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All Journal Jurnal Aquatik Journal of Microbiology, Biotechnology and Conservation
Immaria Fransira
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology

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PENGARUH PERBEDAAN METODE PENGERINGAN TERHADAP KANDUNGAN METABOLIT PRIMER RUMPUT LAUT Kappaphycus alvarezii Waldetrudis Anunut; Ade Yulita Hesti Lukas; Immaria Fransira
Jurnal Aquatik Vol 9 No 1 (2026): Edisi Maret 2026
Publisher : Nusa Cendana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jaqu.v9i1.29953

Abstract

Kappaphycus alvarezii is one of the fishery commodities with high economic value, particularly as a source of carrageenan and primary metabolites. The drying process is a crucial post-harvest stage as it may affect the quality of nutritional content yield. This study aimed to determine the effect of different drying methods on the primary metabolite content of Kappaphycus alvarezii seaweed. The study was designed using a Completely Randomized Design (CRD) with three treatments and three replications, consisting of outdoor hanging drying (P1), solar dome dryer (P2), and outdoor rack drying (P3). The observed parameters included protein, fat, carbohydrate, and carrageenan content, which were analyzed using Analysis of Variance (ANOVA). The results showed that the different drying methods had no significant effect (P > 0.05) on all parameters tested. The highest average protein content was obtained in treatment P1 at 6.23%, the highest fat content in P3 at 1.06%, the highest carbohydrate content in P1 at 40.90%. All three drying methods were able to maintain the nutritional content of the seaweed within a relatively comparable range, suggesting that traditional drying methods can serve as an effective and efficient alternative for seaweed farmers. Keywords: Carrageenan, Kappaphycus alvarezii, Primary metabolites, Drying method, Seaweed.
IDENTIFIKASI JENIS BAKTERI PADA RUMPUT LAUT Eucheuma cottonii YANG TERSERANG ICE-ICE Rosalia Adedelcy Fernandez; Yuliana Salosso; Immaria Fransira
Jurnal Aquatik Vol 9 No 1 (2026): Edisi Maret 2026
Publisher : Nusa Cendana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jaqu.v9i1.30063

Abstract

Ice-ice disease is one of the main obstacles in the cultivation of Eucheuma cottonii seaweed which can reduce the growth, quality, and carrageenan content. This study aims to identify the types of bacteria found in E. cottonii seaweed infected with ice-ice disease and determine its effect on carrageenan levels. The study was conducted in August–October 2025 with samples taken at Pasir Panjang Beach, Kupang City. Bacterial identification was carried out at the Fish and Environmental Health Laboratory UPT using the API 20 E method, while carrageenan analysis was carried out using the alkali extraction method. Water quality parameters measured included temperature, salinity, pH, and dissolved oxygen (DO). The results showed that the bacteria identified in the seaweed samples were Vibrio alginolyticus, while no pathogenic bacteria were found in the water samples. Healthy seaweed produced carrageenan yields of 28.0% and 32.0%, while seaweed infected with ice-ice only produced yields of 17.0% and 12.0%. Water quality measurements showed a temperature of 32.8°C, a salinity of 30 ppt, a pH of 8.1, and a DO of 7.50 ppm. The results showed that the presence of Vibrio alginolyticus is associated with ice-ice disease in E. cottonii and results in decreased carrageenan levels. Environmental conditions, particularly relatively high temperatures, are thought to increase the seaweed's susceptibility to bacterial infection. Key words: Eucheuma cottonii, ice-ice disease, Vibrio alginolyticus, carrageenan, water quality.
Effect of Feeding Frequency of Ulva lactuca-Supplemented Diet on Growth Performance and Feed Efficiency of Nile Tilapia (Oreochromis niloticus) Chrisna Gidalti Upu; Ridwan Tobuku; Immaria Fransira
Journal of Microbiology, Biotechnology and Conservation Vol. 2 No. 2 (2026): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/tabhb544

Abstract

This study aimed to evaluate the effect of feeding frequency of a diet supplemented with 12% Ulva lactuca meal on the growth performance, feed efficiency, and survival rate of Nile tilapia (Oreochromis niloticus). The experiment was conducted for 60 days at the Noekele Fish Hatchery Center, Kupang Regency, Indonesia. A Completely Randomized Design (CRD) was employed with three feeding frequency treatments and three replicates: P1 (feeding twice daily), P2 (feeding three times daily), and P3 (feeding four times daily). Parameters observed included weight gain, specific growth rate (SGR), feed efficiency, survival rate, and water quality. Data were analyzed using one-way analysis of variance (ANOVA) followed by the Least Significant Difference (LSD) test at a 95% confidence level. The results showed that feeding frequency significantly affected growth performance, feed efficiency, and survival rate of Nile tilapia (P<0.05). The highest performance was obtained in treatment P3, with a weight gain of 7.72 ± 0.77 g, specific growth rate of 2.41 ± 0.15% day⁻¹, feed efficiency of 76.66 ± 5.58%, and survival rate of 100%. These values were significantly higher than those observed in P1 and P2. Increased feeding frequency likely improved nutrient availability and utilization, resulting in enhanced growth and feed conversion efficiency. Water quality parameters remained within the optimal range for Nile tilapia culture, with temperatures of 26–30°C and pH values of 7.0–8.5 throughout the study. In conclusion, feeding Nile tilapia with a diet containing 12% Ulva lactuca meal four times per day is the optimal feeding frequency to improve growth performance, feed efficiency, and survival rate
Co-Authors Ade Yulita Hesti Lukas Chrisna Gidalti Upu Ridwan Tobuku Rosalia Adedelcy Fernandez Waldetrudis Anunut Yuliana Salosso