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EDI PURWANTO
Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta 57126, Central Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Characterization and biocontrol of Pantoea ananatis, the causal agent of rice bacterial leaf blight in Malang, Indonesia LUTFI TRI ANDRIANI; SUSILO HAMBEG POROMARTO; SUPYANI SUPYANI; EDI PURWANTO; HADIWIYONO HADIWIYONO
Biodiversitas Journal of Biological Diversity Vol. 26 No. 4 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260405

Abstract

Abstract. Andriani LT, Poromarto SH, Supyani, Purwanto E, Hadiwiyono. 2025. Characterization and biocontrol of Pantoea ananatis, the causal agent of rice bacterial leaf blight in Malang, Indonesia. Biodiversitas 26: 1537-1544. Disease outbreaks pose a significant obstacle to sustainable agricultural production. Two major diseases affecting rice crops are bacterial leaf blight and blast disease. Bacterial leaf blight caused by Pantoea ananatis is considered new, based on field observations, the cause of bacterial leaf blight is still Xanthomonas oryzae pv. oryzae. Research on P. ananatis is important to provide new knowledge about the causes of bacterial leaf blight other than X. oryzae pv. oryzae. The symptoms of bacterial leaf blight include yellowing and browning of the leaf, followed by drying, resulting in death of the leaf cells. The objectives of this study were to isolate the bacteria, identify them morphologically and molecularly, determine physiological characteristics and explore their biocontrol potential using rhizobacteria. Bacterial characterization was performed both morphologically and physiologically. This study determined the hypersensitive response of tobacco leaves, physiological and biochemical characteristics, pathogenicity tests, and molecular identification via 16S rRNA gene sequencing. The bacteria formed yellow colonies on yeast chalk dextrose agar at 36°C. Isolate was positive for indole and Levan tests, used citrate as a carbon source, and liquefied gelatin at low temperatures. The bacteria showed positive results in tobacco hypersensitivity and pathogenicity tests on paddy rice. Molecular identification results showed that the bacterial pathogen was identified as P. ananatis. The result of biocontrol potential test was shown that bacteria with the code UNS-R2 can control the pathogenic bacteria P. ananatis using spray method. These findings can be used as new knowledge about the types of leaf blight pathogens other than X. oryzae and their control alternatives to determine tactical steps in integrated crop management toward sustainable agriculture. This is the first report on the identification and characterization of P. ananatis in rice in Indonesia and the biocontrol potential using rhizobacteria.
Secondary metabolic analysis of three shallot cultivars on coastal peatlands in Meranti Islands District, Riau Province, Indonesia SELVIA SUTRIANA; SAMANHUDI SAMANHUDI; EDI PURWANTO; HASAN BASRI JUMIN
Biodiversitas Journal of Biological Diversity Vol. 26 No. 5 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260509

Abstract

Abstract. Sutriana S, Samanhudi, Purwanto E, Jumin HB. 2025. Secondary metabolic analysis of three shallot cultivars on coastal peatlands in Meranti Islands District, Riau Province, Indonesia. Biodiversitas 26: 2106-2116. Shallot (Allium ascalonicum) is a strategic horticultural vegetable commodity with great potential to be developed due to the various secondary metabolite content, such as flavonoid, phenol, anthocyanin, and antioxidant activity, which are crucial for health. This study aimed to identify and determine the secondary metabolism values of three shallot cultivars in coastal peatlands, namely Bima Brebes, Trisula, and SS Sakato. Shallot bulbs were extracted by maceration, followed by phytochemical content filtration. The total phenol, anthocyanin, as well as flavonoid levels were assessed using High-Performance Liquid Chromatography (HPLC), while antioxidant activity was estimated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results indicated that SS Sakato cultivar had the highest extract yield of 12.17%. Bima Brebes and Trisula cultivars contained flavonoid, phenol, terpenoid, and steroid phytochemical, while SS Sakato had flavonoid, phenol, and terpenoid. Moreover, Bima Brebes produced the highest total phenol and flavonoid and SS Sakato had the highest anthocyanin. The standard retention time of quercetin from three shallot cultivars ranged from 9.167 to 9.227 minutes, with an average of 9.192 minutes. Bima Brebes was also found to have the best antioxidant activity as indicated by Inhibition Concentration50 (IC50) value.
Genetic characterization of butterfly pea (Clitoria ternatea) with different flower colors and petal types using SRAP markers SUSANTI INDRIYA WATI; SAMANHUDI SAMANHUDI; ENDANG YUNIASTUTI; EDI PURWANTO
Biodiversitas Journal of Biological Diversity Vol. 26 No. 8 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260806

