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All Journal JURNAL ANALIS LABORATORIUM MEDIK Science Midwifery
Setyoningrum, Fransisca Probo
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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PERBANDINGAN METODE DEHIDRASI TERHADAP KUALITAS PREPARAT PERMANEN LARVA NYAMUK Culex sp. Onesiforus, Benaya Yamin; Setyoningrum, Fransisca Probo; San, Maria Pritinsia Cici
Jurnal Analis Laboratorium Medik Vol 10 No 1 (2025): Jurnal Analis Laboratorium Medik
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v10i1.5527

Abstract

Proses pembuatan preparat larva nyamuk memiliki peran penting dalam dunia kesehatan untuk proses identifikasi. Salah satu tahap penitng dalam pembuatan preparat permanen larva nyamuk adalah tahap dehidrasi, yang dilakukan melalui perendaman larva kedalam alkohol. Banyaknya variasi pada prosedur perendaman etanol menjadikan belum adanya standar baku untuk proses dehidrasi dalam pembuatan preparat permanen larva nyamuk. Tujuan dari penelitian ini untuk mengetahui perbandingan 3 metode dehidrasi alkohol terhadap kualitas preparat permanen larva nyamuk. Penelitian ini merupakan penelitian eksperimental dengan menggunakan variasi dehidrasi etanol bertingkat. Jenis data yang diperoleh berupa data kategorik ordinal dan uji statistika yang digunakan dalam penelitian ini menggunakan uji Mann-Whitney U, pada kejernihan preparat didapatkan hasil, perlakuan alkohol 70% vs 30%,50%,96% nilai p = 0,609, alkohol 70% vs 60%,80%,90% nilai p = 0,159, antar alkohol bertingkat = 30%,50%,96% alkohol bertingkat 60%,80%,90% didapatkan nilai p = 0,357. Berdasarkan hasil penelitian yang telah dilakukan, dapat disimpulkan, tidak terdapat perbedaan yang signifikan antara metode perendaman alkohol terhadap kualitas preparat permanen lava nyamuk
SDS-PAGE characterization of dialyzed protein fractions from mycobacterium tuberculosis isolates for tuberculosis biomarker screening Barokah, Githa Agripina; Novitriani, Korry; Hasanah, Annisa Nur; Ferdiani, Dina; Setyoningrum, Fransisca Probo
Science Midwifery Vol 14 No 1 (2026): April: Health Sciences and related fields
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v14i1.2245

Abstract

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis, in which the identification of protein profiles serves as an important approach in diagnostic research and specific antigen development. This study aimed to analyze the protein profile of dialyzed fractions from Mycobacterium tuberculosis isolates using the SDS-PAGE (Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis) method. Protein isolation was carried out through sonication at 4 °C to lyse the cells and release intracellular proteins without causing denaturation. The resulting lysate supernatant was subjected to 30% ammonium sulfate precipitation to separate proteins based on their solubility, followed by dialysis to remove residual salts and unwanted small molecules. The total protein concentration was measured using a photometer with a total protein reagent, showing a relatively low value, indicating that the protein fraction was concentrated on specific molecules with higher purity. The SDS-PAGE analysis revealed the presence of a protein band with an estimated molecular weight of 77,6 kDa, suggesting the successful purification of protein fractions based on molecular weight. Based on these findings, it can be concluded that the combination of sonication, ammonium sulfate precipitation, and dialysis is effective as a preliminary purification method to obtain purer proteins from Mycobacterium tuberculosis isolates and enables more specific characterization of protein profiles through SDS-PAGE.
SDS-PAGE characterization of dialyzed protein fractions from mycobacterium tuberculosis isolates for tuberculosis biomarker screening Barokah, Githa Agripina; Novitriani, Korry; Hasanah, Annisa Nur; Ferdiani, Dina; Setyoningrum, Fransisca Probo
Science Midwifery Vol 14 No 1 (2026): April: Health Sciences and related fields
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v14i1.2333

Abstract

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis, in which the identification of protein profiles serves as an important approach in diagnostic research and specific antigen development. This study aimed to analyze the protein profile of dialyzed fractions from Mycobacterium tuberculosis isolates using the SDS-PAGE (Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis) method. Protein isolation was carried out through sonication at 4 °C to lyse the cells and release intracellular proteins without causing denaturation. The resulting lysate supernatant was subjected to 30% ammonium sulfate precipitation to separate proteins based on their solubility, followed by dialysis to remove residual salts and unwanted small molecules. The total protein concentration was measured using a photometer with a total protein reagent, showing a relatively low value, indicating that the protein fraction was concentrated on specific molecules with higher purity. The SDS-PAGE analysis revealed the presence of a protein band with an estimated molecular weight of 77,6 kDa, suggesting the successful purification of protein fractions based on molecular weight. Based on these findings, it can be concluded that the combination of sonication, ammonium sulfate precipitation, and dialysis is effective as a preliminary purification method to obtain purer proteins from Mycobacterium tuberculosis isolates and enables more specific characterization of protein profiles through SDS-PAGE.
Co-Authors Barokah, Githa Agripina Benaya Yamin Onesiforus Ferdiani, Dina Hasanah, Annisa Nur Korry Novitriani, Korry San, Maria Pritinsia Cici