Garuda - Garba Rujukan Digital

The Development of Gametophyte Sterilization Method for Liverworts Acrolejeunea fertilis (Reinw., Blume and Nees) Schiffn. In vitro Culture Mouleidi Dwi Putri; Windri Handayani; Astari Dwiranti; Andi Salamah; Niarsi Merry Hemelda; Afiatry Putrika
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.39

In vitro culture gametophytes of leafy liverworts often have problems in their sterilization process. These problems due to the high level of contamination and the fragile structure of the gametophyte leafy liverworts. The structures can be easily to damage after exposure to disinfectant. This study aimed to observe the concentration and the exposure time of “Bayclin” commercial bleach to suppress contamination with the viability of Acrolejeunea fertilisgametophytic explants. This research was conducted using control and 6 combination treatments with “Bayclin” concentration (1.00%, 1.25%, and 1.50%) and exposure time 60 and 120 seconds, then accompanied by the addition of Tetracycline 2.5 mg/ml. The qualitative parameters observed were the explant color, the type and location of contamination, and the growth of explants. The quantitative parameters were the percentage of contamination, the percentage of growth, and the number of new branches. The results showed that “Bayclin” 1.25% and 1.50% with 60 seconds exposure time has the lowest percentage of contamination which is 70% until the 7th days after planting. The most common type of internal contamination from the explant is bacteria and fungi. However, the growth of the new branch still occurs in some explants even though it has been contaminated and browned.

The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp. Anna Widyastuti; Afiatry Putrika; Astari Dwiranti; Andi Salamah; Niarsi Merry Hemelda; Windri Handayani
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.110

In vitro cultures of leafy liverworts are still facing significant challenges due to high-level of explant contamination. The sterilization process can easily damage the structure of liverwort after exposure to the disinfectant. This study was to determine the concentration and time exposure of commercial bleach as a disinfectant to suppress contamination using the gametophyte culture of Lopholejeunea sp. The experiment consisted of control and six treatment combinations of commercial bleach with concentration 0.5, 0.75, and 1% (v/v), and exposure time (60 and 90 seconds). The type and location of contamination, the color of the explants after sterilization, and response after 30 days were observed. The results showed that the 0.75% bleach with 60 and 90 seconds exposure time had a lower contamination until the 7th day of culture. The most common type of contamination is bacteria and fungi that arise from the explant. Despite the contamination, it did not inhibit shoot formation. Further studies still needed to determine the type of fungicides and antibiotics with the most potent concentration and exposure time should be tested to obtain an axenic and viable culture of liverworts Lopholejeunea sp.

VARIASI MORFOLOGI Calamus javensis Blume DI TAMAN NASIONAL GUNUNG HALIMUN-SALAK (TNGHS) Niarsi Merry Hemelda; Mega Atria; Noviar Andayani
BIOTIKA Jurnal Ilmiah Biologi Vol 16, No 2 (2018): BIOTIKA DESEMBER 2018
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v16i2.19832

Karakteristik Lumut di Ruang Terbuka Hijau (RTH) di Area Permukiman Jakarta Selatan Sarah Tsabituddinillah; Afiatry Putrika; Niarsi Merry Hemelda; Andi Salamah; Windri Handayani; Astari Dwiranti; Mega Atria
Al-Kauniyah: Jurnal Biologi Vol 16, No 1 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i1.21811

