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Anggi, Viani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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LC-HRMS Analysis of Abelmoschus Manihot Medik from Palu of Central Sulawesi Paliwang, Rafni Mahyudin; Anggi, Viani; Yanuarty, Rezky; Rakanita, Yasinta
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.8948

Abstract

Abelmoschus manihot (L.) Medik is one of the traditional medicinal plants from Palu City, Central of Sulawesi with the Malvaceae plant family and commonly is a traditional medicinal plants recognized fot its therapeutic potential, particularly in antioxidant, anti-inflammatory and wound healing applications. This research aims to identify and characterize the chemical compounds present in Abelmoschus manihot (L.) Medik plant from Palu City, Central of Sulawesi using  liquid chromatography high resolution mass spectrometry (LC-HRMS). This research was collected fresh plant, designed using ethanol extraction from Abelmoschus manihot (L.) Medik and analyzed using mass spectroscopy (LC-HRMS) with Processing Software – MZMine Ver. 3.9.0. The results of this study  provide new insights into the chemical composition of Abelmoschus manihot (L.) Medik and confirm its potential as a source of bioactive compounds that could be futher explored for pharmaceutical development, namely that it contains the main compound Quercetin with a mass of 303.04, Gossypetin; Myricetin with a mass of 319.04, Quercetin 3-O-alpha-L-rhamnoside with a mass of 449.10 Isoquercetin; Hyperoside; Hyperin; Hirsutrin with a mass of 465.10, Gossypol with a mass of 519.20 and rutin with a mass of 291.08.
Test of the Effectiveness of Earthworm Flour (Lumbricus rubellus) Gastroretentive Mucoadhesive Granule Formulation on Male White Rats (Rattus norvegicus) Infected with Salmonella typhi Magfirah; Bengkati, Stely Gracia; Tandi, Joni; Anggi, Viani
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.8953

Abstract

Lumbricus rubellus contains lumbricin which is efficacious for treating typhoid fever. The aim of the research was to determine the effectiveness of gastroretentive mucoadhesive granule preparations from earthworm flour. This study used 30 male Wistar rats which were divided into 6 groups which were given orally, the normal and negative control groups were given Na-CMC, the positive control group was given chloramphenicol, and the treatment group was given doses of 200 mg/kgBW, 400 mg/kgBW and 800 mg/kgBW. mg/kgBB gastroretentive mucoadhesive granules earthworm flour. The results of body temperature measurements, on day 7 the control group obtained results of ±37.08, ±39.00 and ±38.09 respectively, and the treatment group obtained results of ±38.09, ±38.09 and ±38.07 respectively. On day 21, the control group obtained results of ±37.08, ±39.00 and ±38.09, respectively, and the treatment group obtained results of ±38.09, ±38.09 and ±38.07, respectively. The Widal test results on the 7th day were positive and after 14 days of administering the preparation on the 21st day the results were negative. It was concluded that the effective dose for typhoid fever therapy was a dose of 400 mg/kgBW.
Co-Authors Bengkati, Stely Gracia Joni Tandi, Joni Magfirah Paliwang, Rafni Mahyudin Rakanita, Yasinta Yanuarty, Rezky