OKID PARAMA ASTIRIN
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta 57126, Central Java, Indonesia

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Primer design of the CO1 gene (Cytochrome Oxidase-1) for Sumatran elephant (Elephas maximus sumatranus) for rapid detection using real-time PCR method MAQQITA TUNJUNG SARI; ARI SUSILOWATI; SETIA BETARIA ARITONANG; OKID PARAMA ASTIRIN; NITA ETIKAWATI; VIRA SAAMIA
Biodiversitas Journal of Biological Diversity Vol. 25 No. 10 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d251044

Abstract

Abstract. Sari MT, Susilowati A, Aritonang SB, Astirin OP, Etikawati N, Saamia V. 2024. Primer design of the CO1 gene (Cytochrome Oxidase-1) for Sumatran elephant (Elephas maximus sumatranus) for rapid detection using real-time PCR method. Biodiversitas 25: 3840-3849. The population of Sumatran elephants is classified as endangered according to the IUCN due to conflicts between humans and elephants, resulting in many elephants being killed by humans to obtain ivory to be sold. The traded ivory is often processed into other ornaments. A rapid forensic examination with real-time PCR using the molecular approach of the CO1 gene (Cytochrome Oxidase-1) is necessary to determine the original species of the processed elephant ivory. This research aims to design and optimize a primer for identifying Sumatran elephants (Elephas maximus sumatranus). The CO1 gene primer for Sumatran elephants was designed using the Primer3Plus and IDT websites. The primers were used to amplify and identify the species of Sumatran elephants from blood, feces, and urine samples. Blood and feces samples were collected from Sumatran elephants at Taru Jurug Zoo Surakarta, while feces and urine samples were taken from Sumatran elephants at Ragunan Zoo Jakarta. DNA from blood and urine samples was extracted using the TIANamp Genomic DNA Kit, and DNA from feces samples was extracted using the TIANamp Stool DNA Kit. The purity and concentration of the obtained DNA extracts were measured. The designed primers were synthesized by Macrogen (Korea), and primer optimization was performed with a PCR gradient. The identification process of Sumatran elephant DNA extract samples was conducted by amplification using real-time PCR quantification with the standard curve method. The results of this research include the COI-270 primer with the sequences 3'-TTAGGTCAACCAGGCTCTCTTC-5' and 5'-AGGATATACGGTCCAACCAGTG-3', capable of amplifying the target 270 bp and having a single peak at the melt curve temperature of 79°C. The identification protocol with specific COI-270 primer can be used in forensic examinations to identify samples originating from Sumatran elephants.
Assessment of biodiversity and conservation status of flora and fauna in Madiun City, East Java, Indonesia OKID PARAMA ASTIRIN; FETRIA WIDIYANTI; SURATMAN SURATMAN; NUGROHO ANDI PURNOMO; M. ARIF ROMADLON; HADI WIWIT HENDRO CAHYONO; DIAGAL WISNU PAMUNGKAS; WIDYA MEGA RAHMAWATI
Biodiversitas Journal of Biological Diversity Vol. 26 No. 8 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260826

Abstract

Abstract. Astirin OP, Widiyanti F, Suratman, Purnomo NA, Romadlon MA, Cahyono HWH, Pamungkas DW, Rahmawati WM. 2025. Assessment of biodiversity and conservation status of flora and fauna in Madiun City, East Java, Indonesia. Biodiversitas 26: 3965-3972. Madiun City in East Java, Indonesia is a small municipality that has evolved into an urban center due to its strategic position as the hub of socio-economic activities, including trade, industry, and healthcare. Urbanization in the town poses a threat to biodiversity. This is due to changes in land use and cover, natural exploitation, introduction of exotic species, air and water pollution, and climate change. This study aims to explore biodiversity in Madiun City to inform the development of strategies and policies for natural resource management. The observations revealed that Madiun City is home to 122 tree species, 76 species of ornamental plants, and 143 species of animals, including mammals, birds, reptiles, amphibians, fish, and insects. Eight plant species and five animal species are listed in the IUCN Red List. Additionally, there are eight plants and five animals listed under CITES, as well as six animals listed as protected under the Indonesian Ministry of Environment and Forests' P106/2018. Leucopsar rothschildi, Padda oryzivora, and Pavo muticus are birds listed under all the three protection instruments, suggesting their conservation importance. Green open spaces with aviaries are crucial for conserving biodiversity, as they offer a range of ecological, aesthetic, social, and economic benefits. Community participation with the local government is vital to conservation programs in urban areas. Active community involvement in conservation programs and socialization can significantly support the success of biodiversity conservation.