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MALFORMASI BAGIAN – BAGIAN TUBUH FETUS MENCIT (Mus musculus L.) SETELAH PEMBERIAN EKSTRAK BUAH PARE (Momordica charantia L.) Silvia Andriani; Nuning Nurcahyani; Hendri Busman; Evi Kurniawaty; Iffa Afiqa Khairani
Jurnal Ilmiah Kesehatan Vol 12, No 1 (2023): Jurnal Ilmiah Kesehatan
Publisher : Universitas Muhammadiyah Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52657/jik.v12i1.2136

Abstract

Pare memiliki khasiat sebagai tanaman obat tradisional. Pada buah pare terdapat beberapa senyawa yang bersifat toksik yaitu senyawa momordikosida K dan L, yang diduga mempunyai sifat sitotoksik. Penelitian ini bertujuan untuk mengetahui panjang kranium, dan panjang sternum fetus mencit (Mus musculus L.) setelah pemberian ekstrak buah pare (Momordica charantia L.) terhadap mencit yang sedang hamil. Ekstrak pare diperoleh dengan cara maserasi menggunakan etanol 95%. Pemberian ekstrak buah pare (M. charantia L.) sebagai uji teratogenik pada kelainan fetus mencit. Rancangan penelitian adalah rancangan acak lengkap dengan analisis varian, yaitu menggunakan 20 ekor mencit jantan dan 20 ekor mencit betina yang dibagi dalam 4 kelompok. Setiap kelompok terdiri dari 5 ekor mencit betina yang telah hamil di beri ekstrak buah pare secara oral dimulai hari ke - 6 sampai hari 17 masa kehamilan sehari sekali pada pagi hari dengan dosis Perlakuan, aquabidest sebagai kontrol [A], [B] 22,5 mg/30 grBB , [C] 30 mg/30 grBB, dan [D] 37,5 mg/30 grBB . Selanjutnya induk mencit dibedah diambil fetusnya untuk dilakukan pengukuran terhadap panjang kranium dan panjnag sternum. Dari hasil analisis data menunjukkan bahwa secara umum pemberian perlakuan ekstrak buah pare menyebabkan penurunan rata-rata panjang kranium,dan sternum. Hasil analisis lanjut dengan BNT pada taraf 5 % menunjukkan bahwa pemberian dosis [B], [C], [D] memberikan pengaruh yang nyata terhadap penurunan panjang kranium, dan sternum. Pemberian ekstrak buah pare pada mencit hamil dosis yang paling efektif untuk menurunkan panjang kranium dan panjang sternum yaitu dosis 37,5 mg/30 grBB.
PEMERIKSAAN GOLONGAN DARAH DAN RHESUS PADA MASYARAKAT DESA SENDANG MULYO KECAMATAN SENDANG AGUNG KABUPATEN LAMPUNG TENGAH Andriani, Silvia; Puspa, Egita Windrianatama; Sahroni, Mizan; Arif, Muhammad; Sandro, Mario; Putra, Yogi Catur; Kurniawaty, Evi; Khairani, Iffa Afiqa
Bagimu Negeri Vol 7, No 2 (2023)
Publisher : Universitas Muhammadiyah Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52657/bagimunegeri.v7i2.2181

Abstract

Darah merupakan salah satu komponen paling penting yang ada dalam tubuh, mengingat fungsinya sebagai alat transportasi. Pemeriksaan golongan darah adalah salah satu aspek dalam upaya pencegahan penyakit. Identifikasi golongan darah dengan melihat penggumpalan darah yang telah diberi reagen banyak dilakukan secara manual berdasarkan pengamatan visual secara langsung. Pemeriksaan golongan darah dapat menjadi informasi penting dalam konteks perawatan kesehatan, terutama dalam situasi darurat atau ketika transfusi darah diperlukan. Indonesia masih mengalami kekurangan jumlah penyediaan darah secara nasional sebanyak 500 ribu kantong. Kekurangan ketersediaan darah tersebut meliputi golongan darah langka seperti golongan darah AB dan rhesus negatif. Pengabdian masyarakat adalah suatu kegiatan yang bertujuan untuk membantu masyarakat dalam meningkatkan pengetahuan dan derajat kesehatan. Kegiatan pengabdian masyarakat menjadi salah satu bagian dari Catur Dharma Perguruan Tinggi. Tujuan kegiatan pengabdian masyarakat ini adalah untuk mengetahui hasil pemeriksaan golongan darah dan rhesus pada masyarakat di desa Sendang Mulyo Kecamatan Sendang Agung. Desain kegiatan ini menggunakan desain deskriptif observational yaitu menggambarkan hasil pemeriksaan golongan darah berdasarkan hasil observasi dilapangan. Pemeriksaan Golongan darah sistem ABO yang dilakukan di Desa Sendang Mulyo diperoleh hasil golongan darah A sebanyak 9 orang, golongan darah B sebanyak 18  orang, golongan darah O sebanyak 24 orang, sedangkan untuk golongan darah AB sebanyak 4 orang
Deteksi Konsentrasi Fe, Cu, Zn dan Pb Air Sungai dan Ikan yang Tercemar Air Lindi di Bandar Lampung Dengan Menggunakan SSA: Detections of Fe, Cu, Zn and Pb Concentration in River Water and Fish Polluted by Leachate in Bandar Lampung Using AAS Andriyana, Ahmad Dodi; Mulyana, Jeane Siswitasari; Khairani, Iffa Afiqa; Riana, Elisa Nurma
Jurnal Sumberdaya Hayati Vol. 10 No. 2 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.2.97-101

