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All Journal Medical Journal of Indonesia Acta Biochimica Indonesiana
Gantini, Ria Syafitri Evi
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Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Maternal IgG in hemolytic disease of the fetus and newborn-ABO incompatibility Wibowo, Heri; Nurrahmah, Sheila; Gantini, Ria Syafitri Evi
Medical Journal of Indonesia Vol. 33 No. 2 (2024): June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.247269

Abstract

BACKGROUND Hemolytic disease of the fetus and newborn (HDFN) is a type of anemia in the fetus or newborn, characterized by anemia, jaundice, hyperbilirubinemia, and brain damage. IgG is the only antibody that can cross the placenta. The IgG subtypes have a different ability to destroy red blood cells (RBCs). IgG1 and IgG3 can bind to Fc-phagocyte cell receptors and cause hemolysis, while IgG3 has more ability than IgG1. This study aimed to identify the antibody IgG subtype contributing to clinical manifestation differences in HDFN. METHODS This study used blood and umbilical cord blood samples from 30 pairs of mother-baby. The samples were grouped into control (not jaundice/normal bilirubin levels) and jaundice/hyperbilirubinemia groups. A self-developed IgG subtype enzyme-linked immunosorbent assay protocol was performed on maternal samples, resulting in optical density. Statistical analysis was performed using SPSS software version 23. RESULTS Blood type was associated with total bilirubin expression (p = 0.005). IgG1 anti-A, IgG3 anti-A, IgG4 anti-A, IgG1 anti-B, IgG3 anti-B, and IgG4 anti-B significantly affected hyperbilirubinemia in newborns (p = 0.041, 0.013, 0.017, 0.028, 0.001, and 0.007, respectively). CONCLUSIONS IgG1 and IgG3 were more significant in causing clinical problems. IgG4 suppressed IgG activation, resulting in no destruction of the infant’s RBCs.
Purification of total IgG from sars-cov-2 convalescent serum Lusinanto, Arfat; Gantini, Ria Syafitri Evi; Gunarti, Dwirini Retno; Sadikin, Mohamad
Acta Biochimica Indonesiana Vol. 8 No. 2 (2025): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.203

Abstract

Background: Convalescent plasma contains neutralizing antibodies against SARS-CoV-2 but also potentially harmful inflammatory cytokines. Purified immunoglobulin G fractions offer a safer therapeutic alternative by concentrating antibodies while removing inflammatory mediators. Objective: To establish a systematic protocol for purifying total IgG from SARS-CoV-2 convalescent serum using sequential chromatographic techniques. Methods: Serum from 90 recovered donors was pooled into three independent samples. Purification employed four sequential steps: ammonium sulfate precipitation (50% saturation), Sephadex G-100 size-exclusion chromatography, DEAE-Cellulose ion-exchange chromatography, and Protein A affinity chromatography. Purity was assessed by native polyacrylamide gel electrophoresis and radial immunodiffusion. Results: Starting from serum with 19.68 ± 7.27 mg/mL IgG and 110.47 ± 11.99 mg/mL total protein, the four-step purification yielded final IgG concentration of 1.14 ± 0.70 mg/mL with 1.19 ± 0.16 mg/mL total protein. This achieved 6.3-fold purification with purity ratio of 1.01 ± 0.38 and 5.8% recovery. Native PAGE confirmed high purity with a single IgG band. Conclusion: Sequential chromatography successfully purified total IgG from convalescent serum, providing a laboratory-scale method for preparing safer immunoglobulin therapeutics.
Co-Authors Dwirini Retno Gunarti Heri Wibowo Lusinanto, Arfat Mohamad Sadikin Nurrahmah, Sheila