Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Biodiversitas Journal of Biological Diversity
AHMAD YUNUS
Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta 57126, Central Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Fruit morphological diversity and quality of pulasan (Nephelium ramboutan-ake) from six populations in Riau, Indonesia MARDALENI MARDALENI; HASAN BASRI JUMIN; NANDARIYAH NANDARIYAH; AHMAD YUNUS
Biodiversitas Journal of Biological Diversity Vol. 23 No. 5 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230533

Abstract

Abstract. Mardaleni, Jumin HB, Nandariyah, Yunus A. 2022. Fruit morphological diversity and quality of pulasan (Nephelium ramboutan-ake) from six populations in Riau, Indonesia. Biodiversitas 23: 2526-2533. Pulasan (Nephelium ramboutan-ake), which belongs to the Sapindaceae family, has economic value, but information on the morphological diversity and quality of its fruit is unknown. Thus, this study aimed to characterize the morphology and quality of pulasan fruits from six populations in Riau Province, Indonesia and observe the kinship among populations. A total of 18 qualitative and 13 quantitative characteristics were evaluated on 67 pulasan cultivars. The difference among morphological characteristics was to analysed using ANOVA and Duncan multiple range tests, while the kinship analysis used UPGMA. Results of the quantitative and qualitative characterization of pulasan fruits showed high diversity among populations. The fruit is oblong, and it has a uniform skin color, sweet taste, aril cream-white color, and aril that can be easily peeled from the seeds. These characteristics indicate good fruit quality. Dendrogram analysis divided the population into two large groups at 73% similarity. The first group consisted of the population of Kampar Kiri, Gunung Sahilan, and Kuok, whereas the second group consisted of the population of Bengkalis, Siak, and Kuansing. The results of this study provide baseline information to obtain potentially superior characteristics for breeding and conservation of genetic resources, which can be used as a basis for the conservation of the germplasm of pulasan.
Construction of the CRISPR/Cas9 module and genetic transformation of aromatic rice cv. Mentik Wangi for developing bacterial leaf blight resistance NURUL FARIDAH RIFHANI; ANIVERSARI APRIANA; ATMITRI SISHARMINI; TRI JOKO SANTOSO; KURNIAWAN RUDI TRIJATMIKO; INEZ H. SLAMET-LOEDIN; AHMAD YUNUS
Biodiversitas Journal of Biological Diversity Vol. 24 No. 6 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240620

Abstract

Abstract. Rifhani NF, Apriana A, Sisharmini A, Santoso TJ, Trijatmiko KR, Slamet-Loedin IH, Yunus A. 2023. Construction of the CRISPR/Cas9 module and genetic transformation of aromatic rice cv. Mentik Wangi for developing bacterial leaf blight resistance. Biodiversitas 24: 3258-3268.Rice cv. Mentik Wangi is a local aromatic rice that is susceptible to pests and diseases, such as bacterial leaf blight (BLB) caused by Xanthomonas oryzae(Xoo). This bacteria can cause damage to plants thereby reducing crop yields. This study aimed to obtain the CRISPR/Cas9 module construct as well as introducing the construct into rice cv. Mentik Wangi for developing BLB resistance. The manufacture of the CRISPR/Cas9 module was carried out using the Golden Gate method, and the introduction of that construct into rice cv. Mentik Wangi was carried out using Agrobacterium tumefaciens. Constructing a CRISPR/Cas9 module with multiple gRNA of OsSWEET11 and OsSWEET14 genes has succeeded, 129 putative transformant lines of T0 generation produced with the regeneration and transformation efficiency values ??are respectively 9.4% and 9.8%. The results showed that 36 lines were positive for the hpt gene, indicating that the CRISPR/Cas9-gRNAOsSWEET module construct was successfully entered into rice cv. Mentik Wangi. Further analysis is needed to identify mutagenesis in the target gene region of TI generation transgenic plants as well as phenotypic tests for BLB resistance.
Co-Authors ANIVERSARI APRIANA ATMITRI SISHARMINI HASAN BASRI JUMIN INEZ H. SLAMET-LOEDIN KURNIAWAN RUDI TRIJATMIKO MARDALENI MARDALENI NANDARIYAH NANDARIYAH NURUL FARIDAH RIFHANI TRI JOKO SANTOSO