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TRIWIBOWO YUWONO
Department of Agricultural Microbiology, Faculty of Agriculture, Universitas Gadjah Mada. Jl. Flora No. 1, Bulaksumur, Sleman 55281, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Indigenous copper resistant bacteria isolated from activated sludge of water treatment plant in Surabaya, Indonesia Irawati Wahyu; REINHARD PINONTOAN; TRIWIBOWO YUWONO
Biodiversitas Journal of Biological Diversity Vol. 21 No. 11 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d211112

Abstract

Abstract. Irawati W, Pinontoan R, Yuwono T. 2020. Indigenous copper resistant bacteria isolated from activated sludge of water treatment plant in Surabaya, Indonesia. Biodiversitas 21: 5077-5084. Biological wastewater treatment using activated sludge is a promising wastewater treatment solution for removing heavy metals. To improve the effectiveness of biological wastewater treatment, activated sludge must consist of bacteria that can remove heavy metals through the process of bioaccumulation and biosorption. This study was aimed to isolate indigenous copper resistant bacteria and determining their resistance to copper, as well as analyzing their ability to accumulate and remove copper. Copper resistant bacteria were isolated from activated sludge of water treatment plant in industrial plant in Rungkut, Surabaya. Resistance to copper was analyzed by determining the value of minimum inhibitory concentration (MIC). The ability of bacterial isolates to remove copper was analyzed by atomic absorption spectrophotometer. A total of six highly copper resistant bacteria were isolated and designated as B6.1, C8.1, C9.3, C9.4, C9.5, C10.4 isolates. All isolates were categorized as high resistant bacteria with the MICs of 9-11 mM CuSO4. The two highest copper resistant bacteria were isolates C10.4 and C9.4. The ability of the two isolates to accumulate copper was 8.02 mg and 4.83 mg per gram dry weight of cells and to remove of copper up to 20.45% and 17.66%, respectively.
Burkholderia cepacia strain IrV1 multi-resistant to copper and dyes isolated from laboratory wastewater effluent WAHYU IRAWATI; VALENTINE LINDARTO; REINHARD PINONTOAN; TRIWIBOWO YUWONO; FEBRI MANGOMO MANGUNSONG; DESTYA WATY SILALAHI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 5 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230544

Abstract

Abstract. Irawati W, Lindarto V, Pinontoan R, Yuwono T, Mangunsong FM, Silalahi DW. 2022. Burkholderia cepacia strain IrV1 multi-resistant to copper and dyes isolated from laboratory wastewater effluent. Biodiversitas 23: 2614-2620. Copper and dye multi-resistant bacteria may be cultivated to increase the efficiency of waste bioremediation processes. Multi-resistant bacteria can be isolated from waste containing copper and dyes, including laboratory waste. The aims of this study were to: (i) isolate copper-resistant bacteria from wastewater, (ii) determine the multi-resistance of bacteria against copper and dyes, and (iii) observe the ability of bacteria to remove colors methylene blue, basic fuchsin, and Congo red. Selected bacterial isolates were identified based on the 16S rDNA gene. One highly copper resistant bacterial strain IrV, showed resistance to 7 mM CuSO4, 100 ppm methylene blue, 100 ppm basic fuchsin, and 100 ppm Congo red. Based on the 16S rDNA gene sequence, strain IrV belongs to Burkholderia cepacia with 99.86% similarity. The addition of copper on the medium resulted in colony color changes from light yellow to green indicated Cu binding within the cell. Moreover, a clear zone around the colony was observed, suggesting that the strain is capable of decolorizing methylene blue, Congo red, and basic fuchsin dyes. The results thus demonstrate that bacterial strain IrV is multi-resistant to copper and dyes.
Enterobacter hormaechei KIMS8 and Enterobacter cloacae KIMS10 isolated from Kapuas River, Kalimantan, Indonesia as indigenous multi-resistant bacteria to copper and dyes WAHYU IRAWATI; MICHAEL TIMOTHY; SAMUEL EMMANUEL SOENTORO; REINHARD PINONTOAN; TRIWIBOWO YUWONO; VALENTINE LINDARTO
Biodiversitas Journal of Biological Diversity Vol. 23 No. 12 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231265

