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TRAINING ON BIOCHEMICHAL IDENTIFICATION OF ENTEROBACTERIACEAE USING THE ENTEROSYSTEM 24R KIT TO STUDENTS OF MEDICAL LABORATORY TECHNOLOGY AT YARSI MEDIKA VOCATIONAL HIGH SCHOOL, TANGERANG Pangeran Andareas; Maroloan Aruan; Diana Intan Gabriella Lusiana; Maleakhi Samuel Mardiyono; Dolito Situmorang; Naomi Damayanti Purba; Nava Anabel Tahalele
Jurnal Sinergitas PKM & CSR Vol. 8 No. 3 (2024): June
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tri Dharma Perguruan Tinggi is the main foundation for universities in Indonesia in carrying out their functions and responsibilities. One of the activities that is part of the Tri Dharma of Higher Education is Community Service (CS). PkM activities have the aim of improving the welfare of the community by providing solutions to various problems that exist in the community. One solution that can be provided is to provide training to the community with the aim of increasing knowledge, skills or competencies of human resources (HR).  One of the important communities to improve competence is school students, especially vocational high schools (VHS) majoring in medical laboratory technology (MLT). This activity is important so that the graduates produced are in accordance with the needs of the world of work such as hospitals, clinics and research institutions. This activity was given to students majoring in medical laboratory technology, VHS Yarsi Medika Tangerang. The topic raised in this activity is training in the identification of enterobacteriaceae using the Enterosystem 24R kit. The data in this activity was obtained with a Quiz instrument (Pre-Test and Test) using Google Form.  The results obtained from PkM activities show that there is an increase in student knowledge related to PkM material by 35%.
Workshop Flebotomi: Peningkatan Keterampilan Pengambilan Darah Siswa Sekolah Dian Harapan Daan Mogot  Maroloan Aruan; Diana Intan Gabriella Lusiana; Tandry Meriyanti; Icha Paulina Simbolon; Maleakhi Samuel Mardiyono; Tiara Maasi; Ermi Maria Lukwaka
Jurnal Medika: Medika in progres
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/60zb7615

Abstract

Kegiatan Pengabdian kepada Masyarakat (PkM) ini dilatarbelakangi oleh kebutuhan siswa sekolah menengah untuk mengenal prosedur medis dasar sebelum memilih jurusan kuliah di bidang kesehatan. Kegiatan ini bertujuan memberikan pengetahuan dan keterampilan praktis teknik flebotomi (pengambilan darah vena dan kapiler) kepada siswa dan guru di SMA Sekolah Dian Harapan (SDH) Daan Mogot. Metode pelaksanaan kegiatan meliputi penyuluhan, demonstrasi, dan praktikum langsung (hands-on) menggunakan alat peraga manekin tangan flebotomi yang melibatkan 32 peserta. Evaluasi efektivitas pelatihan dilakukan melalui instrumen pre-test dan post-test. Hasil kegiatan menunjukkan peningkatan signifikan pada pemahaman peserta, dimana rata-rata nilai pengetahuan meningkat dari 65,97% pada pre-test menjadi 93,31% pada post-test. Analisis statistik menggunakan Paired Sample T-Test menunjukkan nilai signifikansi 0,003, dan perhitungan Normalized Gain Score (N-Gain) sebesar 0,71 yang masuk dalam kategori efektivitas tinggi. Peningkatan pemahaman tertinggi terlihat pada aspek teknis prosedural seperti sudut penusukan dan posisi bevel jarum. Disimpulkan bahwa workshop dengan metode simulasi manekin ini merupakan sarana yang efektif dan aman untuk menumbuhkan minat serta meningkatkan kompetensi dasar medis siswa tanpa risiko mencederai pasien nyata.
Pengaruh Pemberian Ekstrak Etil Asetat Buah Biwa (Eriobotrya japonica (Tunb.) Lindl) Terhadap Profil Mikrobiota Usus Tikus Yang Diinduksi Escherichia coli Diana Intan Gabriella Lusiana; Yesiska Kristina Hartanti; Pangeran Andareas; Justina Andrea Renjaan
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18372

