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All Journal Biodiversitas Journal of Biological Diversity
REINHARD PINONTOAN
Department of Biology, Faculty of Science and Technology, Universitas Pelita Harapan. Jl. M.H. Thamrin Boulevard 1100, Lippo Karawaci, Tangerang 15811, Banten, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Indigenous copper resistant bacteria isolated from activated sludge of water treatment plant in Surabaya, Indonesia Irawati Wahyu; REINHARD PINONTOAN; TRIWIBOWO YUWONO
Biodiversitas Journal of Biological Diversity Vol. 21 No. 11 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d211112

Abstract

Abstract. Irawati W, Pinontoan R, Yuwono T. 2020. Indigenous copper resistant bacteria isolated from activated sludge of water treatment plant in Surabaya, Indonesia. Biodiversitas 21: 5077-5084. Biological wastewater treatment using activated sludge is a promising wastewater treatment solution for removing heavy metals. To improve the effectiveness of biological wastewater treatment, activated sludge must consist of bacteria that can remove heavy metals through the process of bioaccumulation and biosorption. This study was aimed to isolate indigenous copper resistant bacteria and determining their resistance to copper, as well as analyzing their ability to accumulate and remove copper. Copper resistant bacteria were isolated from activated sludge of water treatment plant in industrial plant in Rungkut, Surabaya. Resistance to copper was analyzed by determining the value of minimum inhibitory concentration (MIC). The ability of bacterial isolates to remove copper was analyzed by atomic absorption spectrophotometer. A total of six highly copper resistant bacteria were isolated and designated as B6.1, C8.1, C9.3, C9.4, C9.5, C10.4 isolates. All isolates were categorized as high resistant bacteria with the MICs of 9-11 mM CuSO4. The two highest copper resistant bacteria were isolates C10.4 and C9.4. The ability of the two isolates to accumulate copper was 8.02 mg and 4.83 mg per gram dry weight of cells and to remove of copper up to 20.45% and 17.66%, respectively.
Indigenous multiresistant bacteria of Cupriavidus pauculus IrC4 isolated from Indonesia as a heavy metal bioremediation agent Irawati Wahyu; STEVANUS ERICK WINOTO; LUCIA KUSUMAWATI; REINHARD PINONTOAN
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220641

Abstract

Abstract. Irawati W, Winoto SE, Kusumawati L, Pinontoan R. 2021. Indigenous multiresistant bacteria of Cupriavidus pauculus IrC4 isolated from Indonesia as a heavy metal bioremediation agent. Biodiversitas 22: 3349-3355. Heavy metal pollution is a serious environmental problem because it endangers humans, animals, and plants. Bioremediation of heavy metals using bacteria is an effective method to remove heavy metals. Cupriavidus pauculus IrC4 is an indigenous multi-resistant bacteria isolated from Indonesia. This study aims to determine the growth of this strain in a medium containing cadmium, mercury, lead, copper, and its ability to accumulate heavy metal. Bacterial resistance was observed by cultivating bacteria on a Luria Bertani medium containing various concentrations of heavy metals. Heavy metal accumulation was measured using atomic absorption spectrophotometer. The study showed that this strain could grow in a solid medium containing 5 mM cadmium, 13 mM lead, and 4 mM mercury, also in 0.5 mM of the heavy metal mixture. A high concentration of heavy metals resulted in lag phase elongation and logarithmic growth phase inhibition. C. pauculus IrC4 could accumulate copper, lead, and cadmium and lead up to 371.42 mg, 254.4 mg, 5.8 mg heavy metals per gram of dry weight of cells, respectively. In conclusion, this strain is a promising bacterium for use as a heavy metal bioremediation agent.
Exploration of indigenous copper and dye-resistant bacteria isolated from Citarum River, West Java, Indonesia WAHYU IRAWATI; DWI NINGSIH SUSILOWATI; INDAH SOFIANA; VALENTINE LINDARTO; REINHARD PINONTOAN; TRIWIBOWO YUWONO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 2 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240263

