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All Journal Journal of Tropical Biodiversity and Biotechnology BIOEDUSCIENCE Jurnal Biologi Tropis Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Jurnal Ilmiah Biologi UMA (JIBIOMA) Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Hydrogen: Jurnal Kependidikan Kimia BIOVALENTIA: Biological Research Journal Lamahu: Jurnal Pengabdian Masyarakat Terintegrasi JURNAL SIPISSANGNGI: Jurnal Pengabdian Kepada Masyarakat Journal of Applied Community Engagement (JACE) Jurnal Abdimas Terapan Biodik: Jurnal Ilmiah Pendidikan Biologi Science: Indonesian Journal of Science Damhil: Jurnal Pengabdian kepada Masyarakat Bioma : Jurnal Biologi dan Pembelajaran Biologi
Devi Bunga Pagalla
Program Studi Biologi, Jurusan Biologi, FMIPA, Universitas Negeri Gorontalo, Jl. Jendral Sudirman No. 6 Kota Gorontalo - Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Induction of Microspore Embryogenesis of Eggplant (Solanum melongena L.) ‘Gelatik’ Devi Bunga Pagalla; Ari Indrianto; Maryani Maryani; Endang Semiarti
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 2 (2020): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.53677

Abstract

The haploid or double haploid plant of eggplants could be produced from microspore culture (embryogenesis of microspores). In the breeding programs, microspore can be developed into an embryo directly after exposure to stress treatment during cultured. Stress (temperature and starvation medium) is an important factor in the induction of embryogenesis microspore. This study aims to induced embryogenic microspores from eggplant CV. Gelatik. The stage late-uninucleate microspore (Vacuolate Microspore/VM) and early binucleate (Young Bicellular Pollen/YBP) are the suitable stages to induce multinucleate structure. There are 3 methods used in this research; 1) Determination of the stage development of microspore based on flower buds length and anther length. 2) Induction of embryogenic microspore on the pre-treatment and starvation medium. 3) After giving pre-treatment for 4 days, micropores were transferred to culture medium A2 at 28oC in dark conditions to induce the multicellular structures. This study reported that 50-68.51% of the VM+YBP stage obtained in the range of flower bud lengths of 10-17 mm, and 5.0-6.9 mm, the range of anther length containing VM+YBP of 50-77.48%. The pre-treatment heat shock at 33oC in the medium B for 2 days,  produced embryogenic microspores with a high percentage, that is about 50.19%, while microspores at 25oC and 4oC respectively 46.17% and 49.28%. Pre-treatment for 4 days at 4 oC, 25 oC,  and 33oC with the percentage of embryogenic microspores apiece 32.87%, 27.45%, and 37.34%. The multicellular (starlike) structure begins forming on the fifth day of incubation in culture medium (A2) after pre-treatment in B medium at 33oC.
Pengenalan Zat Aditif dan Adiktif yang Berbahaya Bagi Kesehatan di Lingkungan MTs. Negeri 2 Kabupaten Gorontalo Herinda Mardin; Hartono D Mamu; Nurul Fajryani Usman; Nur Mustaqimah; Devi Bunga Pagalla
Lamahu: Jurnal Pengabdian Masyarakat Terintegrasi Vol 1, No 2: August 2022
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (605.138 KB) | DOI: 10.34312/ljpmt.v1i2.15466

Abstract

The introduction of additives and addictive substances for the younger generation, especially the millennial generation, is very important because children's habits, especially when they are teenagers, do snack activities at school and the surrounding environment without having a selective attitude for the sake of health. The habit of snacking and the lack of knowledge and understanding of healthy snacks and the content of foods that are harmful to health make students not have a selective attitude in consuming snacks in the school environment. Additives contained in food, if excessive can have a negative effect on health. Likewise with addictive substances if misused it will cause negative effects for the body. The purpose of this service activity is to introduce additives and addictive substances that are harmful to health for students aged children and adolescents so that they are able to prevent disease, create a selective attitude in consuming food for the sake of health and save our children from abuse of harmful addictive substances. . The method used in this service activity is the lecture method with a participatory approach through 4 stages of activity, namely, opening, giving material, question and answer/discussion and closing. The socialization activity went well, this could be seen from the enthusiasm of the participants in participating in the socialization activity from beginning to end and actively asking questions and discussing. Learners know the types of additives and addictive substances that are harmful to health and know how to prevent abuse of dangerous addictive substances. In addition, it also adds insight and knowledge to participants about additives and addictive substances that are harmful to health so that students have a selective attitude in snacking activities and consuming food and drinks in the school environment and its surroundings.
Tahap Perkembangan Mikrospora pada Solanum melongena L. Devi Bunga Pagalla
BIOEDUSCIENCE Vol 7 No 1 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/7111357

