Danang Kurniawan, Tri
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ANALISIS PENYALAHGUNAAN RHODAMIN B PADA LIPTINT YANG BEREDAR DI KOTA BENGKULU MENGGUNAKAN METODE KROMATOGRAFI LAPIS TIPIS DAN SPEKTROFOTOMETRI UV-VIS Ramadhani, Oktalia; Mulia, Putri; Danang Kurniawan, Tri; Intan Perma Sari , Rose; Kurnia Putri, Dwi
Pendas : Jurnal Ilmiah Pendidikan Dasar Vol. 11 No. 02 (2026): Volume 11 No. 2, Juni 2026 Publish
Publisher : Program Studi Pendidikan Guru Sekolah Dasar FKIP Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/jp.v11i02.51357

Abstract

Liptint is a popular cosmetic product because it gives lips a natural color. However, some products still contain hazardous substances such as Rhodamine B, which is toxic and carcinogenic. This study aimed to detect the presence of and measure the levels of Rhodamine B in lip tints sold in several markets in Bengkulu City. A total of 15 samples were analyzed. Qualitative analysis was performed using Thin-Layer Chromatography (TLC), while quantitative analysis utilized UV-Vis Spectrophotometry. TLC test results indicated that two samples (PM3 and PK3) tested positive for Rhodamine B, as evidenced by Rf values close to the standard and fluorescence under UV light. Meanwhile, the results of quantitative analysis at a maximum wavelength of 542.95 nm showed Rhodamine B levels of 0.0332% in sample PM3 and 0.0129% in sample PK3. Based on the results obtained, it can be concluded that lip tints testing positive for Rhodamine B are not permitted for distribution in the market in accordance with the Regulation of the Head of the Food and Drug Supervisory Agency, and that Rhodamine B has highly dangerous effects if used over the long term.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN DADAP SEREP (ERYTHRINA LITHOSPERMA MIQ) TERHADAP PERTUMBUHAN BAKTERI SALMONELLA TYPHII ATCC 13313 putri wardani, Erlin; Sipriyadi; mulia, Putri; Slamet, Samwilson; danang kurniawan, Tri
Pendas : Jurnal Ilmiah Pendidikan Dasar Vol. 11 No. 02 (2026): Volume 11 Nomor 02, Juni 2026 Published
Publisher : Program Studi Pendidikan Guru Sekolah Dasar FKIP Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/jp.v11i02.52963

Abstract

The bacterium Salmonella typhii ATCC 13313 causes typhoid fever, which remains a medical problem in Indonesia. To prevent bacterial resistance, the misuse of antibiotics may lead to the use of alternative antibacterial agents derived from natural sources. The dadap serep leaf, or Erythrina lithosperma Miq, is a plant with potential as an antibacterial agent because it contains secondary metabolites such as alkaloids, flavonoids, triterpenoids, and phenols. The objective of this study was to determine the antibacterial properties of a 96% ethanol extract from dadap serep leaves that affect the growth of Salmonella typhii ATCC 13313. The disk diffusion method was used in this study. The maceration method with 96% ethanol was used to prepare the dadap serep leaf extract. Concentrations of 20%, 40%, 60%, 80%, and 100% were tested. Chloramphenicol was used as a positive control, and DMSO was used as a negative control. After 24-hour incubation at 37°C, inhibition zones around the disk paper indicated antibacterial activity. The results of the study show that the ethanol extract of Erythrina lithosperma Miq. leaves has a yield of 13.86% and is capable of inhibiting the growth of Salmonella typhii ATCC 13313. The higher the concentration of the extract, the greater the inhibitory effect it produces. Therefore, the ethanol extract of Erythrina lithosperma Miq. leaves may serve as a natural antibacterial agent against Salmonella typhii ATCC 13313.
FORMULASI DAN EVALUASI EMULGEL EKSTRAK ETANOL 96% DAUN CENGKEH (Syzygium aromaticum) DENGAN VARIASI KONSENTRASI EKSTRAK Sasi Syabrina, Cinta; Intan Perma Sari, Rose; Danang Kurniawan, Tri; Mulia, Putri; Ikhsan
Pendas : Jurnal Ilmiah Pendidikan Dasar Vol. 11 No. 02 (2026): Volume 11 Nomor 02, Juni 2026 Published
Publisher : Program Studi Pendidikan Guru Sekolah Dasar FKIP Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/jp.v11i02.53582

Abstract

Clove leaves (Syzygium aromaticum) contain flavonoids and eugenol, which have the potential to act as natural antioxidants to protect the skin from free radical damage. This study aimed to formulate an ethanolic extract of clove leaves into a stable emulgel and determine the extract concentration that produces the best physical characteristics. The emulgel was chosen because it combines the advantages of an emulsion in delivering hydrophobic compounds with the comfort of gel application on the skin.The laboratory experimental study began with the preparation of clove leaf crude extract and extraction using a maceration method with 96% ethanol as a solvent. The resulting extracts were formulated into four formulas: F0 (base without extract), F1 (4%), F2 (8%), and F3 (12%), using Carbopol 940 as a gelling agent. Physical quality evaluation included organoleptic tests, homogeneity, pH, spreadability, viscosity, emulsion type, and hedonic tests.The results showed that all formulas were homogeneous and oil-in-water (O/W) emulsions. The pH value ranges from 5.00 to 5.93, matching the skin's pH. Spreadability ranges from 5.14 to 5.97 cm, and viscosity ranges from 5599.67 to 9080.33 cPs, meeting the requirements for topical formulations. Increasing the extract concentration affects the color, aroma, and reduces the viscosity of the formulation. Formulas F2 and F3 demonstrated the most optimal physical characteristics and spreadability.