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All Journal Canrea Journal: Food Technology, Nutritions, and Culinary Journal Indonesian Journal of Life Sciences Molekul: Jurnal Ilmiah Kimia

Agus Budiawan Naro Putra

Indonesia International Institute For Life Sciences

Author-ID : 1098774

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology Medicine & Pharmacology

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Articles

ANTI-ALLERGY POTENTIAL OF PETIS EXTRACT ON IMMUNOGLOBULIN E PRODUCTION BY U266 CELLS: (Potensi Anti-alergi Ekstrak Petis terhadap Produksi Imunoglobulin E oleh Sel U266) Rosalind Vivia Tansy; Agus Budiawan Naro Putra; Takuya Sugahara
jurnal1 VOLUME 1 ISSUE 2, DESEMBER 2018
Publisher : Hasanuddin University Food Science and Technology Study Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/canrea.v1i2.99

Petis, one of Indonesia’s traditional condiment, is most often made from shrimp andshrimp waste. This condiment becomes popular as a savory flavor additive which is mainly produced in East Java, Indonesia. Despite the unique taste and good nutritionalcontent, any particular study regarding its health benefit in Indonesia has not thoroughly evaluated yet. Therefore, the objective of this research was to explore petis potential towards anti-allergy property in vitro. The potential property was evaluated by determining the concentration of Immunoglobulin E (IgE) by U266 cells treated with petis extract using ELISA. In result, petis extract could significantly suppressed IgE production up to 2-fold atits highest concentration compared to control. Further investigation to extrapolate petis functional bioactive compound was conducted by treating petis extract with heat and enzyme (proteinase K). Result showed that heat-and enzyme-treated petis extract still have the ability to suppress IgE production by U266 cells. Thus,it could be assumed that the functional bioactive compound was a heat-stable non-protein compound. Thesepreliminary findings could conclude that petis extract has a potential anti-allergy propertywhich gives an added value towards petis product in Indonesia.

Water and ethanol extracts of tamarind (Tamarindus indica) suppress lipid accumulation in 3T3-L1 cells Kenn Rafael Harjadi; Momoko Ishida; Kosuke Nishi; Takuya Sugahara; Agus Budiawan Naro Putra
jurnal1 VOLUME 5 ISSUE 1, JUNE 2022
Publisher : Hasanuddin University Food Science and Technology Study Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/canrea.v5i1.630

Obesity is one of the major health problems which could trigger the development of many other non-communicable diseases. Tamarind (Tamarindus indica) is a multi-purpose plant that provides many health benefits, including anti-obesity potentials. In Indonesia, it is often used as a traditional food component in many dishes. A recent in vivo study proved that tamarind might reduce the bodyweight of obese rats. To further investigate the anti-obesity effect of tamarind, an in vitro study using 3T3-L1 pre-adipocytes was conducted. During differentiation, 3T3-L1 cells synthesize and accumulate lipid in the form of triglycerides in the cytoplasm. Tamarind water and ethanol extracts were incorporated into the medium during the differentiation stage of the cells. Oil red O staining assay was used to measure the total lipid accumulated. Results showed that both tamarind water and ethanol extracts exhibited non-cytotoxicity effects on 3T3-L1 cells. In addition, tamarind water extract at 10 mg/mL decreased the total lipid accumulated significantly to 80.5% compared with that of control, while tamarind ethanol extract showed less lipid accumulation inhibitory effect at any doses tested. This study revealed that tamarind water extract has the potential to reduce lipid accumulation, thus showing an anti-obesity effect.

Immunostimulatory effect of ikan peda (fermented Rastrelliger sp.) water extract on IgM production by HB4C5 cells Anindita, Michaela Maria Kanya; Putra, Agus Budiawan Naro; Sugahara, Takuya
Indonesian Journal of Life Sciences 2019: IJLS Vol 01 No .01
Publisher : Indonesia International Institute for Life Sciences

Ikan peda is one of Indonesia’s traditional fermented food products. It uses mackerel fish (Rastrelliger sp.), also known as ikan kembung in Indonesia, as its main ingredient. It is made through fermentation by adding high concentration of salt in order to extend the shelf-life and bring out the desired flavor of the fish. Although fermented foods are known to be beneficial towards health, fewer studies have been reported regarding the actual health promoting potential of Indonesian fermented foods, especially ikan peda. Thus, the immunostimulatory effects of ikan peda extract were examined. Water-extract of ikan peda was tested to human-human hybridoma HB4C5 cells. It was found that ikan peda extract stimulates the production of IgM by HB4C5 cells according to ELISA results. Data from real-time RT-PCR result showed that ikan peda extract has the ability to increase IgM gene expression by 3-fold at the highest concentration treatment. Results from the heat and enzyme treatments showed that the active substance contained in the ikan peda extract was expected to be a heat-stable protein. Although, the active substance and the mechanism is yet to be determined, and can be considered for future experiments. Our findings suggest that consumption of ikan peda would contribute to the promotion of health.

