Article Per Year (5 Year)
p-Index From 2021 - 2026
1.126
P-Index
This Author published in this journals
All Journal JURNAL ANALIS LABORATORIUM MEDIK Jurnal Ragam Pengabdian JURNAL MITRA MASYARAKAT (JMM)
Setyoningrum, Fransisca Probo
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 7 Documents
Search

 1 
PERBANDINGAN METODE DEHIDRASI TERHADAP KUALITAS PREPARAT PERMANEN LARVA NYAMUK Culex sp. Onesiforus, Benaya Yamin; Setyoningrum, Fransisca Probo; San, Maria Pritinsia Cici
Jurnal Analis Laboratorium Medik Vol 10 No 1 (2025): Jurnal Analis Laboratorium Medik
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v10i1.5527

Abstract

Proses pembuatan preparat larva nyamuk memiliki peran penting dalam dunia kesehatan untuk proses identifikasi. Salah satu tahap penitng dalam pembuatan preparat permanen larva nyamuk adalah tahap dehidrasi, yang dilakukan melalui perendaman larva kedalam alkohol. Banyaknya variasi pada prosedur perendaman etanol menjadikan belum adanya standar baku untuk proses dehidrasi dalam pembuatan preparat permanen larva nyamuk. Tujuan dari penelitian ini untuk mengetahui perbandingan 3 metode dehidrasi alkohol terhadap kualitas preparat permanen larva nyamuk. Penelitian ini merupakan penelitian eksperimental dengan menggunakan variasi dehidrasi etanol bertingkat. Jenis data yang diperoleh berupa data kategorik ordinal dan uji statistika yang digunakan dalam penelitian ini menggunakan uji Mann-Whitney U, pada kejernihan preparat didapatkan hasil, perlakuan alkohol 70% vs 30%,50%,96% nilai p = 0,609, alkohol 70% vs 60%,80%,90% nilai p = 0,159, antar alkohol bertingkat = 30%,50%,96% alkohol bertingkat 60%,80%,90% didapatkan nilai p = 0,357. Berdasarkan hasil penelitian yang telah dilakukan, dapat disimpulkan, tidak terdapat perbedaan yang signifikan antara metode perendaman alkohol terhadap kualitas preparat permanen lava nyamuk
Peningkatan Pengetahuan Pencegahan Soil-Transmitted Helminth (STH) melalui Edukasi Perilaku Hidup Bersih dan Sehat pada Anak di YAMAKINDO Agamonanza, Freddy; Setyoningrum, Fransisca Probo; Lusiana, Diana Intan Gabriella; Aruan, Maroloan; Tahya, Candra; Putri, Stella Migia; Sobalely, Juneth Jennifer
Jurnal Mitra Masyarakat (JMM) Vol. 7 No. 1 (2026): JURNAL MITRA MASYARAKAT
Publisher : STIKes Mitra Keluarga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47522/jmm.v7i1.246

Abstract

Soil-Transmitted Helminth (STH) merupakan infeksi cacing usus yang menjadi masalah kesehatan masyarakat, terutama pada anak sekolah di wilayah dengan sanitasi kurang memadai. Kegiatan Pengabdian kepada Masyarakat di Yayasan Mandiri Kreatif Indonesia (YAMAKINDO) bertujuan meningkatkan pengetahuan pencegahan STH melalui edukasi Perilaku Hidup Bersih dan Sehat (PHBS). Metode penelitian menggunakan pra-eksperimental dengan desain one group pretest-posttest. Intervensi dilaksanakan melalui ceramah interaktif, diskusi, praktik langsung langkah mencuci tangan, serta pemberian media edukasi dan obat cacing. Evaluasi menggunakan kuesioner terstruktur untuk mengukur tingkat pemahaman peserta. Hasil menunjukkan terdapat peningkatan pengetahuan mengenai jenis STH, penularan, dampak, dan pencegahan melalui PHBS. Meskipun skor pengetahuan meningkat, analisis statistik menunjukkan perbedaan yang tidak signifikan karena tingginya tingkat pengetahuan awal peserta. Akan tetapi peserta tetap antusias dan aktif. Kesimpulannya edukasi PHBS berkontribusi memperkuat pengetahuan pencegahan STH serta mendukung pembentukan perilaku sehat berkelanjutan.
Pengaruh Metode Pengambilan Sampel (Cotton Swab dan Cytobrush) Terhadap Kualitas Preparat Sitologi Oral Exfoliative Pada Pewarnaan Papanicolaou Almellia, Putri; Ndruru, Ratna Anggun Kristiani; Simanullang, Sara Agnes Julica; Ramadhani, Shaumi Anjani; Lusiana, Diana Intan Gabriella; Setyoningrum, Fransisca Probo
Jurnal Ragam Pengabdian Vol. 3 No. 2 (2026): Mei-Agustus, Sustainable Development Goals (SDGs): Multidisciplinary Perspectiv
Publisher : Lembaga Teewan Journal Solutions