Abstract

Abstract. Irawati W, Susilowati DN, Sofiana I, Lindarto V, Pinontoan R, Yuwono T. 2023. Exploration of indigenous copper and dye-resistant bacteria isolated from Citarum River, West Java, Indonesia. Biodiversitas 24: 1215-1223. Bacterial bioremediation utilizing indigenous bacteria has been reported as an effective, economical, and eco-friendly solution to marine contamination. However, study on the use of dye and copper-resistant bacteria has not been done much. The study aimed to isolate copper and dye-resistant bacteria, determining copper and dye resistance and decolorization abilities. Copper and dye-resistant bacteria were isolated from the Citarum River, West Java, Indonesia. Bacterial isolates were identified based on 16S rDNA gene analysis. Copper resistance was determined by measuring the minimum inhibitory concentration (MIC) of CuSO4. Dye resistance was observed by growing the bacterial isolates on a medium containing 100-500 ppm of various dyes. The dye decolorization was analyzed by monitoring the absorbance of each dye using a spectrophotometer. Fifty-four of copper indigenous resistant bacteria have been isolated. Nine bacterial isolates that showed high resistance to copper and dye with the MIC of 11 mM CuSO4 were identified as Siccibacter colletis, Acinetobacter baumannii, Lysinibacillus fusiformis, Bacillus cereus, and Escherichia coli. The highest multi-resistant bacterium was Bacillus cereus CTR 200 3.2 with decolorization rates of 93.04%, 61.9%, and 87.43% on 100 ppm methylene blue, malachite green, and basic fuchsine dye, respectively. However, adding 5 mM CuSO4 reduced those decolorization rates to 39.39%, 10.48%, and 7.39%, respectively.
The fibrinolytic potential of Bacillus amyloliquefaciens isolates from salt-fermented shrimp paste terasi REINHARD PINONTOAN; SANNIA CITY; ANASTHASIA NATHANIA WIDJAJA; JONATHAN SUCIONO PURNOMO; DIKSON DIKSON
Biodiversitas Journal of Biological Diversity Vol. 25 No. 7 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250740

Abstract

Abstract. Pinontoan R, City S, Widjaja AN, Purnomo JS, Dikson. 2024. The fibrinolytic potential of Bacillus amyloliquefaciens isolates from salt-fermented shrimp paste terasi. Biodiversitas 25: 3193-3199. Thrombosis, a major pathology in Cardiovascular Diseases (CVDs), significantly contributes to global mortality. Although medicinal prevention and management of recurrent thromboses do exist, alternative means using natural sources are actively sought because of their lower costs, better compatibility, and lower risks of side effects. Due to their beneficial microorganisms, fermented foods offer a potential thrombolytic source for managing CVDs. In this study, we aimed to isolate and identify bacteria with thrombolytic activity from fermented shrimp paste terasi. Potential protease-producing bacteria from terasi were determined via cell and colony morphology, biochemical properties, and 16S rRNA sequence analyses. Subsequently, the thrombolytic and fibrinolytic activities of the bacteria were assessed by performing whole-blood clot tests and fibrin degradation assays; two protease-producing bacteria, designated as TJU5 and TMAD4 isolates were identified as Bacillus amyloliquefaciens. The isolates demonstrated thrombolytic activity by significantly reducing whole-blood clot mass after 2 h of incubation. The thrombolytic mechanism involves fibrinolysis indicated by the rapid degradation of A?, B?, and ? fibrin chains observed within 1 min of incubation. These findings highlight the beneficial bacteria from fermented shrimp paste terasi, identified as B. amyloliquefaciens TJU5 and TMAD4, with high thrombolytic and fibrinolytic activities, underscoring their potential role in bolstering cardiovascular health.
Co-Authors ANASTHASIA NATHANIA WIDJAJA DIKSON DIKSON DWI NINGSIH SUSILOWATI INDAH SOFIANA Irawati Wahyu JONATHAN SUCIONO PURNOMO LUCIA KUSUMAWATI SANNIA CITY STEVANUS ERICK WINOTO TRIWIBOWO YUWONO VALENTINE LINDARTO WAHYU IRAWATI