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All Journal Journal of Tropical Biodiversity and Biotechnology BIOEDUSCIENCE Jurnal Biologi Tropis Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Jurnal Ilmiah Biologi UMA (JIBIOMA) Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Hydrogen: Jurnal Kependidikan Kimia Bioscientist : Jurnal Ilmiah Biologi BIOVALENTIA: Biological Research Journal Lamahu: Jurnal Pengabdian Masyarakat Terintegrasi JURNAL SIPISSANGNGI: Jurnal Pengabdian Kepada Masyarakat Journal of Applied Community Engagement (JACE) Jurnal Abdimas Terapan Biodik: Jurnal Ilmiah Pendidikan Biologi Science: Indonesian Journal of Science Damhil: Jurnal Pengabdian kepada Masyarakat Bioma : Jurnal Biologi dan Pembelajaran Biologi
Devi Bunga Pagalla
Program Studi Biologi, Jurusan Biologi, FMIPA, Universitas Negeri Gorontalo, Jl. Jendral Sudirman No. 6 Kota Gorontalo - Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Induction of Microspore Embryogenesis of Eggplant (Solanum melongena L.) ‘Gelatik’ Devi Bunga Pagalla; Ari Indrianto; Maryani Maryani; Endang Semiarti
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 2 (2020): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.53677

Abstract

The haploid or double haploid plant of eggplants could be produced from microspore culture (embryogenesis of microspores). In the breeding programs, microspore can be developed into an embryo directly after exposure to stress treatment during cultured. Stress (temperature and starvation medium) is an important factor in the induction of embryogenesis microspore. This study aims to induced embryogenic microspores from eggplant CV. Gelatik. The stage late-uninucleate microspore (Vacuolate Microspore/VM) and early binucleate (Young Bicellular Pollen/YBP) are the suitable stages to induce multinucleate structure. There are 3 methods used in this research; 1) Determination of the stage development of microspore based on flower buds length and anther length. 2) Induction of embryogenic microspore on the pre-treatment and starvation medium. 3) After giving pre-treatment for 4 days, micropores were transferred to culture medium A2 at 28oC in dark conditions to induce the multicellular structures. This study reported that 50-68.51% of the VM+YBP stage obtained in the range of flower bud lengths of 10-17 mm, and 5.0-6.9 mm, the range of anther length containing VM+YBP of 50-77.48%. The pre-treatment heat shock at 33oC in the medium B for 2 days,  produced embryogenic microspores with a high percentage, that is about 50.19%, while microspores at 25oC and 4oC respectively 46.17% and 49.28%. Pre-treatment for 4 days at 4 oC, 25 oC,  and 33oC with the percentage of embryogenic microspores apiece 32.87%, 27.45%, and 37.34%. The multicellular (starlike) structure begins forming on the fifth day of incubation in culture medium (A2) after pre-treatment in B medium at 33oC.
Pengenalan Zat Aditif dan Adiktif yang Berbahaya Bagi Kesehatan di Lingkungan MTs. Negeri 2 Kabupaten Gorontalo Herinda Mardin; Hartono D Mamu; Nurul Fajryani Usman; Nur Mustaqimah; Devi Bunga Pagalla
Lamahu: Jurnal Pengabdian Masyarakat Terintegrasi Vol 1, No 2: August 2022
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (605.138 KB) | DOI: 10.34312/ljpmt.v1i2.15466

Abstract

The introduction of additives and addictive substances for the younger generation, especially the millennial generation, is very important because children's habits, especially when they are teenagers, do snack activities at school and the surrounding environment without having a selective attitude for the sake of health. The habit of snacking and the lack of knowledge and understanding of healthy snacks and the content of foods that are harmful to health make students not have a selective attitude in consuming snacks in the school environment. Additives contained in food, if excessive can have a negative effect on health. Likewise with addictive substances if misused it will cause negative effects for the body. The purpose of this service activity is to introduce additives and addictive substances that are harmful to health for students aged children and adolescents so that they are able to prevent disease, create a selective attitude in consuming food for the sake of health and save our children from abuse of harmful addictive substances. . The method used in this service activity is the lecture method with a participatory approach through 4 stages of activity, namely, opening, giving material, question and answer/discussion and closing. The socialization activity went well, this could be seen from the enthusiasm of the participants in participating in the socialization activity from beginning to end and actively asking questions and discussing. Learners know the types of additives and addictive substances that are harmful to health and know how to prevent abuse of dangerous addictive substances. In addition, it also adds insight and knowledge to participants about additives and addictive substances that are harmful to health so that students have a selective attitude in snacking activities and consuming food and drinks in the school environment and its surroundings.
Tahap Perkembangan Mikrospora pada Solanum melongena L. Devi Bunga Pagalla
BIOEDUSCIENCE Vol 7 No 1 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/7111357