Abstract

Abstract. Wati SI, Samanhudi S, Yuniastuti E, Purwanto E. 2025. Genetic characterization of butterfly pea (Clitoria ternatea) with different flower colors and petal types using SRAP markers. Biodiversitas 26: 3722-3730. The butterfly pea (Clitoria ternatea) is an essential medicinal and ornamental plant in Indonesia, known for its diverse petal colors and forms. This study aimed to assess genetic diversity among eight C. ternatea accessions with varying flower colors and petal types using 10 SRAP primer combinations. The 114 DNA bands were amplified, and the average degree of polymorphism across all primer combinations was 87.12%. Two primer combinations (Me3Em3 and Me4Em4) displayed 100% polymorphism. The markers showed an average Polymorphic Information Content (PIC) of 0.31, while the mean Resolving Power (RP) and Marker Index (MI) were 5.50 and 3.24, respectively. The genetic relationships using UPGMA and PCoA revealed two main groups: Cluster 1, which included 1U (purple flower, single petal), 1Pn (pink flower, single petal), 2U (purple flower, double petal), and 2Pn (pink flower, double petal), and Cluster 2, which included 1B (blue flower, single petal), 1Pt (white flower, single petal), 2B (blue flower, double petal), and 2Pt (white flower, double petal). STRUCTURE analysis revealed a more precise genetic separation, with accessions 1U, 1B, 1Pn, and 1Pt clustered in Cluster 1, and 2U, 2B, 2Pn, and 2Pt clustered in Cluster 2. These findings are not only important for breeding programs, but also for conservation strategies. They provide valuable insights that can guide efforts to preserve the genetic diversity of C. ternatea, highlighting the crucial role of this research in the broader context of plant conservation.
Responses of black soybean to rice husk-derived bio-silica nanoparticles and mycorrhiza on physiology and root development under drought stress DWI SUCI LESTARIANA; EDI PURWANTO; MUJI RAHAYU; SUPRIYONO SUPRIYONO
Biodiversitas Journal of Biological Diversity Vol. 26 No. 11 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261139

Abstract

Abstract. Lestariana DS, Purwanto E, Rahayu M, Supriyono. 2025. Responses of black soybean to rice husk-derived bio-silica nanoparticles and mycorrhiza on physiology and root development under drought stress. Biodiversitas 26: 5824-5837. This study investigated the combined effects of rice husk-derived bio-silica nanoparticles and Arbuscular Mycorrhizal Fungi (AMF) on the physiological and root development responses of two contrasting black soybean (Glycine max) cultivars, Malika and Detam 2. A factorial experiment was arranged in a completely randomized design with three replicates. Treatment included two cultivars (Malika, Detam 2), AMF (inoculated, non-inoculated), and five biosilica nanoparticle concentrations (0, 50, 100, 150, 200 ppm). Data were analyzed using ANOVA, and mean differences were compared with Duncan's Multiple Range Test (DMRT). Results showed that cultivar Malika maintained 0.72% (p<0.05) lower H?O? accumulation and stronger antioxidant activity, while cultivar Detam 2 exhibited better root development when treated with 150-200 ppm biosilica nanoparticle and combined with AMF inoculation, showing the longest length (37 cm; p<0.05) and the greatest volume (3.17 cm³; p<0.05). These improvements indicate that bio silica nanoparticles supported redox stability and osmotic regulation, while AMF strengthened nutrient acquisition and root elongation. Overall, the integrated application of AMF and bio silica nanoparticles induced complementary biochemical and morphological tolerance mechanisms in both cultivars, enhancing drought resilience through reduced oxidative stress, improved osmotic balance, and strengthened root development. This study provides evidence of synergistic interactions between AMF and rice husk-derived bio-silica nanoparticles in black soybean, offering a sustainable, waste-based strategy to improve legume productivity under dryland and climate-stress conditions.
Co-Authors . Hadiwiyono DWI SUCI LESTARIANA ENDANG YUNIASTUTI HASAN BASRI JUMIN LUTFI TRI ANDRIANI MUJI RAHAYU SAMANHUDI SAMANHUDI SELVIA SUTRIANA SUPRIYONO SUPRIYONO SUPYANI SUPYANI SUSANTI INDRIYA WATI SUSILO HAMBEG POROMARTO