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All Journal Biodiversitas Journal of Biological Diversity
ARIDA SUSILOWATI
Faculty of Forestry, Universitas Sumatera Utara. Jl. Padang Bulan, Medan 20155, North Sumatra, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Molecular identification of Eurycoma longifolia Jack from Sumatra, Indonesia using trnL-F region KUSUMADEWI SRI YULITA; ARIDA SUSILOWATI; HENTI HENDALASTUTI RACHMAT; SUSILA SUSILA; ASEP HIDAYAT; FIFI GUS DWIYANTI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 3 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230323

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Abstract. Yulita KS, Susilowati A, Rachmat HH, Susila, Hidayat A, Dwiyanti FG. 2022. Molecular identification of Eurycoma longifolia Jack from Sumatra, Indonesia using trnL-F region. Biodiversitas 23: 1374-1382. Eurycoma longifolia Jack (Simaroubaceae) or pasak bumi is a popular medicinal plant from Southeast Asia’s rainforests that is used as an aphrodisiac, antimicrobial, anti-malaria antidiabetic, antiulcer, and anticancer agent. However, the increasing demand for this species for medicinal industries has led to illegal export in Indonesia. This study aimed to determine the specific genetic variation and develop DNA barcode using trnL-Fregion for E. longifolia originating from Sumatra, Indonesia. Twenty-two samples of the species were collected from four locations in Sumatra. An aligned sequence of the trnL-Fwas 960 bp with an A/T rich region (A: 30.2%, T: 34.5%, C: 16.7%, and G: 18.7%). The homology search using BLASTn of the GenBank NCBI showed that the nucleotide composition of the species was similar (99.9%) to the partial trnL-Fregion of E. longifolia MH751519 and E. apiculata GU593014. Close examination of the gene structure and composition showed that the DNA sequences have five nucleotides variations that were not possessed by the reference E. longifolia, and other taxa used. The obtained variations occurred mostly in the trnL intron region, and the phylogenetic analysis showed that the correct identity of the species of the samples by their position was at a similar clade as the other accessions of E. longifolia.
Genetic variation and phylogenetic position of Taxus sumatrana (Miq.) de Laub. (Taxaceae) in Sumatra, Indonesia based on trnL-trnF region IRSYAD KAMAL; NAWWALL ARROFAHA; SUSILA SUSILA; KUSUMADEWI SRI YULITA; HENTI HENDALASTUTI RACHMAT; ARIDA SUSILOWATI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240960

Abstract

Abstract. Kamal I, Arrofaha N, Susila, Yulita KS, Rachmat HH, Susilowati A. 2023. Genetic variation and phylogenetic position of Taxus sumatrana (Miq.) de Laub. (Taxaceae) in Sumatra, Indonesia based on trnL-trnF region. Biodiversitas 24: 5155-5166. Taxus sumatrana (Miq.) de Laub. is a synonym of T. wallichiana Zucc. by some authors based on morphological characters and its distribution. This species is a medicinal plant and contains taxol as an anti-cancer with slow natural regeneration rate and increasing threats to their existence. This study aimed to determine the genetic diversity and the phylogenetic analysis using trnL-trnF for T. sumatrana growing in Sumatra, Indonesia. The total of 37 individuals from three natural populations of T. sumatrana were studied, consisting of the current new collection from Toba - North Sumatra and previously studied population of Dempo - South Sumatra and Sungai Penuh, Kerinci Seblat National Park - Jambi. The Toba population did not show variation at individual level within the population as similar results were obtained from the previous study. However, one indel (- and A) was found at 217 bp from all three T. sumatrana populations from Sumatra, Indonesia. When the sequences were aligned with the reference sequence from GenBank, single base substitutions were found at 344, 478,722, and 757 bp consisting of one indel and three single nucleotide polymorphisms (SNPs). Phylogeny tree showed that T. sumatrana from Sumatra population clustered together with T. mairei and T. wallichiana var. mairei and later combined with other T. sumatrana available in NCBI.
Co-Authors ASEP HIDAYAT FIFI GUS DWIYANTI HENTI HENDALASTUTI RACHMAT HENTI HENDALASTUTI RACHMAT IRSYAD KAMAL KUSUMADEWI SRI YULITA KUSUMADEWI SRI YULITA NAWWALL ARROFAHA SUSILA SUSILA SUSILA SUSILA