ANNISA ANNISA
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran. Jl. Raya Bandung-Sumedang Km 21, Jatinangor, Sumedang 45363, West Java, Indonesia

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

Characterization and identification of three thermophilic Bacillus strain isolated from Domas Crater, Mt. Tangkuban Perahu, Indonesia RATU SAFITRI; DELICIA PUTRI KUSUMAWARDHANI; ANNISA ANNISA; Ruhyat Partasasmita; SINTA ASHARINA; ANI MELANI MASKOEN
Biodiversitas Journal of Biological Diversity Vol. 21 No. 8 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210805

Abstract

Abstract. Safitri R, Kusumawardhani PD, Annisa, Partasasmita R, Asharina S, Maskoen AM. 2020. Characterization and identification of three thermophilic Bacillus strains isolated from Domas Crater, Mt. Tangkuban Perahu, Indonesia. Biodiversitas 21: 3444-3453. A community of thermophiles within the hot spring of Domas Crater, Mount Tangkuban West Java, has been cultivated and identified based on the 16S ribosomal RNA gene sequence. The hot spring has a temperature of 45°C-76 °C, pH 1-2, the isolate has been cultivated in Thermus medium at a temperature of 70 and pH 6. The three isolated strains were L61A, TS61A, and D41A identified based on the 16S ribosomal RNA gene sequence refer to the GenBank database and all of them belong to the Phylum Firmicutes, which are clustered within the taxonomic groups of Bacillus, the Bacillus, and Geobacillus genera. Nucleotide sequences were compared for homology with BLASTN search at the NCBI Web-site revealed that strains (L61A) had 99.93% similarity with Geobacillus uzenensis. TS61A strain has a 99.24% similarity with Bacillus wiedmannii. Whereas D41A has 99.79% similarity with Bacillus paramycoides. The characterization also includes the phenotypic and biochemical of strains. The bacillus genera were known as a source of thermostable enzymes, plant growth-promoting, probiotics, decomposers of various polysaccharide materials, and bioremediation.
Ethnobotany of cassava (Manihot esculenta Crantz) in the traditional peri-urban Sundanese community of Cireundeu Hamlet, Cimahi City, West Java, Indonesia BUDI IRAWAN; KARINA MAHESWARI SAFIRA; ANNISA ANNISA; ENENG NUNUZ ROHMATULLAYALY; MUHAMMAD IHSAN; SURYANA SURYANA; JOHAN ISKANDAR; BUDIAWATI S. ISKANDAR
Biodiversitas Journal of Biological Diversity Vol. 25 No. 1 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250113

Abstract

Abstract. Irawan B, Safira KM, Annisa, Rohmatullayaly EN, Ihsan M, Suryana, Iskandar J, Iskandar BS. 2024. Ethnobotany of cassava (Manihot esculenta) in the traditional peri-urban Sundanese community of Cireundeu Hamlet, Cimahi City, West Java, Indonesia. Biodiversitas 25: 116-128. The community of Cireundeu Hamlet is one of the Sundanese traditional communities that still maintain wisdom in processing and using cassava (Manihot esculenta Crantz) as staple food (rasi) and foodstuff. Many landraces of cassava have the potential to develop into superior varieties, and research is necessary to make cassava a year-round food. The aim of this study is to elucidate the ethnobotanical knowledge of the rural Sundanese community on cassava, namely on various landraces and their folk classification, traditional farming management, and uses. Based on a case study of the traditional people in a peri-urban area of Cireundeu Hamlet, Cimahi City, West Java, Indonesia, mixed methods, i.e., qualitative and quantitative, were used in this study. There are seven landraces of cassava used as foodstuff that are traditionally managed by the Cireundeu people, including sampeu apuy, sampeu bodas, sampeu gambir lembang, sampeu garnawis, sampeu hideung, sampeu karihkil, and sampeu mentega. Based on Relative Frequency of Citation, the highest values are sampeu bodas (0.96) and sampeu garnawis (0.93). These cassava landraces can be distinguished by the Cireundeu people based on characteristics of petiole color, stem color, apical leaf color, leaf color, and taste of the tubers. Based on folk taxonomy, cassava is classified into five levels: level 0 (unique beginner), known as tutuwuhan and pepelakan (plants); level 1 (Life form) known as beubeutian (tubers); level 2 (generic) known as sampeu karet and sampeu, a genus from Manihot esculenta; level 3 (species) known as sampeu (sweet-cassava, Manihot esculenta Crantz) and sampeu karet (bitter cassava, M. carthagenensis subsp. glaziovii); and level 4 (specific). The processing and use of cassava are carried out both in traditional and modern ways, including using apical leaves as lalab or boiled vegetables and using the roots as the basic ingredients for making rasi (sangueun), tapioca flour, and snacks.