Abstract

Background: Microspores are small haploid spores that develop into the male gametophyte. Microsporocytes undergo meiotic division to form microspores. Microspores can be found in seedless and seed plants. The microspores in each flowering plant are different. This study aims to observe microspores on eggplant flowers. Method: Microspore observations were carried out on different flower bud sizes until the flower buds bloomed. Result: The results showed microspores in eggplant had different stages of development for each flower bud size. The stages of microspore development observed were Early uninucleate (Young microspore), Mid-uninucleate, Late uninucleate (Vacuolate microspore), early binucleate (Young bicellular pollen), mid-binucleate, and mature pollen. Conclusion: In eggplant microspore culture, anther length is a strong parameter to predict the stage of microspore development contained there in.
Pengukuran Aktivitas Nitrat Reduktase (ANR) Pada Tanaman Poaceae Secara In vivo Devi Bunga Pagalla; Magfirahtul Jannah
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 5, No 1 (2023): Mei 2023
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v5i1.1681

Abstract

Corn, rice, and sugarcane are included in the Poaceae family which have high economic value as staple foods. Nitrate reductase plays a role in reducing nitrate to ammonia. The nitrate reductase activity testing can help biotechnology studies, in this case, fertilizer production or organic waste treatment, because the nitrate reductase enzyme can be used to convert nitrate into more useful nitrogen compounds. The purpose of this study was to analyze the activity of nitrate reductase (ANR) from the leaves of the Poaceae family, namely corn, rice, and sugarcane. In vivo ANR measurements were carried out using the spectrophotometric method with two types of spectrophotometers, namely the Vis/UV Spectrophotometer, Genesys10UV, and Visible Spectrophotometer, with a wavelength of 540 nm. This study concludes that rice had the highest ANR of 0.1503 µmol/gram leaf weight/hour of incubation. The increase in nitrate reductase activity indicates that the energy for nitrate reduction also increases and provides a large capacity for synthesizing amino acids, proteins, or total N assimilation.
In Vitro Germination of Dumbaya Seeds (Momordica cochinchinensis (Lour.) Spreng: A Unique Medicinal Plant of Gorontalo Devi Bunga Pagalla; Jusna Ahmad; Miftahul F. Adudu; Adilah Nidaulhasanah; Fitria H.Ys. Adju; Evita Moni Damayanti
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.5798

Abstract

"Dumbaya (Momordica cochinchinensis (Lour.) Spreng) is one of the plant species believed by the Gorontalo community to be a traditional remedy for various ailments such as inflammation, liver disorders, spleen issues, hemorrhoids, bruising, and infected wounds. Currently, the dumbaya plant is difficult to find and is even considered nearly extinct by the Gorontalo community. This is due to the challenging conventional cultivation of dumbaya plants from seeds. Dumbaya seeds have a hard, stone-like texture, making germination in the wild difficult and requiring special treatment. Regeneration of dumbaya can be achieved through tissue culture techniques, replacing the conventional cultivation system.Through tissue culture, dumbaya seeds are grown in specialized planting media and controlled environments. Based on observations, the propagation of dumbaya plants using in vitro culture techniques has proven successful and effective as a solution for propagating dumbaya seedlings. Dumbaya seeds begin to germinate two weeks after planting (2 WAP). The grown dumbaya seedlings can then be used as explants for further dumbaya propagation by inducing callus formation."
Tahap Perkembangan Mikrospora pada Solanum melongena L. Devi Bunga Pagalla
BIOEDUSCIENCE Vol 7 No 1 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/7111357

Abstract

Background: Microspores are small haploid spores that develop into the male gametophyte. Microsporocytes undergo meiotic division to form microspores. Microspores can be found in seedless and seed plants. The microspores in each flowering plant are different. This study aims to observe microspores on eggplant flowers. Method: Microspore observations were carried out on different flower bud sizes until the flower buds bloomed. Result: The results showed microspores in eggplant had different stages of development for each flower bud size. The stages of microspore development observed were Early uninucleate (Young microspore), Mid-uninucleate, Late uninucleate (Vacuolate microspore), early binucleate (Young bicellular pollen), mid-binucleate, and mature pollen. Conclusion: In eggplant microspore culture, anther length is a strong parameter to predict the stage of microspore development contained there in.
Introduction of Herbarium and Identification of Plants Based on School Environments in MTs. Negeri 2 Gorontalo Regency D. Mamu, Hartono; Mardin, Herinda; Bunga Pagalla, Devi; Mustaqimah, Nur; Fajryani Usman, Nurul
Journal of Applied Community Engagement Vol 2 No 1 (2022): Journal of Applied Community Engagement (JACE)
Publisher : ISAS (Indonesian Society of Applied Science)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.585 KB) | DOI: 10.52158/jace.v2i1.343

