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All Journal Klinikal Sains (Jurnal Analis Kesehatan)
A.S. Sinulingga
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology

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UJI AKTIVITAS ANTIOKSIDAN EKSTRAK AIR TANAMAN SEMANGGI (Marsilea crenata C.Presl) METODE FRAP (Ferric Reducing Antioxidant Power) raisya adila adila; I.A.K. Pramushinta; A.S. Sinulingga
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4952

Abstract

The clover plant (Marsilea crenata C.Presl) is a plant that is widely used for food and has the potential to be developed as a herbal medicine. Clover plant extract using methanol, chloroform, and ethyl acetate was found to have weak antioxidant activity. The aim of this research was to test the antioxidant activity in air solvent extracts of clover plants using the FRAP (Ferric Reducing Antioxidant Power) method and analyze the effect of variations in extract concentration on the antioxidant activity test. The method in this research is clover plant extraction by maceration using an air solvent. Test antioxidant activity using the FRAP method using a UV-Vis spectrophotometer with a maximum wavelength of 596 nm. The extract yield of 42% showed that it positively contained flavonoids, saponins, polyphenols and a water content of 9.94% ± 0.1276. The results showed that clover plants had weak antioxidant activity (IC50 351.04 ppm) compared to vitamin C as a positive control which showed very strong antioxidant activity (IC50 22.60 ppm). From the results of this study, it can be said that increasing the extract concentration can increase the percentage of inhibition of free radical formation, but the increase is not linear, as indicated by the R2 value not approaching 0.99.
ANALISIS PERBANDINGAN KADAR FLAVONOID TOTAL TEH HIJAU DAN TEH HITAM DENGAN MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS Antonia Vani Kurniawati; .A.K Pramushinta; A.S. Sinulingga; Novamei Indriani
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4986

Abstract

Tea leaves (Camellia sinensis) are a functional plant that is useful for preventing cancer, maintaining immunity and preventing premature aging. Flavonoids are one of the largest groups of polyphenols which are active compounds from tea leaves (Camellia sinensis). This study aims to determine the total flavonoid content of green tea leaf extract and black tea leaf extract using 2 extraction methods, namely maceration with 96% ethanol solvent and infusion using water solvent. The method in this research uses qualitative tests (Wilstarter cyanidin and NaOH 10%) and quantitative tests (UV-Vis Spectrophometry) and conclusions will be drawn using the SPSS statistical test T (independent)-test. The results of the qualitative test using the starter cyanidin and 10% NaOH were positive for containing flavonoids due to a reddish color change. In the quantitative test, the assay was carried out using UV-Vis spectrophotometry with a wavelength of 434 nm. In this study, the total flavonoid content obtained in the green tea maceration method was 15.728% w/w ± 0.209, black tea maceration 13.357% w/w ± 0.737, green tea infusion 4.153% w/w ± 0.124 and black tea infusion 3.320% w /b ± 0.352. There is a significant difference between green tea and black tea using both the infusion and maceration methods as shown by a sig value ≤ 0.05.
Co-Authors .A.K Pramushinta Antonia Vani Kurniawati I.A.K. Pramushinta Novamei Indriani raisya adila adila