Abstract

This study investigated the therapeutic potential of ethyl acetate extract derived from Biwa fruit on the gut microbiota profile of E. coli-infected mice. This study used 30 BALB-C mice divided into five groups: positive control (Ciprofloxacin and Lomperamide 130 mg/kgBW), negative control (CMC-Na), and three Biwa extract treatment groups at doses of 200, 400, and 600 mg/kgBW. The findings demonstrated that Biwa extract at 200 mg/kgBW exhibited significant efficacy from day one, resulting in complete suppression of E. coli growth in mice. However, the cohort receiving 400 mg/kgBW showed recurring E. coli growth on day two, potentially attributable to contamination. This research provides promising preliminary evidence of the potential of Biwa fruit as an alternative herbal-based prebiotic therapy to overcome E. coli infection.
Implementasi Manajemen Risiko Pengobatan Antihipertensi dengan Pendeketan Aplikasi TensiCare Berbasis Smartphone Febbyasi Megawaty Rangka; Jessica Novia; Diana Intan Gabriella Lusiana
CARADDE: Jurnal Pengabdian Kepada Masyarakat Vol. 8 No. 3 (2026): April
Publisher : Ilin Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31960/caradde.v8i3.3269

Abstract

Pengaruh Metode Pengambilan Sampel (Cotton Swab dan Cytobrush) Terhadap Kualitas Preparat Sitologi Oral Exfoliative Pada Pewarnaan Papanicolaou Putri Almellia; Ratna Anggun Kristiani Ndruru; Sara Agnes Julica Simanullang; Shaumi Anjani Ramadhani; Diana Intan Gabriella Lusiana; Fransisca Probo Setyoningrum
Jurnal Ragam Pengabdian Vol. 3 No. 2 (2026): Mei-Agustus, Sustainable Development Goals (SDGs): Multidisciplinary Perspectiv
Publisher : Lembaga Teewan Journal Solutions

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62710/gesa2e20

Abstract

Exfoliative oral cytology is a non-invasive screening method that supports the early detection of precancerous lesions and oral cavity cancers. The quality of the specimens produced for examination is influenced by preanalytical factors, including the type of sampling device used. This study aims to analyze the effect of using cotton swabs and cytobrushes on the quality of exfoliative oral cytology specimens stained with Papanicolaou using the Liquid-Based Cytology (LBC) method. This laboratory-based experimental study involved 18 healthy participants aged 18–25 years, selected using purposive sampling. Mucosal samples were collected using cotton swabs and cytobrushes, resulting in 36 preparations. All preparations were processed using the LBC method, stained with Papanicolaou stain, and then examined under a microscope to evaluate cell density, distribution, and the clarity of the preparation’s background. Differences in the surface characteristics between the cotton swab and the cytobrush were found to influence the quality of the resulting preparations. The cytobrush was deemed more effective for assessing cell density, whereas the cotton swab provided a cleaner background and better cell distribution. These findings can be considered when selecting sampling tools appropriate for the purpose of oral cytology examinations.  
Pengaruh Waktu Pengeringan Apusan Epitel Mukosa Oral terhadap Kualitas Pewarnaan Diff-Quik Suseno Adi Putra; Juneth Jennifer Sobalely; Fitri Claudia Agathis Mahundingan; Gabriella Aurel Kindangen; Diana Intan Gabriella Lusiana; Fransisca Probo Setyoningrum
Jurnal Ragam Pengabdian Vol. 3 No. 2 (2026): Mei-Agustus, Sustainable Development Goals (SDGs): Multidisciplinary Perspectiv
Publisher : Lembaga Teewan Journal Solutions

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62710/t5axea64

Abstract

Oral exfoliative cytology is a non-invasive diagnostic method widely used to evaluate morphological changes in oral mucosal epithelial cells. The quality of cytological preparations is influenced by several pre-analytical factors, including the air-drying time before Diff-Quik staining. This study aimed to determine the effect of different air-drying times on the staining quality of oral mucosal epithelial smears using the Diff-Quik method. A descriptive experimental design was employed with four air-drying time groups: 0, 5, 10, and 15 minutes. A total of 24 buccal smear preparations obtained from six healthy volunteers were evaluated. Slide quality was assessed based on nuclear clarity, cytoplasmic characteristics, and nuclear-cytoplasmic contrast using an objective scoring system. The results showed that all treatment groups achieved total scores ranging from 7 to 9, indicating good staining quality. The 0- and 5-minute air-drying groups produced the most optimal staining results, demonstrating clearer nuclear and cytoplasmic details, whereas the 10- and 15-minute groups exhibited a slight reduction in staining contrast in several preparations without significant morphological alterations. In conclusion, air-drying times of up to 15 minutes still produced cytological preparations with good staining quality, although 0–5 minutes provided the most optimal Diff-Quik staining results.