Abstract

Background: Microspores are small haploid spores that develop into the male gametophyte. Microsporocytes undergo meiotic division to form microspores. Microspores can be found in seedless and seed plants. The microspores in each flowering plant are different. This study aims to observe microspores on eggplant flowers. Method: Microspore observations were carried out on different flower bud sizes until the flower buds bloomed. Result: The results showed microspores in eggplant had different stages of development for each flower bud size. The stages of microspore development observed were Early uninucleate (Young microspore), Mid-uninucleate, Late uninucleate (Vacuolate microspore), early binucleate (Young bicellular pollen), mid-binucleate, and mature pollen. Conclusion: In eggplant microspore culture, anther length is a strong parameter to predict the stage of microspore development contained there in.
Pengukuran Aktivitas Nitrat Reduktase (ANR) Pada Tanaman Poaceae Secara In vivo Devi Bunga Pagalla; Magfirahtul Jannah
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 5, No 1 (2023): Mei 2023
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v5i1.1681

Abstract

Corn, rice, and sugarcane are included in the Poaceae family which have high economic value as staple foods. Nitrate reductase plays a role in reducing nitrate to ammonia. The nitrate reductase activity testing can help biotechnology studies, in this case, fertilizer production or organic waste treatment, because the nitrate reductase enzyme can be used to convert nitrate into more useful nitrogen compounds. The purpose of this study was to analyze the activity of nitrate reductase (ANR) from the leaves of the Poaceae family, namely corn, rice, and sugarcane. In vivo ANR measurements were carried out using the spectrophotometric method with two types of spectrophotometers, namely the Vis/UV Spectrophotometer, Genesys10UV, and Visible Spectrophotometer, with a wavelength of 540 nm. This study concludes that rice had the highest ANR of 0.1503 µmol/gram leaf weight/hour of incubation. The increase in nitrate reductase activity indicates that the energy for nitrate reduction also increases and provides a large capacity for synthesizing amino acids, proteins, or total N assimilation.
PENGEMBANGAN PRODUK BERBAHAN DASAR IKAN SEBAGAI UPAYA PEMANFAATAN HASIL TANGKAPAN Devi Bunga Pagalla; Nur Mustaqimah; Magfirahtul Jannah
Jurnal Abdimas Terapan Vol. 2 No. 2 (2023): JURNAL ABDIMAS TERAPAN (MEI)
Publisher : Program Vokasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.336 KB) | DOI: 10.56190/jat.v2i2.27

Abstract

Kecamatan Bonepantai merupakan salah satu kawasan pengembangan perikanan (KPP 4). Kecamatan Bonepantai memiliki 8 desa nelayan, salah satunya Desa Bilungala. Secara keseluruhan Kecamatan Bonepantai memiliki jumlah nelayan ataupun RTP tertinggi kedua setelah Kecamatan Kabila Bone. Melihat potensi Kelautan dan Perikanan yang cukup besar di Kecamatan Bonepantai, perlu dilakukan usaha strategis untuk pengembangan produk perikanan sebagai upaya pemanfaatan hasil tangkapan laut. Pengolahan ikan dikalangan masyarakat desa bilungala masih sebatas olahan bakar, abon, dan acar ikan. Kegiatan ini bertujuan memberdayakan masyarakat khususnya Ibu-Ibu dengan melakukan pelatihan pembuatan nugget berbahan dasar Ikan Cakalang. Metode yang digunakan adalah metode partisipatif dimana masyarakat ditempatkan sebagai subjek dalam setiap tahapan kegiatan. Kegiatan pelatihan pembuatan nugget dilaksanakan dengan jumlah peserta 15 orang Ibu-ibu Kader Posyandu Desa Bilungala. Kegiatan ini diawali dengan presentasi mengenai bahan baku yang dapat digunakan dalam pembuatan nugget dan penjelasan singkat terkait cara pembuatan dan penyimpanan yang baik dan benar. Melalui kegiatan pelatihan pembuatan nugget ikan ini diharapkan masyarakat Desa Bilungala mampu mengolah hasil tangkapan menjadi produk olahan dengan maksimal untuk menunjang kesejahteraan dan memilih nugget ikan sebagai cemilan sehat bagi anak-anak di Desa Bilungala
In Vitro Germination of Dumbaya Seeds (Momordica cochinchinensis (Lour.) Spreng: A Unique Medicinal Plant of Gorontalo Devi Bunga Pagalla; Jusna Ahmad; Miftahul F. Adudu; Adilah Nidaulhasanah; Fitria H.Ys. Adju; Evita Moni Damayanti
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.5798