Abstract

The introduction of herbariums in schools as a medium to concrete material and support science learning and plant identification based on a school environment that makes it easier to teach material on the structure and function of plants in class VIII at Madrasah Tsanawiyah Negeri 2 Gorontalo district is the goal in this service activity. The stages of activities using an active participatory approach consist of 4 stages, namely 1) preparation; 2) coordination; 3) implementation; 4) evaluation. The number of students who took part in the activity was 30 people and was attended by the principal and 3 teachers. The herbarium introduction and plant identification activities based on the school environment went smoothly and wisely, as seen from the enthusiasm of the participants in participating in the activity from start to finish. The introduction of the herbarium gives meaning and impression to MTs students. Negeri 2 Gorontalo district because it is able to provide knowledge to students about one method/media to determine plant taxon. Identification of plants based on the school environment provides knowledge and skills to MTs students. Negeri 2 Gorontalo district in supporting learning activities in schools regarding the introduction and description of plants.
EDUKASI PEMANFAATAN POTENSI PANGAN LOKAL SULAWESI BARAT SEBAGAI SUMBER BELAJAR BIOLOGI DI MAN 1 POLEWALI MANDAR Ramlah, Ramlah; D, Nurul Iqraini; Alfiani, St Hijrah; Nursanti, Andi; Haerani, Haerani; Tuada, Rasydah Nur; Pagalla, Devi Bunga; Rahmadani, Aulia
JURNAL SIPISSANGNGI: Jurnal Pengabdian Kepada Masyarakat Vol 4, No 2 (2024): Sipissangngi Volume 4, Nomor 2, Juni 2024
Publisher : Lembaga Penelitan dan Pengabdian Masyarakat, Universitas Al Asyariah Mandar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35329/jurnal.v4i2.5049

Abstract

Pangan lokal adalah pangan yang diproduksi dalam jarak dekat dari tempat konsumsinya, dan dibudidaya oleh masyarakat setempat. Gerakan pangan lokal bertujuan untuk menghubungkan produsen (petani lokal) dan konsumen pangan yang berada pada wilayah geografis terbatas atau berada pada wilayah yang sama, termasuk pangan lokal yang ada di Provinsi Sulawesi Barat. Tujuan kegiatan ini yaitu untuk memberikan edukasi pemanfaatan pangan lokal sebagai sumber belajar biologi bagi siswa-siswi MAN 1 Polewali Mandar. Metode kegiatan pengabdian kepada masyarakat (PKM) menggunakan metode edukasi terkait pemanfaatan pangan lokal menjadi pangan potensial. Kegiatan ini dihadiri siswa-siswi kelas XI MAN 1 Polewali Mandar yang berasal dari jurusan IPA, IPS, Bahasa, dan Agama. Kegiatan pengabdian ini dilakukan dengan mengedukasi potensi pangan lokal, manfaatnya bagi kesehatan, dan lingkungan, serta mengenalkan jenis-jenis produk olahan pangan lokal di Sulawesi Barat. Selanjutnya, dengan mengikutsertakan seluruh peserta dalam pemberian contoh produk olahan pangan potensial. Selain itu, produk pangan lokal dapat diolah menjadi produk Usaha Mikro Kecil dan Menengah (UMKM) sehingga dapat meningkatkan kesejahteraan masyarakat lokal. Hasil evaluasi diperoleh bahwa kegiatan PKM memberikan pemahaman baru yang menarik kepada peserta terkait potensi pangan lokal. Sebanyak 81,26% peserta menyatakan sangat setuju terhadap kegiatan edukasi yang telah dilaksanakan. Sebanyak 89,7% peserta juga mengungkapkan bahwa kegiatan pengabdian menambah pemahaman peserta terkait manfaat pangan lokal bagi kesehatan, lingkungan, dan masyarakat. Selain itu, sebanyak 83,67% peserta mengungkapkan bahwa kegiatan PKM yang dilakukan dapat dijadikan sebagai sumber belajar biologi bagi siswa-siswi yang ada di MAN 1 Polewali Mandar. 
Sosialisasi Pemanfaatan Ikan Laut sebagai Sumber Pangan Kaya Nutrisi di Desa Bilungala, Kecamatan Bonepantai, Kabupaten Bone Bolango Nurul Fajryani Usman; Devi Bunga Pagalla; Magfirahtul Jannah; Nurhayati Nurhayati
Damhil: Jurnal Pengabdian kepada Masyarakat Vol 2, No 1: June 2023
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34312/damhil.v2i1.17811