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62710/gesa2e20

Abstract

Exfoliative oral cytology is a non-invasive screening method that supports the early detection of precancerous lesions and oral cavity cancers. The quality of the specimens produced for examination is influenced by preanalytical factors, including the type of sampling device used. This study aims to analyze the effect of using cotton swabs and cytobrushes on the quality of exfoliative oral cytology specimens stained with Papanicolaou using the Liquid-Based Cytology (LBC) method. This laboratory-based experimental study involved 18 healthy participants aged 18–25 years, selected using purposive sampling. Mucosal samples were collected using cotton swabs and cytobrushes, resulting in 36 preparations. All preparations were processed using the LBC method, stained with Papanicolaou stain, and then examined under a microscope to evaluate cell density, distribution, and the clarity of the preparation’s background. Differences in the surface characteristics between the cotton swab and the cytobrush were found to influence the quality of the resulting preparations. The cytobrush was deemed more effective for assessing cell density, whereas the cotton swab provided a cleaner background and better cell distribution. These findings can be considered when selecting sampling tools appropriate for the purpose of oral cytology examinations.  
Pengaruh Waktu Pengeringan Apusan Epitel Mukosa Oral terhadap Kualitas Pewarnaan Diff-Quik Putra, Suseno Adi; Sobalely, Juneth Jennifer; Mahundingan, Fitri Claudia Agathis; Kindangen, Gabriella Aurel; Lusiana, Diana Intan Gabriella; Setyoningrum, Fransisca Probo
Jurnal Ragam Pengabdian Vol. 3 No. 2 (2026): Mei-Agustus, Sustainable Development Goals (SDGs): Multidisciplinary Perspectiv
Publisher : Lembaga Teewan Journal Solutions

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62710/t5axea64

Abstract

Oral exfoliative cytology is a non-invasive diagnostic method widely used to evaluate morphological changes in oral mucosal epithelial cells. The quality of cytological preparations is influenced by several pre-analytical factors, including the air-drying time before Diff-Quik staining. This study aimed to determine the effect of different air-drying times on the staining quality of oral mucosal epithelial smears using the Diff-Quik method. A descriptive experimental design was employed with four air-drying time groups: 0, 5, 10, and 15 minutes. A total of 24 buccal smear preparations obtained from six healthy volunteers were evaluated. Slide quality was assessed based on nuclear clarity, cytoplasmic characteristics, and nuclear-cytoplasmic contrast using an objective scoring system. The results showed that all treatment groups achieved total scores ranging from 7 to 9, indicating good staining quality. The 0- and 5-minute air-drying groups produced the most optimal staining results, demonstrating clearer nuclear and cytoplasmic details, whereas the 10- and 15-minute groups exhibited a slight reduction in staining contrast in several preparations without significant morphological alterations. In conclusion, air-drying times of up to 15 minutes still produced cytological preparations with good staining quality, although 0–5 minutes provided the most optimal Diff-Quik staining results.
Perbandingan Efektivitas Cairan Fiksatif Ethanol 70% Komersial vs Neutral Buffered Formalin (NBF) 10% terhadap Morfologi Jaringan Hati dan Ginjal Putri, Agistha Naftalia; Parhusip, Daniel Larry Ferdyan Setiawan; Putri, Stella Migia; Gaol, Valentina Sumiharta Lumban; Lusiana, Diana Intan Gabriela; Setyoningrum, Fransisca Probo
Jurnal Ragam Pengabdian Vol. 3 No. 2 (2026): Mei-Agustus, Sustainable Development Goals (SDGs): Multidisciplinary Perspectiv
Publisher : Lembaga Teewan Journal Solutions

