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All Journal Biodiversitas Journal of Biological Diversity
Irawati Wahyu
Department of Biology Education, Faculty of Education, Universitas Pelita Harapan. Jl. M.H. Thamrin Boulevard 1100, Lippo Karawaci, Tangerang 15811, Banten, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Indigenous copper resistant bacteria isolated from activated sludge of water treatment plant in Surabaya, Indonesia Irawati Wahyu; REINHARD PINONTOAN; TRIWIBOWO YUWONO
Biodiversitas Journal of Biological Diversity Vol. 21 No. 11 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d211112

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Abstract. Irawati W, Pinontoan R, Yuwono T. 2020. Indigenous copper resistant bacteria isolated from activated sludge of water treatment plant in Surabaya, Indonesia. Biodiversitas 21: 5077-5084. Biological wastewater treatment using activated sludge is a promising wastewater treatment solution for removing heavy metals. To improve the effectiveness of biological wastewater treatment, activated sludge must consist of bacteria that can remove heavy metals through the process of bioaccumulation and biosorption. This study was aimed to isolate indigenous copper resistant bacteria and determining their resistance to copper, as well as analyzing their ability to accumulate and remove copper. Copper resistant bacteria were isolated from activated sludge of water treatment plant in industrial plant in Rungkut, Surabaya. Resistance to copper was analyzed by determining the value of minimum inhibitory concentration (MIC). The ability of bacterial isolates to remove copper was analyzed by atomic absorption spectrophotometer. A total of six highly copper resistant bacteria were isolated and designated as B6.1, C8.1, C9.3, C9.4, C9.5, C10.4 isolates. All isolates were categorized as high resistant bacteria with the MICs of 9-11 mM CuSO4. The two highest copper resistant bacteria were isolates C10.4 and C9.4. The ability of the two isolates to accumulate copper was 8.02 mg and 4.83 mg per gram dry weight of cells and to remove of copper up to 20.45% and 17.66%, respectively.
Indigenous multiresistant bacteria of Cupriavidus pauculus IrC4 isolated from Indonesia as a heavy metal bioremediation agent Irawati Wahyu; STEVANUS ERICK WINOTO; LUCIA KUSUMAWATI; REINHARD PINONTOAN
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220641

Abstract

Abstract. Irawati W, Winoto SE, Kusumawati L, Pinontoan R. 2021. Indigenous multiresistant bacteria of Cupriavidus pauculus IrC4 isolated from Indonesia as a heavy metal bioremediation agent. Biodiversitas 22: 3349-3355. Heavy metal pollution is a serious environmental problem because it endangers humans, animals, and plants. Bioremediation of heavy metals using bacteria is an effective method to remove heavy metals. Cupriavidus pauculus IrC4 is an indigenous multi-resistant bacteria isolated from Indonesia. This study aims to determine the growth of this strain in a medium containing cadmium, mercury, lead, copper, and its ability to accumulate heavy metal. Bacterial resistance was observed by cultivating bacteria on a Luria Bertani medium containing various concentrations of heavy metals. Heavy metal accumulation was measured using atomic absorption spectrophotometer. The study showed that this strain could grow in a solid medium containing 5 mM cadmium, 13 mM lead, and 4 mM mercury, also in 0.5 mM of the heavy metal mixture. A high concentration of heavy metals resulted in lag phase elongation and logarithmic growth phase inhibition. C. pauculus IrC4 could accumulate copper, lead, and cadmium and lead up to 371.42 mg, 254.4 mg, 5.8 mg heavy metals per gram of dry weight of cells, respectively. In conclusion, this strain is a promising bacterium for use as a heavy metal bioremediation agent.
Co-Authors LUCIA KUSUMAWATI REINHARD PINONTOAN STEVANUS ERICK WINOTO TRIWIBOWO YUWONO