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All Journal Jurnal Ragam Pengabdian NAAFI: JURNAL ILMIAH MAHASISWA
Fitri Claudia Agathis Mahundingan
Universitas Pelita Harapan
Religion Economics, Econometrics & Finance Education Environmental Science Library & Information Science Social Sciences Other

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Analisis Metode Next-Generation Sequencing dalam Identifikasi Mutasi Genetik pada Streptoccocus pyogenes Terra Murti Balerura; Reva Laura Purba; Juneth Jennifer Sobalel; Fitri Claudia Agathis Mahundingan; Alifah Putri Safina
NAAFI: JURNAL ILMIAH MAHASISWA Vol. 2 No. 2 (2026): NAAFI: Jurnal Ilmiah Mahasiswa
Publisher : Pusat Penelitian dan Pengabdian (P3M) STKIP Majenang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62387/naafi.v2i2.415

Abstract

This study is a literature review aimed at analyzing the role of whole-genome sequencing (WGS) in the genomic characterization of Streptococcus pyogenes, particularly in relation to virulence, antimicrobial resistance, and mobile genetic elements. The study is motivated by the high incidence of infections and the limitations of conventional methods in providing comprehensive genetic resolution. Data collection was conducted through a systematic search of scientific articles in indexed databases, with relevant and up-to-date publications serving as research instruments. Data analysis employed a descriptive-comparative approach to evaluate sequencing quality, depth of genetic variation detection, and the ability to identify foreign genetic elements. The results indicate that WGS using a hybrid assembly approach combining Illumina and Oxford Nanopore technologies produces complete genome sequences with high accuracy (Q30; >99.9%) and 100% sequence identity. Furthermore, this method effectively detects antimicrobial resistance genes, including msr(D), mef(A), erm(B), and tet(M), and identifies prophage regions associated with increased virulence. The findings demonstrate that WGS provides higher analytical resolution than conventional methods and has strong potential to support molecular diagnostics and epidemiological surveillance. This study contributes to improving pathogen identification accuracy and enabling integrated antimicrobial resistance monitoring
Pengaruh Waktu Pengeringan Apusan Epitel Mukosa Oral terhadap Kualitas Pewarnaan Diff-Quik Suseno Adi Putra; Juneth Jennifer Sobalely; Fitri Claudia Agathis Mahundingan; Gabriella Aurel Kindangen; Diana Intan Gabriella Lusiana; Fransisca Probo Setyoningrum
Jurnal Ragam Pengabdian Vol. 3 No. 2 (2026): Mei-Agustus, Sustainable Development Goals (SDGs): Multidisciplinary Perspectiv
Publisher : Lembaga Teewan Journal Solutions

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62710/t5axea64

Abstract

Oral exfoliative cytology is a non-invasive diagnostic method widely used to evaluate morphological changes in oral mucosal epithelial cells. The quality of cytological preparations is influenced by several pre-analytical factors, including the air-drying time before Diff-Quik staining. This study aimed to determine the effect of different air-drying times on the staining quality of oral mucosal epithelial smears using the Diff-Quik method. A descriptive experimental design was employed with four air-drying time groups: 0, 5, 10, and 15 minutes. A total of 24 buccal smear preparations obtained from six healthy volunteers were evaluated. Slide quality was assessed based on nuclear clarity, cytoplasmic characteristics, and nuclear-cytoplasmic contrast using an objective scoring system. The results showed that all treatment groups achieved total scores ranging from 7 to 9, indicating good staining quality. The 0- and 5-minute air-drying groups produced the most optimal staining results, demonstrating clearer nuclear and cytoplasmic details, whereas the 10- and 15-minute groups exhibited a slight reduction in staining contrast in several preparations without significant morphological alterations. In conclusion, air-drying times of up to 15 minutes still produced cytological preparations with good staining quality, although 0–5 minutes provided the most optimal Diff-Quik staining results.
Co-Authors Alifah Putri Safina Diana Intan Gabriella Lusiana Gabriella Aurel Kindangen Juneth Jennifer Sobalel Juneth Jennifer Sobalely Reva Laura Purba Suseno Adi Putra Terra Murti Balerura