan bahwa pemberian ekstrak kulit buah kabau dosis 95 mg/kgBB, 190 mg/kgBB dan 380 mg/kgBB pada uji aktivitas fagositosis makrofag menunjukkan hasil yang signifikan sebesar p = 0,005. Hal ini berarti bahwa ekstrak kulit buah kabau dapat meningkatkan sistem imun mencit dengan perlakuan P2 memiliki aktivitas fagositosis tertinggi yaitu 55,91%.
Co-Authors EGITA WINDRIANATAMA PUSPA Gustiadi Saputra Hida Arliani Nur Anisa Hida Arliani Nur Anisa Iffa Afiqa Khairani Jeane Siswitasari Mulyana Khaerunissa Anbar Istiadi Riana, Elisa Nurma Rizka Fatriani Wahyuni Dian Lestari