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All Journal Jurnal Biologi Tropis Bioscientist : Jurnal Ilmiah Biologi
Nazwa S. Lamante
Universitas Negeri Gorontalo
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Identifikasi Tahap Perkembangan Mikrospora melalui Ukuran Floret pada Jantung Pisang Tanduk Devi Bunga Pagalla; Jusna Ahmad; Indriati Husain; Nazwa S. Lamante; Rika Eyato
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.17681

Abstract

This study aimed to identify the relationship between floret length and the developmental stages of microspores in banana male inflorescence (Musa spp. cv. Tanduk), and to determine the optimal floret size range as an explant source for initiating microspore culture. Ten bract layers were analyzed, with five florets randomly selected from each layer and measured for their length. Anthers were extracted from each floret, crushed in sterile water, and observed microscopically using a wet mount preparation. The results revealed a strong correlation between floret length and microspore developmental stages. Microspores at the late uninucleate (vacuolated) to early bicellular stages were consistently found in florets ranging from 4.68 to 4.34 cm in length. Based on these findings, florets within the 4.60 to 4.00 cm range are recommended as the optimal size for microspore culture initiation, as they contain a higher proportion of developmentally competent microspores for embryogenesis induction. In conclusion, this study provides essential morphological and cytological criteria to support efficient explant selection for in vitro regeneration of banana through the microspore culture approach.
Influence of Various Types of Chitosan on Biomass and Callus Morpology of Momordica Cochinchinensis in Vitro Nazwa S. Lamante; Devi Bunga Pagalla; Jusna Ahmad; Novri Youla Kandowangko; Indriati Husain
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): Juli - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12314

Abstract

Momordica cochinchinensis is a medicinal, herbs locally known in gorontallo as dumbaya. Tradisionally, this plant has been utilized as both a food of food course a herbal medicine. Due to its rich contains of bioactive compounds, making it a potential candidate for development through plant tissue culture. This study aimed to determine the effects of different chitosan concentrations on the biomass and callus morphology of M. cochinchinensis cultivated in vitro. The experiment was arranged in a non-factorial completey randomized design consisting of three chitosan concentrations: 15 mg L -1, 25 mg L-1, and 35 mg L-1, respectively, as well as a 5000 mg L-1 chitosan stock solution per liter of MS medium. Each treatment consisted of three replicates. The observed parameters included callus fresh weight, dry weight, color, texture, and the percentage of callus formation. Data were analyzed using a one-way ANOVA at a 5% significance level. Since the fresh weight data showed significant differences, it was followed by a Duncan’s Multiple Range Test (DMRT) at the 5% level. The results showed that different chitosan concentrations significantly affected the callus fresh weight (F = 7.932; p = 0.021). the 15 mg L-1 treatment yielded the highest fresh weight, 0.04067 ± 0.0115 g, which was significantly different from the 35 mg L-1 treatment (0.3000 ± 0.0458 g) but not significantly different from the 25 mg L-1 treatment (0.3300 ± 0.0346 g) conversely, chitosan concentration did not significantly affect the culls dry weight (F = 3.519); p = 0.097). all treatmeants resulted in 100% callus formation with white and friable callus morphology. These findings indicate that among the tasted concentrations, 15 mg L-1 of chitosan is the most favorable concentration for demonstrating callus fresh weight accumulation of M. cochinchinensis under in vitro culture conditions.
Co-Authors Devi Bunga Pagalla Indriati Husain Jusna Ahmad Novri Youla Kandowangko Rika Eyato