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All Journal Biodiversitas Journal of Biological Diversity
MUSTOFA HELMI EFFENDI
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Mulyorejo, Kampus C FKH-UNAIR, Surabaya 60115, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Antibiotic resistance pattern of Extended-Spectrum ?-Lactamase (ESBL) producing Escherichia coli isolated from broiler farm environment in Pasuruan district, Indonesia SHEILA MARTY YANESTRIA; FIDI NUR AINI EKA PUJI DAMEANTI; BETI GISTAWATI MUSAYANNAH; JUNIANTO WIKA ADI PRATAMA; ADIANA MUTAMSARI WITANINGRUM; MUSTOFA HELMI EFFENDI; EMMANUEL NNABUIKE UGBO
Biodiversitas Journal of Biological Diversity Vol. 23 No. 9 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230911

Abstract

Abstract. Yanestria SM, Dameanti FNAEP, Musayannah BG, Pratama JWA, Witaningrum AM, Effendi MH, Ugbo EN. 2022. Antibiotic resistance pattern of Extended-Spectrum ?-Lactamase (ESBL) producing Escherichia coli isolated from broiler farm environment in Pasuruan district, Indonesia. Biodiversitas 23: 4460-4465. Escherichia coli is one of the bacteria that can be used as an indicator of environmental pollution. This bacterium has the ability to become an antimicrobial-resistant bacterium that impacts public health. The antimicrobial ability can develop into a bacterium that produces extended-spectrum ?-lactamase (ESBL). The purpose of this study was to reveal the occurrence of extended-spectrum ?-lactamase (ESBL) producing E. coli from a broiler farm environment in Pasuruan district and to describe the phenotypic pattern of E. coli producing ESBL that has been detected. A total of 175 samples were used in this study consisting of 115 samples of coop swabs and 65 samples of wastewater around the farm. The samples were isolated and identified to find E. coli by using different culture media viz. McConkey agar (MCA), eosin methylene blue agar (EMBA), Gram staining, indole test, methyl red Voges Proskauer (MR-VP), citrate, and triple sugar iron agar (TSIA). Detection of ESBL using the double disc synergy test (DDST) according to standard Clinical and Laboratory Standards Institute procedures and the VITEK®2 compact apparatus. The results of ESBL confirmation with DDST and VITEK®2 showed that 16 (9.14%) of the 175 environmental samples confirmed E. coli produced ESBL. The results of the VITEK®2 test also produced a phenotypic pattern of resistance properties of ESBL-producing E. coli and found 12 types of resistance patterns. The combination of “AM AMP ATM KZ CTX CRO CIP” and “AM AMP ATM KZ CTX CRO GM CIP SXT” are the 2 most common resistance patterns (18.75%), while the other 10 resistance patterns occur at the same level (6.25%). The data presented here confirmed the presence of ESBL-producing E. coli in the farm environment, which can contribute to the dissemination of MDR bacteria in the environment if not monitored. Therefore, the presence of ESBL-producing E. coli in Pasuruan is worrisome since it can lead to an impact on human health.
Poultry slaughterhouse wastewater as reservoirs for spreading extended-spectrum beta-lactamase-producing Escherichia coli in Abakaliki, Nigeria EMMANUEL NNABUIKE UGBO; JENNIFER INIOBONG JACOB; MUSTOFA HELMI EFFENDI; ADIANA MUTAMSARI WITANINGRUM; BERNARD NNABUIFE AGUMAH; AGATHA IFUNANYA UGBO; BENJAMIN IKECHUKWU MOSES
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240939