AbstrakPermukiman merupakan salah satu ruang terbuka hijau (RTH) yang terdapat di daerah urban, khususnya Jakarta. Salah satu kelompok tumbuhan yang ditemui pada RTH tersebut adalah lumut. Keberadaan lumut di permukiman urban menunjukkan adanya kemampuan lumut untuk bertahan pada lingkungan yang terganggu. Penelitian ini bertujuan untuk mengetahui spesies lumut serta karakteristik lumut di salah satu permukiman Jakarta Selatan. Lumut dikoleksi dengan metode transect-line pada 6 titik tepi jalan dan jelajah bebas pada 3 taman di permukiman tersebut Jakarta Selatan. Pengamatan karakteristik morfologi dan anatomi lumut dilakukan dengan penilaian kualitatif dan kuantitatif. Berdasarkan hasil penelitian terdapat 2 divisi lumut, yaitu Bryophyta (lumut sejati) dan Marchantiophyta (lumut hati) di lokasi penelitian. Bryophyta terdiri dari 6 famili, 9 genus, dan 16 spesies. Sementara itu, Marchantiophyta terdiri dari 2 famili, 2 genus, dan 3 spesies. Pottiaceae merupakan famili dengan jumlah spesies terbanyak ditemukan, yaitu 5 spesies. Fissidens biformis adalah spesies dengan jumlah sampel terbanyak. Lumut tersebut ditemukan pada substrat tanah, batu, dan batang pohon. Kisaran luas tutupan lumut yang ditemukan yaitu 2–100%. Karakteristik seperti ukuran tubuh yang kecil, bentuk hidup, bentuk daun, ornamentasi pada permukaan daun, modifikasi sel daun, serta keberadaan sporofit atau gemma diduga mendukung lumut beradaptasi di lingkungan urban.AbstractSettlement is one of urban green open spaces in Jakarta. One of the plant groups found in the open green spaces is the bryophytes. The presence of bryophytes in the settlement areas indicates the ability of bryophytes to survive in a disturbed environment. This study aims to determine bryophytes species and their characteristic in the settlements area of South Jakarta. Bryophyte collected by transect-line at 6 sites of roadside and broad survey at 3 sites of park. The morphological and anatomical characteristics were observed with qualitative and quantitative assessments. Mosses and liverworts are groups that found in study sites. The mosses consists of 6 families, 9 genera, and 16 species. Meanwhile, the liverworts consists of 2 families, 2 genera, and 3 species. Pottiaceae is has the highest species richness in the location. Meanwhile the highest number of samples was Fissidens biformis. The bryophytes were attached in the soil, rock, and tree trunk. The coverage of bryophyte is about 2–100%. Characteristics such as small body size, life-forms, leaf shape, the ornamentation on the leaf surface, modified leaf cells, and the presence of sporophyte or gemmae are thought to support the adaptation of bryophyte in urban environments.

Karakteristik Lumut di Ruang Terbuka Hijau (RTH) di Area Permukiman Jakarta Selatan Sarah Tsabituddinillah; Afiatry Putrika; Niarsi Merry Hemelda; Andi Salamah; Windri Handayani; Astari Dwiranti; Mega Atria
Al-Kauniyah: Jurnal Biologi Vol 16, No 1 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i1.21811

AbstrakPermukiman merupakan salah satu ruang terbuka hijau (RTH) yang terdapat di daerah urban, khususnya Jakarta. Salah satu kelompok tumbuhan yang ditemui pada RTH tersebut adalah lumut. Keberadaan lumut di permukiman urban menunjukkan adanya kemampuan lumut untuk bertahan pada lingkungan yang terganggu. Penelitian ini bertujuan untuk mengetahui spesies lumut serta karakteristik lumut di salah satu permukiman Jakarta Selatan. Lumut dikoleksi dengan metode transect-line pada 6 titik tepi jalan dan jelajah bebas pada 3 taman di permukiman tersebut Jakarta Selatan. Pengamatan karakteristik morfologi dan anatomi lumut dilakukan dengan penilaian kualitatif dan kuantitatif. Berdasarkan hasil penelitian terdapat 2 divisi lumut, yaitu Bryophyta (lumut sejati) dan Marchantiophyta (lumut hati) di lokasi penelitian. Bryophyta terdiri dari 6 famili, 9 genus, dan 16 spesies. Sementara itu, Marchantiophyta terdiri dari 2 famili, 2 genus, dan 3 spesies. Pottiaceae merupakan famili dengan jumlah spesies terbanyak ditemukan, yaitu 5 spesies. Fissidens biformis adalah spesies dengan jumlah sampel terbanyak. Lumut tersebut ditemukan pada substrat tanah, batu, dan batang pohon. Kisaran luas tutupan lumut yang ditemukan yaitu 2–100%. Karakteristik seperti ukuran tubuh yang kecil, bentuk hidup, bentuk daun, ornamentasi pada permukaan daun, modifikasi sel daun, serta keberadaan sporofit atau gemma diduga mendukung lumut beradaptasi di lingkungan urban.AbstractSettlement is one of urban green open spaces in Jakarta. One of the plant groups found in the open green spaces is the bryophytes. The presence of bryophytes in the settlement areas indicates the ability of bryophytes to survive in a disturbed environment. This study aims to determine bryophytes species and their characteristic in the settlements area of South Jakarta. Bryophyte collected by transect-line at 6 sites of roadside and broad survey at 3 sites of park. The morphological and anatomical characteristics were observed with qualitative and quantitative assessments. Mosses and liverworts are groups that found in study sites. The mosses consists of 6 families, 9 genera, and 16 species. Meanwhile, the liverworts consists of 2 families, 2 genera, and 3 species. Pottiaceae is has the highest species richness in the location. Meanwhile the highest number of samples was Fissidens biformis. The bryophytes were attached in the soil, rock, and tree trunk. The coverage of bryophyte is about 2–100%. Characteristics such as small body size, life-forms, leaf shape, the ornamentation on the leaf surface, modified leaf cells, and the presence of sporophyte or gemmae are thought to support the adaptation of bryophyte in urban environments.

