<![CDATA[ABSTRACTThis study aimed to determine the effectiveness of liquid organic fertilizer (LOF) derived from anchovy (Stolephorus sp.) boiling wastewater fermented with EM4 on the growth of spinach (Amaranthus sp.). The LOF was formulated using five EM4 concentrations (5%, 10%, 15%, 20%, and 25%) along with a control without LOF. The observed parameters included fresh weight, dry weight, and water content of spinach plants during approximately 30 days of cultivation. The results showed that the 5% EM4 treatment (P1) produced the most favorable effect, yielding a fresh weight of 5.4 g and a dry weight of 0.57 g. ANOVA indicated that the treatments had a significant effect on spinach growth. Despite its relatively low nutrient content, the LOF from anchovy boiling wastewater demonstrated effectiveness, presumably due to the presence of bioactive compounds and the activity of fermentative microorganisms.Keywords: Spinach, EM4, Fish Wastewater, Liquid Organic Fertilizer, Plant Growth ABSTRAKPenelitian ini bertujuan untuk mengetahui efektivitas pupuk organik cair (POC) dari limbah air rebusan ikan teri yang difermentasi menggunakan EM4 terhadap pertumbuhan tanaman bayam (Amaranthus sp.). POC diformulasi dengan lima konsentrasi EM4 (5%, 10%, 15%, 20%, dan 25%) serta satu kontrol tanpa POC. Parameter yang diamati adalah berat basah dan berat kering tanaman serta kadar air bayam selama ±30 hari masa tanam. Hasil menunjukkan bahwa perlakuan EM4 5% (P1) memberikan pengaruh terbaik, dengan berat basah 5,4 g dan berat kering 0,57 g. Hasil uji ANOVA menunjukkan bahwa perlakuan berpengaruh nyata terhadap pertumbuhan bayam. POC dari limbah ikan teri menunjukkan efektivitas meskipun unsur haranya rendah, diduga karena keberadaan senyawa bioaktif dan aktivitas mikroorganisme fermentatif.Kata kunci: Bayam, EM4, Limbah Ikan, POC, Pertumbuhan Tanaman]]>