Abstract

Abstract. Ugbo EN, Jacob JI, Effendi MH, Witaningrum AM, Agumah BN, Ugbo AI, Moses BI. 2023. Poultry slaughterhouse wastewater as reservoirs for spreading extended-spectrum beta-lactamase-producing Escherichia coli in Abakaliki, Nigeria. Biodiversitas 24: 4960-4966. The emergence of antimicrobial and multi-resistance extended-spectrum beta-lactamase-producing organisms (ESBL) from the environment and hospitals is a serious global health concern. This research focused on the role of poultry slaughterhouse wastewater as a reservoir for spreading extended-spectrum beta-lactamase-producing Escherichia coli. Exactly 110 poultry slaughterhouses wastewater was collected using sterile universal containers from abattoirs in Abakaliki. The samples were analyzed microbiological for the presence of E. coli using standard techniques. The E. coli isolates found were subjected to a sensitivity test to antibiotics using the Kirby-Bauer disc diffusion method and further tested for ESBL-producing E. coli using a double disc synergy test and chromogenic media Brilliance ESBL agar. Of 110 wastewater samples analyzed, 55 (50.0%) were positive for E. coli contamination. The E. coli isolates showed antibiotic resistance ranging between 21.8% (amoxicillin clavulanic acid) to 69.1% (tetracycline), and good susceptibility was observed on cefepime (72.7%). Thus, among 55 E. coli isolates, 11(20.0%) were ESBL-producing E. coli with resistance patterns as follows: TET-STX-CAZ-CTX-FEP (four isolates); TET-STX-CAZ-CTX (four isolates); TET-STX-CAZ-CTX-FEP-CRO (three isolates), and average multidrug resistance index of 0.7. This study revealed that poultry slaughterhouse wastewater was a reservoir for multidrug resistance ESBL-producing E. coli. Therefore, properly treating wastewater from poultry production or farms before disposing into the drainage channel or water bodies is highly recommended to avoid spreading ESBL-producing organisms from animal waste products to humans and their environment.
Antimicrobial resistance pattern of Salmonella spp. isolated from poultry farms in Abakaliki, Nigeria EMMANUEL NNABUIKE UGBO; MUSTOFA HELMI EFFENDI; ADIANA MUTAMSARI WITANINGRUM; WIWIEK TYASNINGSIH; BERNARD NNABUIFE AGUMAH; AGATHA IFUNANYA UGBO; CHARITY CHINYERE NNABUGWU; DIVINEGIFT OLUCHI OKATA-NWALI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240965

Abstract

Abstract. Ugbo EN, Effendi MH, Witaningrum AM, Tyasningsih W, Agumah BN, Ugbo AI, Nnabugwu CC, Okata-Nwali DO. 2023. Antimicrobial resistance pattern of Salmonella spp. isolated from poultry farms in Abakaliki, Nigeria. Biodiversitas 24: 5207-5214. Salmonella is a zoonotic foodborne pathogen that can cause serious illness in humans and animals worldwide. Antimicrobial resistance caused by microorganisms has greatly challenged veterinary and human medicine regarding disease treatments. The objective of this study was to evaluate the antimicrobial resistance pattern of Salmonella spp. isolated from poultry farms in Abakaliki, Nigeria. One hundred eighty samples (90 broiler feces; 90 broiler cloacal swabs) were collected from three (3) poultry farms in the Abakaliki metropolis. Samples were analyzed and identified for the presence of Salmonella isolates using standard microbiological and biochemical analysis. The antimicrobial sensitivity test of Salmonella isolates against the selected antibiotics was done using the Kirby-Bauer disk diffusion method on Mueller Hinton agar. The results showed 61 samples (33.9 %) were positive for Salmonella. Feces and cloacal swab samples positive for Salmonella were 46.7% and 21.1%, respectively. Multidrug resistance Salmonella (MDR) strains had an overall prevalence of samples (16.2%), i.e., 7 samples of broiler feces had 7 (16.7%) and 3 samples of cloacal swab (15.8%). Thus, all the MDR Salmonella isolates were 100% resistant to tetracycline and ampicillin. The multiple antibiotic-resistant indexes of the MDR were 0.51. This study indicated that broilers chicken and their products were potential sources of human salmonellosis and sources for transmitting MDR Salmonella in Abakaliki, Nigeria. Poultry farmers should use antibiotics in appropriate dosages in their poultry farms.
Molecular detection of iron gene on multidrug resistant avian fecal Escherichia coli isolated from broiler on traditional markets, Surabaya, Indonesia MARIANA FEBRILIANTI RESILINDA PUTRI; IRFAN ALIAS KENDEK; FRESHINTA JELLIA WIBISONO; MUSTOFA HELMI EFFENDI; DADIK RAHARDJO; WIWIEK TYASNINGSIH; EMMANUEL NNABUIKE UGBO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 12 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241207