Abstract

"Dumbaya (Momordica cochinchinensis (Lour.) Spreng) is one of the plant species believed by the Gorontalo community to be a traditional remedy for various ailments such as inflammation, liver disorders, spleen issues, hemorrhoids, bruising, and infected wounds. Currently, the dumbaya plant is difficult to find and is even considered nearly extinct by the Gorontalo community. This is due to the challenging conventional cultivation of dumbaya plants from seeds. Dumbaya seeds have a hard, stone-like texture, making germination in the wild difficult and requiring special treatment. Regeneration of dumbaya can be achieved through tissue culture techniques, replacing the conventional cultivation system.Through tissue culture, dumbaya seeds are grown in specialized planting media and controlled environments. Based on observations, the propagation of dumbaya plants using in vitro culture techniques has proven successful and effective as a solution for propagating dumbaya seedlings. Dumbaya seeds begin to germinate two weeks after planting (2 WAP). The grown dumbaya seedlings can then be used as explants for further dumbaya propagation by inducing callus formation."
Tahap Perkembangan Mikrospora pada Solanum melongena L. Devi Bunga Pagalla
BIOEDUSCIENCE Vol 7 No 1 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/7111357

Abstract

Background: Microspores are small haploid spores that develop into the male gametophyte. Microsporocytes undergo meiotic division to form microspores. Microspores can be found in seedless and seed plants. The microspores in each flowering plant are different. This study aims to observe microspores on eggplant flowers. Method: Microspore observations were carried out on different flower bud sizes until the flower buds bloomed. Result: The results showed microspores in eggplant had different stages of development for each flower bud size. The stages of microspore development observed were Early uninucleate (Young microspore), Mid-uninucleate, Late uninucleate (Vacuolate microspore), early binucleate (Young bicellular pollen), mid-binucleate, and mature pollen. Conclusion: In eggplant microspore culture, anther length is a strong parameter to predict the stage of microspore development contained there in.
Pemanfaatan Hasil Tangkapan Ikan Dengan Pelatihan Pembuatan Sossis Ikan Di Desa Bilungala Kecamatan Bonepantai Kabupaten Bone Bolango Nur Mustaqimah; Nurul Fajryani Usman; Devi Bunga Pagalla; Nurhayati Nurhayati
Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Vol 12, No 3 (2023): Jurnal Sibermas (Sinergi Bersama Masyarakat)
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/sibermas.v12i3.17910

Abstract

One of the areas in Gorontalo Province that has fishery potential from Tomini Bay is Bilungala Village, where the most commonly caught fish is Cakalang (Katsuwonus pelamis). Cakalang has a fairly high economic value and is much preferred because it has good meat density, especially to be used as a source of protein. But so far, the community does not have much knowledge about the management of Cakalang, which is the main catch. One of the processed products that can be made with Cakalang is fish sausage. Sausage is a food product that has nutritional value and meets the needs of the body. This processed product is also more practical to serve. The resulting product can also be an alternative business to improve the community's economy. Therefore, a fish processing training activity was carried out in the form of cakalang fish sausage. The aim of this training is to equip housewives in the village with skills in processing fish catches into nutritious and durable food products. The location of this community service activity was carried out in Bilungala Village, Bonepantai District, Bone Bolango Regency. The target is housewives in the village. The activity was carried out on November  which consisted of the preparation stage by conducting a survey and the implementation stage by conducting training with direct demonstrations and together with the participants making the product. This activity went smoothly. Participants were very enthusiastic about participating in the activity from the beginning of the activity to the end of the activity. This activity requires follow-up in the form of marketing training for processed fish products in the form of fish sausage. The training can be an asset for participants consisting of housewives to market their products which in the end are able to increase their income and help the community's economy.
PENGENALAN PRAKTIK SEDERHANA TENTANG TUMBUHAN KEPADA GURU-GURU IPA DI KOTA GORONTALO: MENCIPTAKAN PEMBELAJARAN YANG MENYENANGKAN BAGI SISWA SMP Devi Bunga Pagalla; Chairunnisah J. Lamangantjo; Masrid Pikoli; Jusna Ahmad; Najmah Najmah
Jurnal Abdimas Terapan Vol. 3 No. 2 (2024): JURNAL ABDIMAS TERAPAN (MEI)
Publisher : Program Vokasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56190/jat.v3i2.51