The capacity of trees to remove particulate matter and lead and its impact on tree health RATNA YUNIATI; WINDRI HANDAYANI; LAELIA NUGRAHANI; ITA KHOERUNNISA; HALIMAH HALIMAH; AFIATRY PUTRIKA; NIARSI MERRY HEMELDA
Biodiversitas Journal of Biological Diversity Vol. 25 No. 8 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250826

Abstract. Yuniati R, Handayani W, Nugrahani L, Khoerunnisa I, Halimah, Putrika A, Hemelda NM. 2024. The capacity of trees to remove particulate matter and lead and its impact on tree health. Biodiversitas 25: 2541-2551. In urban areas, common air pollutants include particulate matter (PM) and lead (Pb) particles. Plants offer a solution to mitigate both PM and Pb particles. However, PM and Pb deposition in plant leaf organs can have an impact on tree health, which can be evaluated from plant physiological aspects. A study was conducted to compare the capacity of several plant species to absorb PM and lead particles and to determine the impact of these pollutants on the physiological aspects of plants in two locations. The selected plant species were Cerbera odollam, Polyalthia longifolia, Swietenia macrophylla, and Terminalia mantaly. The four species were chosen based on their occurrence in two distinct locations: the Universitas Indonesia (UI) campus, which represents a low-pollution area, and the Bantargebang waste landfill, which represents a highly polluted area. The method employed involves using the Gravimetric Method and observing leaf morphology to evaluate the ability to capture PM, analyzing heavy metal accumulation to assess Pb particle adsorption, and examining leaf anatomy and physiology to determine tree health. Physiological aspects were observed, including chlorophyll content, carotenoid content, relative water content (RWC), and pH of leaf extract. The results showed that the highest PM deposit on plants leaf was observed in P. longifolia, followed by S. macrophylla, C. odollam, and T. mantaly. Pb analysis revealed the order of plants that accumulate Pb, from highest to lowest, is S. macrophylla>P. longifolia>C. odollam. The PM deposits significantly affected RWC (r=-0.522, p<0.01) and it affected the content of chlorophyll (r=-0.28) and carotenoids (r=-0.017). Of all the plants studied, S. macrophylla was the most impacted, with two physiological aspects affected: pigments (chlorophyll and carotenoids), and RWC. The selection of the four tree species as pollutant absorbers in Bantargebang landfill and UI Campus is appropriate, as tree health indicators, based on the analysis of leaf anatomy and physiological characteristics, detect minimal impact from environmental stress. Polyalthia longifolia (mast tree) would be the best performer among all the tree species.

