Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Biodiversitas Journal of Biological Diversity
Khusnul Fadhilah
Faculty of Pharmacy, Universitas Gadjah Mada. Jl. Sekip Utara, Sleman 55281, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines Khusnul Fadhilah; Subagus Wahyuono; Puji Astuti
Biodiversitas Journal of Biological Diversity Vol. 22 No. 9 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220918

Abstract

Abstract. Fadhilah K, Wahyuono S, Astuti P. 2021. Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines. Biodiversitas 22: 3743-3748. Lansium domesticum (Fam. Meliaceae), a tropical fruit (local name, Duku), has been reported to have various biological activities. At the moment we emphasize on searching compounds to have cytotoxic activity from the peel of L. domesticum. The study was initiated by extraction with EtOAc followed by fractionation that was monitored by thin-layer chromatography (TLC) and cytotoxicity against T-47D and HepG2 cells. The EtOAc extract of the sample was triturated with n-hexane to give n-hexane soluble (A) n-hexane insoluble fractions (B). The B fraction was fractionated by vacuum column chromatography using gradient solvent composition of n-hexane: acetone to give 18 fractions. According to TLC similarity pictures, fractions were combined to give 6 fractions (I-VI). Isolation was performed using preparative TLC, and the cytotoxic assay was performed using MTT method. The isolated compound was identified as a sesquiterpene having one aldehyde functional group, based on spectroscopic data.The isolated compound displayed cytotoxic activity on T-47D (IC50, 48.58 + 0.96 µg/mL) and HepG2 (IC50, 127.45 + 25.76 µg/mL).
Short Communication: Terpenoids isolated from Lansium domesticum fruit peel induce apoptosis and cell cycle arrest in T-47D cell lines KHUSNUL FADHILAH; SUBAGUS WAHYUONO; PUJI ASTUTI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 2 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230262

Abstract

Abstract. Fadhilah K, Wahyuono S, Astuti P. 2022. Short Communication: Terpenoids isolated from Lansium domesticum fruit peel induce apoptosis and cell cycle arrest in T-47D cell lines. Biodiversitas 23: 1167-1171. One of the characteristics of cancer cells is uncontrolled proliferation. This can be caused by aberrant in the proteins that play a role in the process of apoptosis and cell cycle. Lamesticumin A and 2-ethyl,3-(1’-hydroxy-2’-menthene) propenal have been isolated from ethyl acetate extract of L. domesticum fruit peel had cytotoxicity against T-47D cells. However, the study on how these terpenoids induce cell death is still unknown. Flow cytometry analysis was carried out to evaluate the effect of the compounds in cell cycle modulation and apoptotic induction. The terpenoid compounds induced apoptosis and cell cycle arrest in S phase in a dose dependent manner. Compound 1 synergized the effect of doxorubicin in inducing cell death through mechanism involving the cell cycle. Compound 1 revealed better activity in killing T-47D cells rather than compound 2. Further studies are needed to explore the potential of these compounds in promoting cell death of breast cancer.
Co-Authors Puji Astuti Subagus Wahyuono