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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement)
Adib Fakhruddin Yusuf
Laboratory of Genetic and Breeding, Faculty of Biology, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Immunology & microbiology Veterinary

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INTRA-SPESIFIC DIVERSITY OF BUTTERFLY PEA (Clitoria ternatea L.) REVEALED BY ISSR WITH INVARIABLE ITS RECORDS Adib Fakhruddin Yusuf; Vida Rahma Latifah; Vivi Indah Nurcahyati; Anggun Diyan Nurhasanah; Adristi Shafa Widyasari; Ananto Puradi Nainggolan; Aldy Riau Wansyah Hasibuan; Madyan Akmal Hidayat; Karmilah Karmilah; Arini Dian Pratiwi; Rindu Aurantika; Muslifah Hasanah; Ganies Riza Aristya; Niken Satuti Nur Handayani; Tuty Arisuryanti; Indra Lesmana; Budi Setiadi Daryono
BIOTROPIA Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.1.2362

Abstract

Article Highlights:- The ITS region exhibits no genetic variation among butterfly pea genotypes.- ISSR reveals high polymorphism, proving effective for genetic diversity analysis.- White single and purple single genotypes show the greatest genetic distance.- ISSR-6 ((CT)₈TG) demonstrates the highest polymorphism and reliability for assessment.- The ITS sequences have been deposited in GenBank under the accession IDs PQ198055–PQ198061. ABSTRACTClitoria ternatea L., a perennial plant in the Fabaceae, is recognized for its resilience in tropical climates and its diverse applications in both culinary and medicinal fields. However, the limited exploration of its genetic diversity constrains breeding efforts aimed at improving desirable traits. This limitation highlights the need to optimize selection strategies, identify superior genotypes, and preserve valuable genetic resources for long-term conservation and crop enhancement. This study aimed to explore genetic variation using molecular markers to analyze C. ternatea genotypes based on petal architecture and color differences. To assess the genetic diversity of C. ternatea, Sanger sequencing of the Internal Transcribed Spacer (ITS) region and Inter-Simple Sequence Repeat (ISSR) markers were applied to seven wild populations from Sleman, Yogyakarta. The ITS region exhibited no genetic variation, indicating its conserved nature and limited ability to differentiate genotypes. In contrast, ISSR markers effectively detected genetic variation, identifying 62 polymorphic fragments out of 162 total bands. The highest genetic distance (0.297) was observed between the WS and PS genotypes, whereas the double-petal genotypes (WD and PD) displayed the closest phenetic relationship. Among the ISSR primers, UBC-808, UBC-812, and ISSR-6 exhibited high PIC and RP values, confirming their reliability in genetic diversity analysis. These results underscore the utility of ISSR markers as a robust tool for genetic diversity assessment, offering valuable insights for breeding programs and germplasm conservation in C. ternatea.
Initiating Sustainable Agriculture through Local ‘Sleman’ Longan Cultivation and Stingless Beekeeping in the Sinduadi Farmers Group Adib Fakhruddin Yusuf; Dian Sartika; Vida Rahma Latifah; Karso Suryo Putro; Novia Noor Rachmawati; Prima Sekti Kusnanda; Tiara Amelia Putri; Budi Setiadi Daryono; Tri Joko; Ignatius Sudaryadi
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 12, No 1 (2026): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.97885

Abstract

Sinduadi Village, located in Mlati Subdistrict, Sleman Regency, Special Region of Yogyakarta, Indonesia, is home to several active horticultural farmer groups. Among their commodity crops, longan cultivation in Sinduadi Village has faced persistent constraints, particularly low productivity. To address this limitation, a community service program was initiated to introduce a superior local variety, the ‘Sleman’ longan. This cultivar was developed through sustained research and breeding by the Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada (UGM). Stingless bees, locally known as klanceng bees, play an important role as pollinators in horticultural systems. Integrating longan cultivation with stingless beekeeping as a managed pollination strategy is expected to improve longan productivity and support more sustainable farm management. The program applied a comprehensive capacity building approach, including lectures, extension sessions, hands-on training, and regular monitoring. These activities aimed to strengthen both conceptual understanding and practical competence related to cultivation of the ‘Sleman’ longan variety and stingless beekeeping management. The program resulted in an observable improvement in participants’ knowledge and skills within the Sinduadi Village farming group. Improvements in farmer capacity will be evaluated through continued monitoring of field practices and performance over time. Although short-term gains in yield have not yet been observed, substantial longer-term benefits are anticipated as the plants establish, adapt, and enter stable production. Overall, this knowledge transfer is expected to empower the community to address local agricultural constraints more independently and to promote sustained improvements in livelihood and well-being.
Co-Authors Adristi Shafa Widyasari Aldy Riau Wansyah Hasibuan Ananto Puradi Nainggolan Anggun Diyan Nurhasanah Arini Dian Pratiwi Budi Setiadi Daryono Budi Setiadi Daryono Dian Sartika Ganies Riza Aristya Ignatius Sudaryadi Indra Lesmana Karmilah Karmilah Karso Suryo Putro Madyan Akmal Hidayat Muslifah Hasanah Niken Satuti Nur Handayani Novia Noor Rachmawati Prima Sekti Kusnanda Rindu Aurantika Tiara Amelia Putri Tri Joko Tuty Arisuryanti Vida Rahma Latifah Vida Rahma Latifah Vivi Indah Nurcahyati