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All Journal Journal of Tropical Mycorrhiza
Wiwin Rahmawati Nurdin
Forestry Department, Faculty of Forestry and Environmental Sciences, Universitas Halu Oleo, Kendari, Indonesia.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Diversity of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Xanthostemon petiolatus (Valeton) Peter G. Wilson at the Halu Oleo University Botanical Garden, Kendari Husna Husna; La Ode Bin Lacang; Faisal Danu Tuheteru; Asrianti Arif; Wiwin Rahmawati Nurdin
Journal of Tropical Mycorrhiza Vol. 5 No. 1 (2026): April
Publisher : Asosiasi Mikoriza Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58222/jtm.v5i1.117

Abstract

Arbuscular mycorrhizal fungi (AMF) are one type of mycorrhizal association with plant roots. AMF have a fungal structure in the form of arbuscules, vesicles, and hyphae, which are found in roots and spores in the soil. The research aimed to determine the diversity of arbuscular mycorrhizal fungi on the rhizosphere of Xanthostemon petiolatus in the Botanical Gardens of Universitas Halu Oleo (UHO), Kendari. Studies that have been conducted show that Nona wood has a symbiotic relationship with AMF. The soil and plant root sampling method was carried out at the Botanical Gardens UHO Kendari, randomly (non-proportionally) based on the distribution of plants. Isolated plant roots and identification of AMF were carried out at the Plastic House of the Indonesian Mycorrhiza Association (AMI) Kendari Branch, Southeast Sulawesi Province, which took place during May-June 2024. The results of the research showed that X. petiolatus roots were colonized by AMF, as evidenced by the discovery of internal hyphae and external hyphae. A total of 6 species of AMF from the genus Glomus (5 species) and the genus Acaulospora (1 species) were found. The dominant type is Glomus. Diversity is included in the low category (H ' = 0.235). This type of AMF has the potential to be developed as a biofertilizer.
Single-Spore Culture of Racocetra crispa Using a Legume Tree Species Faisal Danu Tuheteru; Deli; Husna Husna; Asrianti Arif; Wiwin Rahmawati Nurdin
Journal of Tropical Mycorrhiza Vol. 5 No. 1 (2026): April
Publisher : Asosiasi Mikoriza Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58222/jtm.v5i1.118

Abstract

The propagation of Racocetra crispa using tree-like legume host plants has not yet been widely undertaken. This study aims to investigate the sporulation of R. crispa in single-spore cultures using legume tree host plants, namely sengon (Paraserianthes falcataria), albizia (Albizia saponaria), johar (Cassia siamea), cajanus (Cajanus cajan), and lamtoro (Leucaena leucocephala). The study was conducted at the Greenhouse of the Indonesian Mycorrhiza Association (AMI) Southeast Sulawesi Branch and the Forestry Department Laboratory of Halu Oleo University from November 2025 to February 2026. The study employed a completely randomised design (CRD) with 5 treatments and 3 replicates. The variables observed included culture success, AMF colonisation of the roots, and the number of spores formed at 3 months, 5 months, and after drying 10 g of medium. The results of the study showed that the A. saponaria host plant treatment yielded the best results, with a culture success rate of 86.67%, the highest AMF colonisation of 35.83%, and the highest number of spores at 5 months and after drying 10g of medium. Conversely, L. leucocephala showed the lowest results, with a culture success rate and spore count of 0%. The best host plant for the propagation of Racocetra crispa is A.saponaria.
Co-Authors Asrianti Arif Deli Faisal Danu Tuheteru Husna Husna Husna Husna La Ode Bin Lacang