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All Journal Biodiversitas Journal of Biological Diversity
NANIK RAHMANI
Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Cibinong Science Center. Jl. Raya Bogor Km. 46 Cibinong 16911, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Short Communication: The isolation of Klebsiella variicola’s cellulase from Macrotermes gilvus gut in Indralaya Peatlands, Indonesia DWITA OKTIARNI; HERMANSYAH HERMANSYAH; EDDY IBRAHIM; MARSI MARSI; HASANUDIN HASANUDIN; MIKSUSANTI MIKSUSANTI; DEDE HERI YULI YANTO; NANIK RAHMANI; GETARI KASMIARTI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 8 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240802

Abstract

Abstract. Oktiarni D, Hermansyah, Ibrahim E, Marsi, Hasanudin, Miksusanti, Yanto DHY, Rahmani N, Kasmiarti G. 2023. Short Communication: The isolation of Klebsiella variicola’s cellulase from Macrotermes gilvus gut in Indralaya Peatlands, Indonesia. Biodiversitas 24: 4218-4222. Termites are organisms that can inflict damage on agricultural, forest, and furniture materials. Furthermore, they can positively affect the ecosystem due to their ability to break down lignocellulose-containing materials. According to previous findings, the cellulolytic bacteria from the termite Macrotermes gilvus gut obtained in Indralaya Peatlands showed activity and hydrolyzed cellulose in a CMC agar medium. In this study, the cellulase enzymes of Klebsiella variicola isolated from Macrotermes gilvus gut showed higher cellulolytic indexes after staining with Congo red. Furthermore, the enzyme was purified and characterized using ammonium sulfate precipitation and dialysis. SDS PAGE and zymogram analysis reported a molecular mass of 20 kDa, with optimal activity at pH of 6 and temperature of 50°C.
Co-Authors DEDE HERI YULI YANTO DWITA OKTIARNI EDDY IBRAHIM GETARI KASMIARTI HASANUDIN HASANUDIN HERMANSYAH HERMANSYAH MARSI MARSI MIKSUSANTI MIKSUSANTI