Article Per Year (5 Year)
p-Index From 2021 - 2026
0.817
P-Index
This Author published in this journals
All Journal Biodiversitas Journal of Biological Diversity
Puji Astuti
Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada. Jl. Sekip Utara, Sleman 55281, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
Effect of light exposure on secondary metabolites production of an endophytic fungus Arthrinium rasikravindrae and its antioxidant and anticancer activities Andita Eltivitasari; Rahmawati Rahmawati; Baiq Maylinda Gemantari; Fitra Romadhonsyah; Arief Nurrochmad; Subagus Wahyuono; Puji Astuti
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220618

Abstract

Abstract. Eltivitasari A, Rahmawati, Gemantari BM, Romadhonsyah F, Nurrochmad A, Wahyuono S, Astuti P. 2021. Effect of light exposure on secondary metabolites production of an endophytic fungus Arthrinium rasikravindrae and its antioxidant and anticancer activities. Biodiversitas 22: 3156-3163. Endophytic microorganisms are one of the promising sources in producing bioactive compounds, to be developed for new drug candidates. They are found to have the ability to generate the same compounds as their host plant. Metabolite producing capacity of the endophytes is known to be affected by light exposure during fermentation process. This study focused on an endophytic fungus Arthrinium rasikravindrae isolated from Coleus amboinicus stem to reveal out its metabolite profiles due to light exposure as well as its bioactivity consequences. A. rasikravindrae was cultured on potato dextrose broth medium for 14 days and fermented in dark and exposed to natural light. Metabolite profiling was performed using TLC and GC-MS analysis. The activities were observed using DPPH assay for antioxidant and MTT assay for cytotoxicity potential. The results showed that A. rasikravindrae ethyl acetate extract produced during dark and exposed to light fermentation conditions contained different compounds but there was some which showed similarity with their host plant. Methyl octadec-9-enoate was found in all fermentation conditions as well as in C. amboinicus stem extract. Besides methyl octadec-9-enoate, methyl palmitate was also found present in both A. rasikravindrae extract fermented exposed to light and its host plant. The antioxidant activity of extract generated from dark fermentation condition was better as compared to that exposed to light with IC50 value of 66.36±0.53 vs 556.92±34.37 µg/mL. However, cytotoxic activity screening against several cancer cell lines exhibited opposing results in which extract from light-exposed fermentation resulted in better cytotoxic activity (IC50 value of 291.40 ± 2.34 µg/mL on WiDr, 336.80 ± 5.05 µg/mL on T47D, and 404.73 ± 3.46 µg/mL on Hela cell lines). Extract obtained from dark fermentation condition showed IC50 value of more than 500 µg/mL in all tested cancer cell lines. Preliminary examination on cytotoxic activity against WiDR cells suggested that the extract from light-exposed fermentation might induce cell death through mechanisms involving cell cycle arrest.
Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines Khusnul Fadhilah; Subagus Wahyuono; Puji Astuti
Biodiversitas Journal of Biological Diversity Vol. 22 No. 9 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220918

Abstract

Abstract. Fadhilah K, Wahyuono S, Astuti P. 2021. Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines. Biodiversitas 22: 3743-3748. Lansium domesticum (Fam. Meliaceae), a tropical fruit (local name, Duku), has been reported to have various biological activities. At the moment we emphasize on searching compounds to have cytotoxic activity from the peel of L. domesticum. The study was initiated by extraction with EtOAc followed by fractionation that was monitored by thin-layer chromatography (TLC) and cytotoxicity against T-47D and HepG2 cells. The EtOAc extract of the sample was triturated with n-hexane to give n-hexane soluble (A) n-hexane insoluble fractions (B). The B fraction was fractionated by vacuum column chromatography using gradient solvent composition of n-hexane: acetone to give 18 fractions. According to TLC similarity pictures, fractions were combined to give 6 fractions (I-VI). Isolation was performed using preparative TLC, and the cytotoxic assay was performed using MTT method. The isolated compound was identified as a sesquiterpene having one aldehyde functional group, based on spectroscopic data.The isolated compound displayed cytotoxic activity on T-47D (IC50, 48.58 + 0.96 µg/mL) and HepG2 (IC50, 127.45 + 25.76 µg/mL).
Short Communication: Terpenoids isolated from Lansium domesticum fruit peel induce apoptosis and cell cycle arrest in T-47D cell lines KHUSNUL FADHILAH; SUBAGUS WAHYUONO; PUJI ASTUTI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 2 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230262

Abstract

Abstract. Fadhilah K, Wahyuono S, Astuti P. 2022. Short Communication: Terpenoids isolated from Lansium domesticum fruit peel induce apoptosis and cell cycle arrest in T-47D cell lines. Biodiversitas 23: 1167-1171. One of the characteristics of cancer cells is uncontrolled proliferation. This can be caused by aberrant in the proteins that play a role in the process of apoptosis and cell cycle. Lamesticumin A and 2-ethyl,3-(1’-hydroxy-2’-menthene) propenal have been isolated from ethyl acetate extract of L. domesticum fruit peel had cytotoxicity against T-47D cells. However, the study on how these terpenoids induce cell death is still unknown. Flow cytometry analysis was carried out to evaluate the effect of the compounds in cell cycle modulation and apoptotic induction. The terpenoid compounds induced apoptosis and cell cycle arrest in S phase in a dose dependent manner. Compound 1 synergized the effect of doxorubicin in inducing cell death through mechanism involving the cell cycle. Compound 1 revealed better activity in killing T-47D cells rather than compound 2. Further studies are needed to explore the potential of these compounds in promoting cell death of breast cancer.
Cytotoxic screening of endophytic fungi associated with Catharanthus roseus CATERINA SISKADEWI LAY; IKRA AGUSTINA; PUJI ASTUTI; TRIANA HERTIANI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 5 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240525

Abstract

Abstract. Lay CS, Agustina I, Astuti P, Hertiani T. 2023. Cytotoxic screening of endophytic fungi associated with Catharanthus roseus. Biodiversitas 24: 2716-2722. Despite the advancements in cancer therapy, there is still a need for new anticancer agents. Due to the tremendous range of chemical compounds that endophytic fungi create, they have become a crucial component of the current drug development process. The aim of this study was to evaluate the cytotoxic potential of endophytic fungi associated with Catharanthus roseus (L.) G.Don collected from Yogyakarta, Indonesia. All fungal EtOAc extracts were tested against three human cancer cell lines, HeLa, T47D, MCF-7, and Vero cell lines. The potential fungal isolate was identified using molecular techniques. The results showed that a total of seven endophytic fungi were obtained from the different parts of C. roseus. Only one fungus (SCR 3) showed the best cytotoxic activitiy towards all three cell lines used, especially the MCF-7 cell line (IC50 130.90 ?g/mL, Selectivity Index=11.42). The findings of using LC-HRMS also revealed several exciting compounds such as genistein and D-(-)-Mannitol. The results showed that EtOAc extract of SCR 3 (Aspergillus sp.) induced apoptosis but did not affect the cell cycle. This study suggests that EtOAc extract of SCR 3 may be a potential source of secondary metabolites that can function as a new source of anticancer agents.
Co-Authors Andita Eltivitasari Arief Nurrochmad Baiq Maylinda Gemantari CATERINA SISKADEWI LAY Fitra Romadhonsyah IKRA AGUSTINA Khusnul Fadhilah Rahmawati Rahmawati Subagus Wahyuono Subagus Wahyuono TRIANA HERTIANI