Probiotic and genomic evaluation of Lactiplantibacillus plantarum T31 isolated from tempoyak in mung bean substrate QONITA GINA FADHILAH; AUDREY ELIZABEHT; MELINDA FALENCIA; IMAN SANTOSO; ANDI EKO MARYANTO; NIARSI MERRY HEMELDA; MARDIASTUTI H. WAHID
Biodiversitas Journal of Biological Diversity Vol. 26 No. 9 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260916

Abstract. Fadhilah QG, Elizabeht A, Falencia M, Santoso I, Maryanto AE, Hemelda NM, Wahid MH. 2025. Probiotic and genomic evaluation of Lactiplantibacillus plantarum T31 isolated from tempoyak in mung bean substrate. Biodiversitas 26: 4435-4444. Traditional fermented foods in Indonesia contain diverse beneficial microorganisms, including Lactic Acid Bacteria (LAB), which play an important role. LAB T31 has been isolated from tempoyak and evaluated for probiotic characters. In this research, the isolate was evaluated as a potential probiotic in a mung bean substrate to develop a healthy fermented drink. The result showed that LAB T31 grew well on the mung bean substrate. The population increased from 107 CFU/mL to 109 CFU/mL after 24 hours of incubation. The viability results showed that the LAB T31 population remained 10? CFU/mL after 30 days at 10°C. Cell-free filtrate of fermented mung bean substrate showed antibacterial activity against the tested bacteria. The diameter of inhibition increases from 24 hours (15-17 mm) to 48 hours (16-18 mm) of incubation. Whole genome sequences (WGS) of the isolate were performed using DNBseq technology, and the re-identification using JspeciesWS and FastANI showed that the strain belonged to Lactiplantibacillus plantarum. Analysis of the antibacterial assay test result showed that the LAB T31 genome encoded an antimicrobial peptide known as plantaricin. The hydrophobicity and auto-aggregation reached 38.86% and 36.92%, respectively, after 24 hours of incubation. Functional annotation reported adhesin genes, and the isolate did not suggest hemolytic activity on blood agar. These results supported WGS analysis using KEGG since the reported genes are not related to hemolytic ability and virulence factors. Moreover, antimicrobial resistance analysis showed that the isolate had a vanY gene, with a similarity level of 33.33%. LAB T31 was a potential isolate as a probiotic agent in the mung bean substrate.

Allelopathic activity of Dendrophthoe pentandra as a potential bioherbicide to inhibit seed germination and seedling growth of Eleusine indica Lutfan Alharits; Niarsi Merry Hemelda; Yasman; Windri Handayani
Nusantara Bioscience Vol. 12 No. 1 (2020)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n120106

Abstract. Alharits L, Hemelda NM, Yasman, Handayani W. 2020. Allelopathic activity of Dendrophthoe pentandra as a potential bioherbicide to inhibit seed germination and seedling growth of Eleusine indica. Nusantara Bioscience 12: 33-39. Dendrophthoe pentandra (L.) Miq. is a common parasitic plant in Southeast Asia. As a parasitic plant, it exhibits phytochemicals important for infecting its host, which may be potential as bioherbicide. This study was aimed to evaluate allelopathic activity of D. pentandra (stem and leaf extracts) to control Eleusine indica (L.) Gaertn. seed germination and growth. Methanolic extracts of D. pentandra stem and leaf (0, 1, 2, 3, 4 and 5 mg/mL in water) were treated on E. indica seeds in a petri dish for 10 days. After 10 days, germination of treated E. indica seeds was significantly reduced and reached >80% inhibition at 5 mg/mL. Moreover, E. indica roots were more sensitive compared to E. indica shoots, indicated by root inhibition >50% at 1 mg/mL and reached >90% inhibition at 5 mg/mL. Leaf extract presented stronger inhibition than stem extract, but not significant. It may be related to the higher accumulation of phenolic and flavonoid compounds in leaf extract than in stem extract. This is the first report of allelopathic activity in D. pentandra to inhibit E. indica germination and growth. Therefore, further study in a greenhouse, as well as metabolism effects of D. pentandra on the target weeds are important to provide comprehensive evaluation of D. pentandra potential as bioherbicide.

Title

Found 8 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 8 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 8 Documents
Search