Botanical Aspects, Phytochemicals and Health Benefits of Andaliman (Zanthoxylum acanthopodium) Natasutedja, Alfredo Oktavianto; Lumbantobing, Erika; Josephine, Emita; Carol, Lioni; Junaedi, Decky Indrawan; Normasiwi, Suluh; Putra, Agus Budiawan Naro
Indonesian Journal of Life Sciences 2020: IJLS Vol 02 No .01
Publisher : Indonesia International Institute for Life Sciences

Andaliman (Zanthoxylum acanthopodium) is a specialty spice widely found in the Provinces of North Sumatra and Aceh, Indonesia. The fruit is usually used by Batak people in North Sumatra as a food ingredient to cook Batak’s cuisine such as arsik, tombur, and naniura. It is also used as one of the ingredients to make Indonesian spicy sauce called sambal. Andaliman fruit is reported to eliminate the undesirable smell in fish and raw meat. Moreover, it could also reduce total volatile base nitrogen content in fish which helps to prevent spoilage. Furthermore, andaliman has been reported to contain bioactive compounds which act as antioxidant, antimicrobial, anti-inflammatory, anti-aging, anti-acne, and anti-halitosis effects. This review focuses on the botanical aspects of and health potential exhibited by andaliman.

Inulin from Several Tubers Available in Indonesia and the Growth of Gut Microbiota Crespo, Ivan; Arindra, Augusto; Lualdi, Jessica Tashia; Rimba, Phoebe Anadita; Putra, Agus Budiawan Naro; Rahardja, Ritchie
Indonesian Journal of Life Sciences 2020: IJLS Vol 02 No .01
Publisher : Indonesia International Institute for Life Sciences

Indonesia has many types of tubers, some of them are gembolo tuber (Dioscorea bulbifera), lesser yam (Dioscorea esculenta), yam bean (Pachyrhizus erosus), and dahlia tuber (Dahlia pinnata); which are the sources of prebiotics. The term of prebiotics has been used since 1995 to describe the non-digestible components in food that positively alter gut microbiota activity and exhibit beneficial properties to the human body. One of the prebiotics that can be found in tubers is inulin. This review was aimed to compare inulin content in the above-mentioned tubers and their roles towards the growth of gut microbiota. Library searches using Semantic Scholar database was done. Findings showed that gembolo tuber contains the highest inulin content, followed by lesser yam, dahlia tuber, and yam bean. Moreover, inulin increases the population of Lactobacillus, Bifidobacterium, and Bacteroides while inhibits the growth of Escherichia coli and reduce the growth of Clostridium.

Colletotrichum sp. from Nutmeg Leaves Shows Antiproliferative Activity Against MCF-7 Cancer Cells: In Vitro Evaluation, Metabolite Profiling, and Docking Analysis Aisyah, Siti; Suryati, Suryati; Putra, Agus Budiawan Naro; Palupi, Kartika Dyah; Rahmi, Dwinna; Ilyas, Muhammad; Agusta, Andria
Molekul Vol 20 No 3 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.3.16748

ABSTRACT. Endophytic fungi are recognized as a valuable source of bioactive compounds with significant therapeutic potential, with species from the genus Colletotrichum being particularly noted for their prolific production of structurally diverse secondary metabolites. This study aimed to evaluate the antiproliferative activity of the endophytic fungus Colletotrichum sp., isolated from the leaves of Myristica fragrans Houtt., commonly known as nutmeg against MCF-7 human breast cancer cells using an integrated in vitro and in silico approach. Bioassay-guided fractionation of the fungal extract followed by LC-HRMS analysis led to the identification of three major compounds: 2-amino-1,3,4-octadecanetriol, L-α-palmitin, and muscone-like compound. The antiproliferative activity of each subfraction was assessed using the MTT assay, while molecular docking studies were conducted to evaluate their binding affinities and interaction profiles with estrogen receptor α (ERα). Among the tested subfractions, F2.3 demonstrated the strongest antiproliferative activity, reducing MCF-7 cell viability to 22.08% at a concentration of 50 µg mL-1. The identified compounds demonstrated notable antiproliferative potential, with L-α-Palmitin showing the moderate binding affinity to ERα as indicated by a binding energy of -6.406 kcal mol-1. Molecular docking analyses revealed key interactions, including hydrogen bonding and hydrophobic contacts, contributing to receptor-ligand stability. These findings highlight Colletotrichum sp. as a promising source of antiproliferative agents, thus warranting further investigation into the isolation, structural characterization, and in vivo validation of its active constituents for potential pharmaceutical development. Keywords: antiproliferative, Colletotrichum sp., endophytic fungi, LC-HRMS, Myristica fragrans Houtt., secondary metabolites.

Co-Authors ANDRIA AGUSTA Anindita, Michaela Maria Kanya Arindra, Augusto Carol, Lioni Crespo, Ivan Decky Indrawan Junaedi Dwinna Rahmi Josephine, Emita Kartika Dyah Palupi Kenn Rafael Harjadi Kosuke Nishi Lualdi, Jessica Tashia Lumbantobing, Erika Momoko Ishida Muhammad Ilyas Natasutedja, Alfredo Oktavianto Rahardja, Ritchie Rimba, Phoebe Anadita Rosalind Vivia Tansy Siti Aisyah Sugahara, Takuya suluh normasiwi, suluh Suryati Suryati Takuya Sugahara Takuya Sugahara

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