Abstract

Abstract. Irawati W, Timothy M, Soentoro SE, Pinontoan R, Yuwono T, Lindarto V. 2022. Enterobacter hormaechei KIMS8 and Enterobacter cloacae KIMS10 isolated from Kapuas River, Kalimantan, Indonesia as indigenous multi-resistant bacteria to copper and dyes. Biodiversitas 23: 6661-6668. Study on the characterization of multiple copper and dyes resistance in bacteria has so far been quite limited. It is, therefore, of interest to isolate and characterize such bacteria. The study was aimed to isolate bacteria from the Kapuas River in Indonesia and measuring copper resistance as well as dye-resistance and decolorization abilities. Copper resistance was determined by measuring the minimum inhibitory concentration (MIC) of copper, while dye-resistance was measured by observing changes in colony color and clear zone formation and decolorization ability was determined by spectrophotometry. It was found that 2 out of the 15 isolated strains showed the highest copper resistance with the MIC of 7 mM. The two bacterial strains KIMS8 and KIMS10, were grown in solid media supplemented with 300 ppm of methylene blue or reactive black dye. Bacterial decolorization assays showed that KIMS8 was able to decolorize up to 90% methylene dye and up to 11.3% of reactive black dye whereas KIMS10 was able to decolorize 94.9% of methylene blue dye and 12.1% of reactive black dye. Molecular characterization by 16S rRNA gene sequencing of these two strains showed that KIMS8 and KIMS10 were identified as Enterobacter hormaechei and E. cloacae, respectively.
Exploration of indigenous copper and dye-resistant bacteria isolated from Citarum River, West Java, Indonesia WAHYU IRAWATI; DWI NINGSIH SUSILOWATI; INDAH SOFIANA; VALENTINE LINDARTO; REINHARD PINONTOAN; TRIWIBOWO YUWONO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 2 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240263

Abstract

Abstract. Irawati W, Susilowati DN, Sofiana I, Lindarto V, Pinontoan R, Yuwono T. 2023. Exploration of indigenous copper and dye-resistant bacteria isolated from Citarum River, West Java, Indonesia. Biodiversitas 24: 1215-1223. Bacterial bioremediation utilizing indigenous bacteria has been reported as an effective, economical, and eco-friendly solution to marine contamination. However, study on the use of dye and copper-resistant bacteria has not been done much. The study aimed to isolate copper and dye-resistant bacteria, determining copper and dye resistance and decolorization abilities. Copper and dye-resistant bacteria were isolated from the Citarum River, West Java, Indonesia. Bacterial isolates were identified based on 16S rDNA gene analysis. Copper resistance was determined by measuring the minimum inhibitory concentration (MIC) of CuSO4. Dye resistance was observed by growing the bacterial isolates on a medium containing 100-500 ppm of various dyes. The dye decolorization was analyzed by monitoring the absorbance of each dye using a spectrophotometer. Fifty-four of copper indigenous resistant bacteria have been isolated. Nine bacterial isolates that showed high resistance to copper and dye with the MIC of 11 mM CuSO4 were identified as Siccibacter colletis, Acinetobacter baumannii, Lysinibacillus fusiformis, Bacillus cereus, and Escherichia coli. The highest multi-resistant bacterium was Bacillus cereus CTR 200 3.2 with decolorization rates of 93.04%, 61.9%, and 87.43% on 100 ppm methylene blue, malachite green, and basic fuchsine dye, respectively. However, adding 5 mM CuSO4 reduced those decolorization rates to 39.39%, 10.48%, and 7.39%, respectively.
Co-Authors DESTYA WATY SILALAHI DWI NINGSIH SUSILOWATI FEBRI MANGOMO MANGUNSONG INDAH SOFIANA Irawati Wahyu MICHAEL TIMOTHY REINHARD PINONTOAN REINHARD PINONTOAN SAMUEL EMMANUEL SOENTORO VALENTINE LINDARTO VALENTINE LINDARTO WAHYU IRAWATI