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All Journal Bioscientist : Jurnal Ilmiah Biologi
Suci Indah Putri
Universitas Andalas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Pengaruh Pemberian Nanopartikel TiO₂ pada Berbagai Konsentrasi terhadap Perubahan Morfologi dan Biomassa Kalus Ginseng Jawa (Talinum paniculatum) secara In Vitro Zakiy Maulana; Zozy Aneloi Noli; Suwirmen; Syifa Fajrisani; Suci Indah Putri
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18160

Abstract

This study aims to analyze the effect of titanium dioxide (TiO₂) nanoparticle concentrations on the characteristics of Talinum paniculatum (Java ginseng) callus. The experiment was conducted using a completely randomized design with six TiO₂ concentration treatments (0, 1, 2, 3, 4, and 5 ppm) over a 14-day incubation period. The observed parameters included callus morphology (color and texture), fresh weight, and dry weight. Fresh and dry weight data were analyzed using one-way analysis of variance (ANOVA) followed by Duncan’s New Multiple Range Test (DNMRT), while callus color and texture were analyzed descriptively. The results showed that the addition of TiO₂ nanoparticles induced changes in callus color ranging from yellow to yellow-reddish, and the resulting callus exhibited a compact texture. TiO₂ nanoparticles up to a concentration of 5 ppm did not show negative effects on either fresh or dry callus weight. These findings indicate that TiO₂ nanoparticles at the tested concentrations play a role in modifying the visual characteristics of callus without affecting biomass accumulation. Therefore, TiO₂ nanoparticles have potential application in enhancing secondary metabolite production.
Induction of Adventitious Roots from Leaf Explants of Talinum fruticosum (L.) Juss. through the Application of Different IBA Concentrations In Vitro Suci Indah Putri; Zozy Aneloi Noli; M. Idris
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 3 (2026): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i3.20669

Abstract

This study aimed to evaluate the effect of indole-3-butyric acid (IBA) concentrations on adventitious root induction from Talinum fruticosum leaf explants under in vitro conditions. The experiment was conducted using a completely randomized design with three IBA concentrations (2, 4, and 6 mg/L) and 20 replicates for each treatment. Leaf explants with an approximate diameter of 1.5 cm were cultured on Murashige and Skoog (MS) medium supplemented with IBA and observed for approximately 30 days. The evaluated parameters included explant survival percentage, root induction percentage, root initiation time, root number, root length, and root biomass (fresh and dry weight). Data were analyzed using analysis of variance (ANOVA). The results showed that IBA supplementation significantly affected root initiation time, with the fastest root emergence occurring after 8 days, and promoted root elongation, reaching a maximum root length of 1.23 cm. Explant survival and root induction percentages reached 100% in all treatments, indicating no significant differences among IBA concentrations. However, IBA application did not significantly affect root number or root biomass. Among the treatments, 2 mg/L IBA exhibited the best response by accelerating root initiation and increasing root length compared with the other concentrations. Therefore, the application of IBA at 2 mg/L effectively optimized selected parameters associated with adventitious root induction in T. fruticosum under in vitro conditions.
Co-Authors M. Idris Suwirmen, Suwirmen Syifa Fajrisani Zakiy Maulana Zozy Aneloi Noli