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All Journal HAYATI Journal of Biosciences Journal of Tropical Biodiversity and Biotechnology Biodiversitas Journal of Biological Diversity
ANDI EKO MARYANTO
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Indonesia Biodiversity And Environmental Genomics Research Cluster, Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology

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Local adaptation of invasive plant, Synedrella nodiflora, in urban tropical lowland landscape Universitas Indonesia Andi Eko Maryanto; Andi Salamah; Citra Karina Windarti; Mutia Syadewi
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 3 (2021): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.64622

Abstract

Synedrella nodiflora is an invasive species originated from tropical America and now has spread throughout Indonesia. We analysed the ability of Synedrella nodiflora from the level of HSP70 gene expression at different heat stress in urban tropical lowland landscape Universitas Indonesia.  We used the qPCR to quantify the level of HSP70 gene expression and analysed using Pfaffl model. We found the level of HSP70 gene expression got higher related to elevated temperature from 29oC to 39oC with a range of fold from 123.1 to 1676.9. This ability reflects the adaptive plasticity of Synedrella nodiflora in the course of the invasion process. 
Development of DNA Extraction Method for Forensics Studies of Preserved Hair and Skin Samples from Sumatran Tiger (Panthera tigris sumatrae, Pocock 1929) Noviar Andayani; Andi Eko Maryanto; Muhammad Naufal Nur
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.816-824

Abstract

Poaching and illegal wildlife trade present severe threats to the Sumatran tiger. The high demand for tiger body parts leads to a high number of imitations in illegal markets, complicating the morphological identification of any confiscation cases. Accurate identification is essential in legal due process, given that the national protection law only regulates Indonesia's native species. Identification using molecular approaches may overcome the problem. However, most illegally traded tiger body parts have been preserved for an extended period of time, reducing the quantity and quality of the recovered DNA. This study aimed to develop a fast and effective method to recover DNA from preserved forensic samples. The methods had been tested with several museum samples of arsenic-treated hairs and a tiger skin piece obtained from the National Research and Innovation Agency (BRIN, formerly LIPI), tiger hairs obtained from Conservation of Natural Resources Agency (BKSDA) of Bengkulu Province, and a confiscated tiger skin sample from BKSDA Aceh. The DNA was extracted using ion-exchange, salting out, and protease-based methods. The results showed that the protease-based extraction outperformed the others to yield applicable DNA isolates for PCR-based species identification by Cyt b and ND2 mtDNA partial genes from preserved samples. However, further works are still needed to recover sufficient DNA yields for sex identification.
Analysis of performance and gene OsFER1 expression of six rice varieties with FeSO4 stress treatment FAUZIAH KHAIRATUNNISA; ANDI SALAMAH; ANDI EKO MARYANTO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 3 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240308

Abstract

Abstract. Khairatunnisa F, Salamah A, Maryanto AE. 2023. Analysis of performance and gene OsFER1 expression of six rice varieties with FeSO4 stress treatment. Biodiversitas 24: 1391-1399. Efforts to meet rice needs in Indonesia can be made through an extensification program. New paddy fields generally have a high FeSO4 content, so rice varieties resistant to these conditions are needed. This study aims to evaluate the growth of lowland rice varieties under FeSO4 stress and to analyze the expression of a gene that plays a role in iron storage, namely the OsFER1. Six lowland rice varieties, namely Ciherang, Inpari 42, Inpari 30, Sunggal, Logawa, and Cibogo were grown on media with 0 and 400 ppm FeSO4 applications. The plant height, leaf bronzing score, and the number of filled grains produced were observed to determine the effect of FeSO4 administration on the agronomic performance of rice. The OsFER1 gene expression ratio was analyzed using real-time PCR. The best plant height at 400 ppm FeSO4 was found in the Inpari 30 variety (40.3 ± 0.49 cm), with a leaf bronzing score was 1. The Inpari 30 also produced grain at 400 ppm FeSO4. Molecular analysis showed that the highest OsFER1 expression was found in the Inpari 30 grown at 400 ppm FeSO4. Based on morphological and molecular observations, Inpari 30 is tolerant to FeSO4 stress.
Probiotic and genomic evaluation of Lactiplantibacillus plantarum T31 isolated from tempoyak in mung bean substrate QONITA GINA FADHILAH; AUDREY ELIZABEHT; MELINDA FALENCIA; IMAN SANTOSO; ANDI EKO MARYANTO; NIARSI MERRY HEMELDA; MARDIASTUTI H. WAHID
Biodiversitas Journal of Biological Diversity Vol. 26 No. 9 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260916

Abstract

Abstract. Fadhilah QG, Elizabeht A, Falencia M, Santoso I, Maryanto AE, Hemelda NM, Wahid MH. 2025. Probiotic and genomic evaluation of Lactiplantibacillus plantarum T31 isolated from tempoyak in mung bean substrate. Biodiversitas 26: 4435-4444. Traditional fermented foods in Indonesia contain diverse beneficial microorganisms, including Lactic Acid Bacteria (LAB), which play an important role. LAB T31 has been isolated from tempoyak and evaluated for probiotic characters. In this research, the isolate was evaluated as a potential probiotic in a mung bean substrate to develop a healthy fermented drink. The result showed that LAB T31 grew well on the mung bean substrate. The population increased from 107 CFU/mL to 109 CFU/mL after 24 hours of incubation. The viability results showed that the LAB T31 population remained 10? CFU/mL after 30 days at 10°C. Cell-free filtrate of fermented mung bean substrate showed antibacterial activity against the tested bacteria. The diameter of inhibition increases from 24 hours (15-17 mm) to 48 hours (16-18 mm) of incubation. Whole genome sequences (WGS) of the isolate were performed using DNBseq technology, and the re-identification using JspeciesWS and FastANI showed that the strain belonged to Lactiplantibacillus plantarum. Analysis of the antibacterial assay test result showed that the LAB T31 genome encoded an antimicrobial peptide known as plantaricin. The hydrophobicity and auto-aggregation reached 38.86% and 36.92%, respectively, after 24 hours of incubation. Functional annotation reported adhesin genes, and the isolate did not suggest hemolytic activity on blood agar. These results supported WGS analysis using KEGG since the reported genes are not related to hemolytic ability and virulence factors. Moreover, antimicrobial resistance analysis showed that the isolate had a vanY gene, with a similarity level of 33.33%. LAB T31 was a potential isolate as a probiotic agent in the mung bean substrate.
Co-Authors Andayani, Noviar Andi Salamah AUDREY ELIZABEHT Citra Karina Windarti FAUZIAH KHAIRATUNNISA IMAN SANTOSO Mardiastuti Mardiastuti MELINDA FALENCIA Muhammad Naufal Nur Mutia Syadewi Niarsi Merry Hemelda QONITA GINA FADHILAH