Abstract

Laboratorium Botani adalah fasilitas yang dilengkapi dengan alat dan bahan untuk mempelajari berbagai aspek tumbuhan seperti melakukan kajian anatomi tumbuhan, taksonomi tumbuhan, morfologi tumbuhan, dan eksperimen dalam bidang fisiologi tumbuhan. Di Laboratorium Botani, guru dan siswa dapat melakukan berbagai eksperimen sederhana yang menyenangkan tentang tumbuhan. Kegiatan ini bertujuan untuk memperkenalkan praktik sederhana di laboratorium botani yang dapat diterapkan oleh guru-guru IPA di Kota Gorontalo untuk membuat pembelajaran tentang dunia tumbuhan menjadi lebih kreatif dan menyenangkan bagi siswa. Metode pelaksanaan kegiatan meliputi ceramah untuk memberikan pemahaman dasar, diskusi interaktif untuk berbagi pengalaman dan menjawab pertanyaan, serta demonstrasi praktik melalui video untuk memvisualisasikan teknik-teknik sederhana yang dapat diaplikasikan dalam pengajaran. Hasil kegiatan menunjukkan peningkatan keterampilan dan pemahaman guru-guru IPA dalam menerapkan praktik laboratorium botani sederhana. Guru-guru mampu mengintegrasikan teknik-teknik tersebut ke dalam proses pembelajaran, yang berdampak positif pada minat dan partisipasi siswa dalam belajar tentang tumbuhan. Kegiatan ini berhasil mensosialisasikan praktik laboratorium botani yang sederhana namun efektif, yang dapat digunakan oleh guru-guru IPA untuk menciptakan pembelajaran yang lebih kreatif dan menyenangkan. Peningkatan keterampilan guru dan antusiasme siswa terhadap pembelajaran botani menjadi indikasi keberhasilan program ini, yang diharapkan dapat terus dikembangkan untuk meningkatkan kualitas pendidikan sains di Kota Gorontalo.
Introduction of Herbarium and Identification of Plants Based on School Environments in MTs. Negeri 2 Gorontalo Regency D. Mamu, Hartono; Mardin, Herinda; Bunga Pagalla, Devi; Mustaqimah, Nur; Fajryani Usman, Nurul
Journal of Applied Community Engagement Vol 2 No 1 (2022): Journal of Applied Community Engagement (JACE)
Publisher : ISAS (Indonesian Society of Applied Science)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.585 KB) | DOI: 10.52158/jace.v2i1.343

Abstract

The introduction of herbariums in schools as a medium to concrete material and support science learning and plant identification based on a school environment that makes it easier to teach material on the structure and function of plants in class VIII at Madrasah Tsanawiyah Negeri 2 Gorontalo district is the goal in this service activity. The stages of activities using an active participatory approach consist of 4 stages, namely 1) preparation; 2) coordination; 3) implementation; 4) evaluation. The number of students who took part in the activity was 30 people and was attended by the principal and 3 teachers. The herbarium introduction and plant identification activities based on the school environment went smoothly and wisely, as seen from the enthusiasm of the participants in participating in the activity from start to finish. The introduction of the herbarium gives meaning and impression to MTs students. Negeri 2 Gorontalo district because it is able to provide knowledge to students about one method/media to determine plant taxon. Identification of plants based on the school environment provides knowledge and skills to MTs students. Negeri 2 Gorontalo district in supporting learning activities in schools regarding the introduction and description of plants.
Co-Authors Abubakar Sidik Katili Adilah Nidaulhasanah Adju, Fitria Y.Hs. Ahaya, Cindi Ahmad Hidayat Alfiani, St Hijrah Ari Indrianto Arviani Arviani Aulia Rahmadhani Chairunnisah J Lamangantjo D, Nurul Iqraini Endang Semiarti Evita Moni Damayanti febriyanti febriyanti Febriyanti, Febriyanti Fitria H.Ys. Adju Gubali, Hayati Ningsih Haerani Haerani Hartono D Mamu Hartono D. Mamu Herinda Mardin Indriati Husain Isima , Lindawaty Jusna Ahmad Kandowangko, Novri Youla Lilan Dama Magfirahtul Jannah Maryani Maryani Masra Latjompoh Masrid Pikoli Menanti, Menanti Miftahul F. Adudu Mustaqimah, Nur Najmah Najmah Najmah, Najmah Novri Youla Kandowangko Nur Mustaqimah Nurhayati Nurhayati Nurhayati Nurhayati Nursanti, Andi Nurul Fajryani Usman Putri, Restiyani Widia Ramadhani, Andi Indra Wulan Sari Ramlah Ramlah Ramlah Ramlah Sangkota, Vivi Dia Afrianti Tuada, Rasydah Nur Walangadi, Farhana Rahmatia