Abstract

Abstract. Putri MFR, Kendek IA, Wibisono FJ, Effendi MH, Rahardjo D, Tyasningsih W, Ugbo EN. 2023. Molecular detection of iron gene on multidrug resistant avian fecal Escherichia coli isolated from broiler on traditional markets, Surabaya, Indonesia. Biodiversitas 24: 6454-6460. Avian Fecal Escherichia coli (AFEC) is the cause of colibacillosis, which often infects the poultry industry throughout the world. The virulence gene influences the iroN factor, which causes systemic infections in poultry. This research aimed to determine Multidrug Resistance (MDR) in AFEC obtained from traditional markets in Surabaya. There were 96 cloacal swab samples from broiler chickens. The samples were isolated and identified using culture media in the form of Eosin Methylene Blue Agar (EMBA), gram stain, Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), Simmons Citrate Agar (SCA), Voges Proskauer (MR-VP) indole and methyl test; detection of multidrug resistance using Mueller-Hinton Agar (MHA) media. The results of the isolation and identification of Escherichia coli bacteria in this study showed that 60.4% of the isolates were positive for Escherichia coli. Escherichia coli is resistant to tetracycline antibiotics by 56%, ciprofloxacin by 55%, antibiotics aztreonam 29%, kanamycin 20%, and chloramphenicol 18%. The multidrug resistance test result on Escherichia coli was 25.8%. The PCR test results for the iroN gene were 40%. Therefore, there are MDR and iroN genes in avian fecal Escherichia coli in Surabaya traditional markets; APEC with iroN gene poses the potential to affect public health.
Molecular detection of hlyF gene on multidrug resistance of avian pathogenic Escherichia coli isolated from ducks on wet markets of Surabaya, Indonesia IRFAN ALIAS KENDEK; MARIANA FEBRILIANTI RESILINDA PUTRI; FRESHINTA JELLIA WIBISONO; MUSTOFA HELMI EFFENDI; WIWIEK TYASNINGSIH; EMMANUEL NNABUIKE UGBO; NNABUIFE BERNARD AGUMAH
Biodiversitas Journal of Biological Diversity Vol. 25 No. 3 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250341

Abstract

Abstract. Kendek IA, Putri MFR, Wibisono FJ, Effendi MH, Tyasningsih W, Ugbo EN, Agumah NB. 2024. Molecular detection of hlyF gene on multidrug resistance of avian pathogenic Escherichia coli isolated from ducks on wet markets of Surabaya, Indonesia. Biodiversitas 25: 1246-1252. Avian Pathogenic Escherichia coli (APEC) is a strain of pathogen that can cause colibacillosis in poultry, including ducks worldwide. This disease can be influenced by various virulence genes, one of which is the hlyF gene which can cause systemic disorders and potentially become zoonotic in poultry. This study aimed to detect the hlyF virulence gene from the multidrug resistance (MDR) properties of Escherichia coli originating from 158 duck cloacal swab samples from seven live markets in Surabaya. Samples were isolated and identified using Eosin Methylene Blue Agar (EMBA) and MacConkey Agar (MCA) media, Gram staining, and then continued with biochemical tests of Triple Sugar Iron Agar (TSIA), Simmons Citrate Agar (SCA), Sulfide Indole Motility (SIM), Methyl red (MR) and Voges-Proskauer (VP). Samples were tested for MDR properties and continued with confirmation of the hlyF virulence gene using PCR. Based on the results of this study, it showed that 85% (134/158) were positive for Escherichia coli. Escherichia coli bacteria experienced resistance to the antibiotic erythromycin 96% (129/134), ciprofloxacin 16% (22/134), antibiotics gentamicin 15% (20/134), aztreonam 4.4% (6/134) and chloramphenicol 3% (5/58). The MDR test results in this study were 15% (20/134), while the PCR test results for the hlyF gene were 60% (12/20). Therefore, Escherichia coli was found to have the hlyF virulence gene, an MDR in Escherichia coli bacteria at the Surabaya live market. Furthermore, APEC strains with the hlyF gene, an MDR, potentially affect public health.
Co-Authors ADIANA MUTAMSARI WITANINGRUM AGATHA IFUNANYA UGBO AGATHA IFUNANYA UGBO BENJAMIN IKECHUKWU MOSES BERNARD NNABUIFE AGUMAH BERNARD NNABUIFE AGUMAH BETI GISTAWATI MUSAYANNAH CHARITY CHINYERE NNABUGWU DADIK RAHARDJO DIVINEGIFT OLUCHI OKATA-NWALI EMMANUEL NNABUIKE UGBO EMMANUEL NNABUIKE UGBO EMMANUEL NNABUIKE UGBO EMMANUEL NNABUIKE UGBO EMMANUEL NNABUIKE UGBO FIDI NUR AINI EKA PUJI DAMEANTI FRESHINTA JELLIA WIBISONO FRESHINTA JELLIA WIBISONO IRFAN ALIAS KENDEK JENNIFER INIOBONG JACOB JUNIANTO WIKA ADI PRATAMA MARIANA FEBRILIANTI RESILINDA PUTRI NNABUIFE BERNARD AGUMAH SHEILA MARTY YANESTRIA WIWIEK TYASNINGSIH WIWIEK TYASNINGSIH