Abstract

Leachate is a pollutant produced by landfills which can pollute the aquatic environment. This will have an impact on fish that live in waters contaminated with leachate and if consumed by the public it will harm health. The toxic effects of leachate are caused by the content of toxic chemicals, one of which is heavy metals. The aim of this research was to determine the concentrations of Fe, Cu, Zn and Pb in Bakung landfill leachate, Sukamaju river water and fish meat. Bakung landfill leachate samples were taken from two outlet points. The fish sampling location was carried out in the Sukamaju River. The physical factors of water measured are temperature, pH, salinity, turbidity, DO, COD and BOD. Fe, Cu, Zn and Pb concentrations were measured using the Atomic Absorption Spectrophotometry (AAS) method. The results show that the physicochemical parameters in leachate and river water exceed the threshold. The contents of Zn, Cu, Fe and Pb in the leachate at both outlet points exceeded the threshold value with values of 2.48 mg/L and 2.80 mg/L; 16.55 mg/L and 17 mg/L; 0.078 mg/L and 0.113 mg/L; 3.26 mg/L and 3.01 mg/L. The concentration of heavy metals Pb and Fe in river water is 0.083-0.162 mg/L and 2.68-2.97 mg/L which are above the quality standard value, while the heavy metal Fe content in fish meat exceeds the specified threshold value, namely 0.557 mg/100 g and 0.564 mg/100 g.
Effect Of Compost Tea and JADAM Microorganism Solution On Growth Of Chili Pepper In PT. Cinquer Agro Nusantara Khairani, Iffa Afiqa; Novriadi, Novriadi; Wandasari, Septi Putri; Nanda, M. Zulhiyadi; Anshori, Andi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i1.3807

Abstract

Chili pepper (Capsicum frustescens) is herbaceous plant from the family Solanaceae which is massively cultivated in Indonesia. It also can be used as raw material for food product and pharmacy industries. Organic fertilizer usage has been both a solution to increase productivity and a replacement to chemical fertilizer usage. The aim of this study is to examine the effect of compost tea organic fertilizer and JADAM Microorganism Solution (JMS) on the growth of chili pepper in PT. Cinquer Agro Nusantara, Bangka Belitung. This research used a completely randomized design with 15 unit treatments which were divided into control group, compost tea group, and JMS group. The result showed that the plant group with JMS performed the highest height expansion, increasing the number of leaves significantly compared to control and compost tea group. This study concludes that application of compost tea and JADAM Microorganism Solution (JMS) as organic fertilizer on C. frustescens can increase the plant’s growth.Keywords : chili pepper, compost tea, JADAM microorganism solution
Sosialisasi Potensi Etnobotani dan Etnozoologi Desa Pahawang Maretta, Gres; Nurhayu, Winati; Novriadi, Novriadi; Yanti Ariyanti, Yanti Ariyanti; Wahyuni, Kurnia; Riana, Elisa Nurma; Khairani, Iffa Afiqa; Darmawan, Andy; Leksikowati, Sovia Santi; Kesuma, Andri Jaya
Abdimas Galuh Vol 7, No 1 (2025): Maret 2025
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/ag.v7i1.17818