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62710/bed8qe75

Abstract

Fiksasi merupakan tahap penting dalam pengolahan jaringan histologi karena berperan dalam mempertahankan morfologi jaringan agar tetap mendekati kondisi aslinya. Neutral Buffered Formalin 10% merupakan fiksatif yang umum digunakan dalam pemeriksaan histopatologi, sedangkan ethanol 70% sering dimanfaatkan sebagai alternatif karena mudah diperoleh dan relatif lebih aman. Penelitian ini bertujuan untuk membandingkan efektivitas ethanol 70% komersial dan Neutral Buffered Formalin 10% terhadap morfologi jaringan hati dan ginjal mencit. Penelitian menggunakan metode eksperimental laboratorium dengan sampel jaringan hati dan ginjal yang difiksasi menggunakan kedua jenis fiksatif, kemudian diproses secara histologi rutin dan diwarnai menggunakan Hematoksilin-Eosin. Penilaian morfologi dilakukan berdasarkan kualitas inti sel, sitoplasma, dan keseragaman warna. Hasil penelitian menunjukkan bahwa pada jaringan hati terdapat perbedaan bermakna pada parameter inti sel dan keseragaman warna, sedangkan pada jaringan ginjal terdapat perbedaan bermakna pada parameter sitoplasma dan keseragaman warna. Secara keseluruhan, preparat yang difiksasi menggunakan Neutral Buffered Formalin 10% menunjukkan kualitas morfologi yang lebih baik dibandingkan ethanol 70% komersial. Penelitian ini menyimpulkan bahwa Neutral Buffered Formalin 10% lebih efektif dalam mempertahankan morfologi jaringan hati dan ginjal mencit pada preparat histologi rutin
Komparasi Efektivitas Minyak Pinus dan Minyak Alpukat Sebagai Pengganti Xylene Dalam Proses Penjernihan (Clearing) Histologi Kawer, Joy Miracle Tumigolung; Cheriananda , Yessica Stefanie; Tumansery, Margareth; Kamengon, Alessandro Stefano; Lusiana, Diana Intan Gabriella; Setyoningrum, Fransisca Probo
Jurnal Ragam Pengabdian Vol. 3 No. 2 (2026): Mei-Agustus, Sustainable Development Goals (SDGs): Multidisciplinary Perspectiv
Publisher : Lembaga Teewan Journal Solutions

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62710/78vbt377

Abstract

Xylene is a standard clearing agent in histological tissue processing; however, its use poses a toxicity risk to the health of laboratory staff, making it necessary to find safer natural alternatives, such as pine oil and avocado oil. This experimental laboratory study aimed to compare the effectiveness of pine oil and avocado oil as xylene substitutes in improving the quality of mouse liver histological preparations. A total of twenty-seven mouse liver tissue sections were divided into three treatment groups: a xylene control group, an avocado oil group, and a pine oil group. The sections were then stained using the hematoxylin-eosin method and evaluated using a scoring system that assessed nuclear chromatin integrity, cytoplasmic contrast, preservation of tissue architecture, and microtome section quality. The results showed that pine oil achieved the highest total score, with mechanical performance comparable to xylene and superior preservation of cellular structure, while avocado oil demonstrated good cytoplasmic contrast and nuclear integrity but had limitations in section quality due to its high viscosity. Xylene itself excelled in ease of sectioning but resulted in poor nuclear integrity and cytoplasmic contrast due to repeated use. This study concludes that pine oil is the most effective and environmentally friendly alternative clarifying agent, while avocado oil still requires further process optimization before it can be widely recommended.
Efektivitas Giemsa sebagai Pengganti Pewarna Papanicolaou pada Sitologi Urin Salim, Jesslyn Widya; Aditya, Christian Dame; Purba, Christine Vania; Sau, Kasamira Sukacita Uliarti; Lusiana, Diana Intan Gabriella; Setyoningrum, Fransisca Probo
Jurnal Ragam Pengabdian Vol. 3 No. 2 (2026): Mei-Agustus, Sustainable Development Goals (SDGs): Multidisciplinary Perspectiv
Publisher : Lembaga Teewan Journal Solutions

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62710/aqrn9h21

Abstract

Papanicolaou staining is a common or most frequently used staining method in cytology because it provides a highly detailed view of cell nuclei and cytoplasmic transparency. Giemsa staining is generally used on blood smears, but it can also provide a highly detailed view of cells, including epithelial cells. This study aims to investigate and evaluate the effectiveness of Giemsa staining as a substitute for Papanicolaou staining in urinary cytology. The research method employed was a descriptive experimental laboratory study evaluating twenty urine cytology slides stained with a 25% Giemsa solution, using Papanicolaou staining as the reference method. The slides were evaluated based on cell morphology, nuclear contrast, cytoplasmic contrast, and background clarity. Most of the urine cytology slides stained with Giemsa were classified as “Good” (50%) or “Very Good” (35%), with no slides classified as “Not Good.” Giemsa staining preserves nuclear morphology but shows lower cytoplasmic transparency compared to Papanicolaou staining. 
Co-Authors Aditya, Christian Dame Agamonanza, Freddy Almellia, Putri Aruan, Maroloan Benaya Yamin Onesiforus Cheriananda , Yessica Stefanie Gaol, Valentina Sumiharta Lumban Kamengon, Alessandro Stefano Kawer, Joy Miracle Tumigolung Kindangen, Gabriella Aurel Lusiana, Diana Intan Gabriela Lusiana, Diana Intan Gabriella Mahundingan, Fitri Claudia Agathis Ndruru, Ratna Anggun Kristiani Parhusip, Daniel Larry Ferdyan Setiawan Purba, Christine Vania Putra, Suseno Adi Putri, Agistha Naftalia Putri, Stella Migia Ramadhani, Shaumi Anjani Salim, Jesslyn Widya San, Maria Pritinsia Cici Sau, Kasamira Sukacita Uliarti Simanullang, Sara Agnes Julica Sobalely, Juneth Jennifer Tahya, Candra Tumansery, Margareth