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All Journal Biodiversitas Journal of Biological Diversity
Subagus Wahyuono
Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada. Jl. Sekip Utara, Sleman 55281, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Effect of light exposure on secondary metabolites production of an endophytic fungus Arthrinium rasikravindrae and its antioxidant and anticancer activities Andita Eltivitasari; Rahmawati Rahmawati; Baiq Maylinda Gemantari; Fitra Romadhonsyah; Arief Nurrochmad; Subagus Wahyuono; Puji Astuti
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220618

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Abstract. Eltivitasari A, Rahmawati, Gemantari BM, Romadhonsyah F, Nurrochmad A, Wahyuono S, Astuti P. 2021. Effect of light exposure on secondary metabolites production of an endophytic fungus Arthrinium rasikravindrae and its antioxidant and anticancer activities. Biodiversitas 22: 3156-3163. Endophytic microorganisms are one of the promising sources in producing bioactive compounds, to be developed for new drug candidates. They are found to have the ability to generate the same compounds as their host plant. Metabolite producing capacity of the endophytes is known to be affected by light exposure during fermentation process. This study focused on an endophytic fungus Arthrinium rasikravindrae isolated from Coleus amboinicus stem to reveal out its metabolite profiles due to light exposure as well as its bioactivity consequences. A. rasikravindrae was cultured on potato dextrose broth medium for 14 days and fermented in dark and exposed to natural light. Metabolite profiling was performed using TLC and GC-MS analysis. The activities were observed using DPPH assay for antioxidant and MTT assay for cytotoxicity potential. The results showed that A. rasikravindrae ethyl acetate extract produced during dark and exposed to light fermentation conditions contained different compounds but there was some which showed similarity with their host plant. Methyl octadec-9-enoate was found in all fermentation conditions as well as in C. amboinicus stem extract. Besides methyl octadec-9-enoate, methyl palmitate was also found present in both A. rasikravindrae extract fermented exposed to light and its host plant. The antioxidant activity of extract generated from dark fermentation condition was better as compared to that exposed to light with IC50 value of 66.36±0.53 vs 556.92±34.37 µg/mL. However, cytotoxic activity screening against several cancer cell lines exhibited opposing results in which extract from light-exposed fermentation resulted in better cytotoxic activity (IC50 value of 291.40 ± 2.34 µg/mL on WiDr, 336.80 ± 5.05 µg/mL on T47D, and 404.73 ± 3.46 µg/mL on Hela cell lines). Extract obtained from dark fermentation condition showed IC50 value of more than 500 µg/mL in all tested cancer cell lines. Preliminary examination on cytotoxic activity against WiDR cells suggested that the extract from light-exposed fermentation might induce cell death through mechanisms involving cell cycle arrest.
Screening of selected Indonesian plants for antiplatelet activity Nanang Fakhrudin; Fatiya Farih Mufinnah; Muhammad Faishal Husni; Arief Eka Wardana; Eradhian Irma Wulandari; Arbie Ristanto Putra; Djoko Santosa; Arief Nurrochmad; Subagus Wahyuono
Biodiversitas Journal of Biological Diversity Vol. 22 No. 12 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d221205

Abstract

Abstract. Fakhrudin N, Mufinnah FF, Husni MF, Wardana AE, Wulandari EI, Putra AR, Santoso D, Nurrichmad A, Wahyunono S. 2021. Screening of selected Indonesian plants for antiplatelet activity. Biodiversitas 22: 5268-5273. Cardiovascular diseases remain the leading cause of death worldwide. Platelet aggregation plays a crucial role in the development of cardiovascular diseases. Thus, the discovery of bioactive compounds targeting platelet aggregation is a promising approach for combating cardiovascular diseases. Indonesia has plants diversity with the potential to be developed as antiplatelet agents. Thus, this study was aimed to identify the antiplatelet potential of selected Indonesian plant extracts. The plant extracts (100 µg mL-1) were evaluated for their antiplatelet activity in CaCl2-induced platelet aggregation in 96-well plates. In an aggregometer, the active extracts were further evaluated in adenosine diphosphate (ADP)-induced platelet aggregation. The antiplatelet agents, aspirin, and ticagrelor were used as reference drugs in the respective screening assays. Of the 139 tested extracts, ten demonstrated antiplatelet activity in CaCl2-induced platelet aggregation. The evaluation of the ten active extracts in ADP-induced platelet aggregation revealed four active extracts. The most active extract was the methanol extract of Cinnamomum sintoc bark, followed by the methanol extract of Leea aequata leaf, and the dichloromethane and methanol extracts of Physalis angulata petal with moderate activities. This study provides essential scientific evidence for the development of antiplatelet agents from plant origins.
Co-Authors Andita Eltivitasari Arbie Ristanto Putra Arief Nurrochmad Baiq Maylinda Gemantari Djoko Santosa Eradhian Irma Wulandari Fatiya Farih Mufinnah Fitra Romadhonsyah Muhammad Faishal Husni Nanang Fakhrudin Puji Astuti Rahmawati Rahmawati