Abstract

Etnobiologi adalah evaluasi ilmiah terhadap pengetahuan penduduk tentang biologi, meliputi pengetahuan tentang tumbuhan (etnobotani), hewan (etnozoologi) dan lingkungannya (etnoekologi). Kajian etnobiologi dapat menghubungkan biologi dan etnis yang ada pada suatu tradisi adat. Melalui kegiatan Pengabdian Kepada Masyarakat, mendorong masyarakat untuk peduli terhadap pelestarian sumber daya alam yang ada di Desa Pahawang. Hasil akhir dari pelaksanaan kegiatan adalah pengetahuan masyarakat tentang pemanfaatan etnobotani dan etnozoologi yang ada di desa Pahawang. Kami melakukan penilaian terhadap pengetahuan dan ketertarikan masyarakat mengenai pemanfaatan etnobotani dan etnozoologi yang ada dan diterapkan di desa Pahawang. Pengetahuan masyarakat mengenai potensi dan pemanfaatan etnobotani dan etnozoologi di desa Pahawang meningkat setelah pelatihan jika dibandingkan dengan sebelum pelatihan. Hal ini dapat dilihat dari ketercapaian tujuan dari kegiatan dari hasil pengolahan data kuesioner. Rerata ketercapaian tujuan dapat dikatakan baik (75,6%) berdasarkan dari hasil pre-test dan post-test, dimana terdapat peningkatan pengetahuan dari ibu-ibu peserta kegiatan sebesar dari awal hingga akhir kegiatan. Kemampuan melakukan budidaya tanaman pangan, dan pengetahuan tentang pengobatan alami merupakan potensi besar untuk dikembangkan demi meningkatkan ketahanan pangan dan kesehatan yang lebih baik bagi masyarakat Desa Pahawang ke depan.
Pelatihan Pemanfaatan Limbah Minyak Jelantah menjadi Produk Lilin Aromaterapi di Desa Pahawang, Pesawaran Khairani, Iffa Afiqa; Anbar Istiadi, Khaerunissa; Chusniasih, Dewi; Suryanti, Erma; Arliani Nur Anisa, Hida; Natalia Pardede, Desta
Jurnal Abdimas Berdaya : Jurnal Pembelajaran, Pemberdayaan dan Pengabdian Masyarakat Vol 8, No 1 (2025): Jurnal Abdimas Berdaya
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/jab.v8i1.1040

Abstract

IMMUNOSTIMULATORY EFFECT OF VITAMIN C ON MICE INDUCED BY STAPHYLOCOCCUS AUREUS Khairani, Iffa Afiqa; Riana, Elisa Nurma; Maretta, Gres; Azizah, Najla Nur; Putri, Isnaini Anggraini; Maruli, Berkat Afni; Mulyadi, Salsa Pratiwi; Antonius, Antonius
MAXIMUS: Journal of Biological and Life Sciences Vol. 1 No. 2 (2023)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35472/maximus.v1i2.1636

Abstract

Several compounds that can boost immune activity (immunostimulatory) include vitamin E, curcumin, flavonoids, and vitamin C. In this study, ascorbic acid or vitamin C, was used as one method to enhance the immune system. This study aims to determine the immunostimulatory effect of vitamin C on the phagocytosis activity of macrophages in mice induced by Staphylococcus aureus. The mice were divided into two groups: the control group, which received 0.3 ml of distilled water orally, and the treatment group, which received 0.036 mg/g BW of vitamin C orally for 14 days. On the 15th day, each mouse was intraperitoneally induced with 0.5 mL of Staphylococcus aureus bacterial suspension and left for two hours. The phagocytic activity value represents the percentage of active macrophage cells among all macrophages observed. The result showed that the value of % phagocytic activity in the control group was 21.43% ± 7.92, whereas the % phagocytic activity in the treatment group was 48.31% ± 22.27. The group of mice given vitamin C 0.036 mg/g BW orally showed increased phagocytic activity compared to the control group mice. This shows that vitamin C has potential as an immunostimulator.
Quantitative Analysis of Phytochemical Compounds and Antihyperglycemic Potential of Robusta Coffee from West Lampung Khairani, Iffa Afiqa; Mulyana, Jeane Siswitasari; Olivia, Rahel Noviantika; Riana, Elisa Nurma; Anisa, Hida Arliani Nur
Jurnal Sumberdaya Hayati Vol. 10 No. 1 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.1.1-6