Abstract

Gorontalo Province is one of the provinces that has a wealth of natural resources, especially fishery and marine products. One area that has the highest fish production is Bilungala Village, Bone Bolango Regency. Fish is a food rich in protein, minerals, vitamins, and fatty acids. In addition, fish also contain omega-3 fatty acids and vitamin K which the body needs. All the nutritional content in fish is very influential on the growth and development of children. But in fact, the level of fish consumption in Indonesia is still very low compared to other largest fish-producing countries. Therefore, socialization is carried out through the use of marine fish as a source of nutrient-rich food. The purpose of this service is to provide information to the public about the nutritional content of fish, increase fish consumption in the community, and increase the hobby of eating fish. The location of service is carried out in Bulungala Village, Bonepantai District, Bone Bolano Regency, Gorontalo Province. The target of this service is the people of Bilungala Village, especially PKK women with activities carried out in November 2022. The activities consist of the preparation, implementation, and closing stages. In the preparatory stage, a survey was carried out in the village, then in the implementation stage, socialization was carried out to the community through the provision of material and ended with a discussion/question and answer regarding the material presented.
Calcium Assay of Tuna Bone Waste with Atomic Absorption Spectrophotometer (AAS) Najmah, Najmah; Ramadhani, Andi Indra Wulan Sari; Pagalla, Devi Bunga; Arviani, Arviani; Sangkota, Vivi Dia Afrianti
Hydrogen: Jurnal Kependidikan Kimia Vol 13, No 1 (2025): February 2025
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v13i1.14705

Abstract

Yellowfin tuna in Gorontalo is very abundant and is often exported outside the region and even abroad as fillets, so yellowfin tuna bones are also very plentiful. The community frequently processes yellowfin tuna bones into kuah asam or namely ilahe. One of the sources of ilahe is Averrhoa bilimbi L., but the leftover tuna bones are only a source of pollution. This research focuses on processing the waste into a product of high nutritional value and highlights the novelty of a more environmentally friendly fish bone calcium extraction method. One of the main novelties of this research is the use of natural materials, namely star fruit, as an alternative for calcium extraction, which reduces dependence on harmful chemicals and supports the principle of sustainability. The extraction method used heat, followed by drying to reduce moisture content, pulverizing (blender) to reduce particle size and sieving. The calcium content in tuna bone meal was then analyzed using the Atomic Absorption Spectroscopy (SSA) method. The results showed that the tuna bone meal was white in colour, and the calcium content was 184.4890 mg/g or 18.45%. This product has the potential to be applied in various fields, such as food, health, animal feed, pellets, and organic fertilizer, as well as a solution to reduce fishery waste and support environmental sustainability.
Co-Authors Abubakar Sidik Katili Adilah Nidaulhasanah Adju, Fitria Y.Hs. Ahaya, Cindi Ahmad Hidayat Alfiani, St Hijrah Ari Indrianto Arviani Arviani Arviani, Arviani Aulia Rahmadhani Chairunnisah J Lamangantjo D, Nurul Iqraini Endang Semiarti Evita Moni Damayanti Eyato, Rika febriyanti febriyanti Febriyanti, Febriyanti Fitria H.Ys. Adju Gubali, Hayati Ningsih Haerani Haerani Hartono D Mamu Hartono D. Mamu Hayatiningsih Gubali Herinda Mardin Indriati Husain Isima , Lindawaty Jusna Ahmad Kandowangko, Novri Youla Lamante, Nazwa S. Lilan Dama Magfirahtul Jannah Maryani Maryani Masra Latjompoh Masrid Pikoli Menanti, Menanti Miftahul F. Adudu Mustaqimah, Nur Najmah, Najmah Novri Youla Kandowangko Nur Mustaqimah Nurhayati Nurhayati Nurhayati Nurhayati Nursanti, Andi Nurul Fajryani Usman Putri, Restiyani Widia Ramadhani, Andi Indra Wulan Sari Ramlah Ramlah Ramlah Ramlah S. Ahmad, Sintia Sangkota, Vivi Dia Afrianti Tuada, Rasydah Nur Walangadi, Farhana Rahmatia