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All Journal International Journal of Science and Environment
Fatmawati
Aquaculture Study Program, Faculty of Fisheries and Marine Sciences, Lambung Mangkurat University, Banjarbaru, South Kalimantan, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Mathematics Physics

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Active Compounds In The Aqueous Extract Of Ludwigia Octovalvis Leaves As Antibacterial Agents Against Pathogens Siti Aminah; Siti Aisiah; Fatmawati
International Journal of Science and Environment (IJSE) Vol. 6 No. 2 (2026): May 2026
Publisher : CV. Inara in Colaboration with www.stie-sampit.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51601/ijse.v6i2.687

Abstract

Ludwigia octovalvis is a tropical aquatic weed with potential as a medicinal plant due to its diverse secondary metabolite content. This study aimed to quantify the secondary metabolite profile of aqueous extracts of Ludwigia octovalvis leaves and evaluate their potential as natural antibacterial agents. Leaves were collected from wetlands in Tungkaran Village, Martapura, Banjar Regency, South Kalimantan, and extracted using distilled water at a ratio of 1:4. Quantitative phytochemical analysis was performed in triplicate for saponins, alkaloids, flavonoids, terpenoids, tannins, steroids, anthocyanins, and phenolics. Ludwigia octovalvis leaf extract contains secondary metabolite compounds, namely saponins (15.587%), alkaloids (6.507), flavonoids (91.667 mg/g), terpenoids (228.467 mg/g), tannins (0.850 mg/g), steroids (39.177 mg/g), anthocyanins (negative), and phenolics (65.467 mg/g). The highest levels of secondary metabolites are found in terpenoid compounds, namely 228.467 mg/g and the lowest in negative anthocyanin compounds. These secondary metabolite compounds have the potential as antibacterials against Aeromonas hydrophila bacteria.
Analysis of Changes In Biofloc Wastewater Quality Following Eco-Enzyme Application Nor Inayah; Noor Arida Fauzana; Fatmawati; Pahmi Ansyari
International Journal of Science and Environment (IJSE) Vol. 6 No. 2 (2026): May 2026
Publisher : CV. Inara in Colaboration with www.stie-sampit.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51601/ijse.v6i2.726

Abstract

The biofloc system improves water- and feed-use efficiency but generates wastewater containing suspended solids and nitrogenous compounds that require treatment before reuse. This study evaluated changes in biofloc wastewater quality after the addition of eco-enzyme at concentrations of 0% (control), 5%, 10%, 15%, 20%, and 25% (v/v). The wastewater and eco-enzyme were homogenized, incubated for 24 h, and analyzed for pH, dissolved oxygen (DO), ammonia (NH₃), total suspended solids (TSS), total nitrogen, total phosphorus, total potassium, and the C/N ratio. Data from two measurement replicates were analyzed using one-way analysis of variance followed by Fisher's least significant difference (LSD) test at the 5% significance level. Eco-enzyme had a highly significant effect on pH (p < 0.001), a significant effect on DO (p = 0.012), and a highly significant effect on NH₃ (p < 0.001). The pH increased from 2.760 in the control to 3.145 at the 25% dose, whereas DO decreased from 2.650 to 2.500 mg/L and NH₃ increased from 0.500 to 2.000 mg/L. TSS, total nitrogen, and total phosphorus did not differ significantly among treatments (p > 0.05). Total potassium increased from 0.0015% to 0.0705% (p < 0.001), while the C/N ratio increased from 0.460 to 6.310 (p = 0.004). These findings indicate that, after 24 h of contact, eco-enzyme functions more as a carbon source and a mineralization bioactivator than as a stand-alone treatment capable of directly producing water suitable for aquaculture. The treated water still requires neutralization, aeration, ammonia control, and a longer maturation period before reuse.
Co-Authors Noor Arida Fauzana Nor Inayah Pahmi Ansyari Siti Aisiah Siti Aminah