Abstract

West Lampung Regency in Lampung Province produces high quality robusta coffee with a distinct, strong bitter flavour. Bitter flavour indicates the amount of bioactive substances produced by plants called phytochemicals. The aim of this study is to analyse the phytochemical compounds and antihyperglycemic potential of robusta coffee beans from West Lampung, Indonesia. Quantitative phytochemical analysis was carried out using the thin layer chromatography method and spectrophotometry. Animal experimental design using robusta coffee on hyperglycemia conditions in mice which was induced by alloxan 170 mg/kgBW subcutaneously and given by 1 ml of brewed robusta coffee for 10 days. The robusta coffee from West Lampung contained total caffeine 4,014.87 µg/g, total flavonoid content (TFC) 93.6 mg quercetin equivalent per gram of sample, with total alkaloid content (TAC) 0.848 mg of quinine equivalent per gram of sample, total tannin content (TTC) 182.3 mg of tannic acid equivalent per gram of sample, and total saponin content (TSC) 24.2 mg of quillaja bark equivalent per gram of sample. The administration of 1 ml/mice/day of robusta coffee brewed for 10 days did not show a decrease in blood sugar level in hyperglycemic mice, due to the short duration of the study, so the role of coffee in decreasing hyperglycemia conditions has not been optimally observed.
Antioxidant Activity and Immunomodulatory Effect of Stachytarpheta sp. Leaf Extract on Phagocytic Activity of Mice Peritoneal Macrophages in Alloxan Induction Iffa Afiqa Khairani; Wahyuni Dian Lestari; Elisa Nurma Riana; Jeane Siswitasari Mulyana; Hida Arliani Nur Anisa; Rizka Fatriani; Silvia Andriani
Quagga: Jurnal Pendidikan dan Biologi Vol 17 No 2 (2025): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v17i2.404

Abstract

Hyperglycemia is a condition characterized by elevated blood glucose levels, which is a hallmark of diabetes mellitus. Prolonged hyperglycemia not only disrupts glucose homeostasis but also impairs the immune response. The condition of hyperglycemia in the body can increase free radicals and oxidative stress, thus can impair various macrophage functions, including their ability to engulf and eliminate pathogens. The bioactive constituents in Stachytarpheta sp. may contribute to reducing oxidative damage and enhancing immune system function. This study aims to evaluate both the antioxidant capacity and the immunomodulatory effects of Stachytarpheta sp. leaf extract on the phagocytic activity of peritoneal macrophages in alloxan-induced diabetic mice. Alloxan is widely used in research to induce hyperglycemia (diabetic conditions) in experimental animals. The antioxidant activity of the extract was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay, while phagocytic activity of peritoneal macrophages was performed in vivo using male mice. Stachytarpheta sp. leaf extract from Beteng Sari Village, East Lampung has very strong antioxidant activity against DPPH with IC50 value 14,85 ± 0,30 μg/mL. This extract at doses of 200, 300, and 400 mg/kgBW can be an immunomodulator that maintains the phagocytic activity of macrophage cells in alloxan-induced mice.
Dampak Kualitas Tidur terhadap Sindrom Pramenstruasi pada Perempuan di Tiga Kecamatan Kabupaten Agam, Sumatera Barat Rara Mulia Sari; Winati Nurhayu; Iffa Afiqa Khairani; Andy Darmawan
Jurnal Penelitian Pendidikan IPA Vol 11 No 12 (2025): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i12.13021

Abstract

Premenstrual Syndrome (PMS) significantly impacts reproductive-age females, yet the interplay of lifestyle factors in rural contexts remains under-researched. This study aimed to analyze the relationship between sleep quality and PMS severity among females in Agam Regency, West Sumatra. A quantitative survey was conducted with 124 participants selected via purposive sampling. Data were collected using the Pittsburgh Sleep Quality Index (PSQI) and the Premenstrual Symptoms Screening Tool (PSST), then analyzed using Linear Models (LM) in R. Results showed that 84.67% of respondents reported poor sleep quality. LM analysis revealed that poor sleep quality significantly predicted severity across all PMS categories: PMDD (p = 0.0054), psychological/somatic symptoms (p = 0.0013), and daily dysfunction (p = 0.0007). Additionally, higher BMI was linked to increased PMDD symptoms (p = 0.0290), while stress significantly influenced daily functional impairment (p = 0.0234). In conclusion, poor sleep quality is a primary predictor of severe PMS, exacerbated by high BMI and stress. These findings suggest that sleep hygiene and stress management are critical non-pharmacological interventions for improving menstrual health in rural populations.