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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Zuriat
Manalu, Victor Manotar Pademan
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Education

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Seleksi Marka SSR untuk Toleransi Terhadap Cekaman Suhu Tinggi pada Populasi F2 Padi Manalu, Victor Manotar Pademan; Wirnas, Desta; Sudarsono, Sudarsono
Zuriat Vol 34, No 2 (2023): September, 2023
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v34i2.49422

Abstract

Penelitian ini bertujuan untuk memperoleh primer SSR terpaut toleran terhadap suhu tinggi dengan menggunakan bulked segregant analysis (BSA) dan dilanjutkan dengan single marker analysis (SMA). Penelitian dilakukan di Laboratorium Biologi Molekuler Tanaman, Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor (IPB). Bahan genetik yang digunakan DNA tetua IPB 4S, Situ Patenggang, DNA dari genotipe F2, 12 Primer SSR. Hasil penelitian menunjukkan primer SSR RM 337 mengikuti segregasi hukum Mendel, kemudian berdasarkan  single marker analysis dengan menggunakan karakter bobot gabah bernas menunjukkan primer SSR RM 337 terpaut toleran terhadap suhu tinggi dengan nilai peluang yang sangat nyata. Genotipe F2 yang memiliki pola pita DNA seperti Situ Patenggang (Tetua toleran) dan daya hasil tinggi menghasilkan 54 genotipe F2. Diferensial seleksi berdasarkan genotyping dengan menggunakan primer RM 337 menghasilkan kenaikan bobot gabah bernas sebesar 37.96%.
Karakter Seleksi pada Generasi Awal untuk Adaptasi Padi terhadap Cekaman Suhu Tinggi Manalu, Victor Manotar Pademan; Wirnas, Desta; Sudarsono, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 2 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.896 KB) | DOI: 10.24831/jai.v45i2.12938

Abstract

ABSTRACT            Developing new rice  varieties adaptive to heat stress is important to maintain high rice production in anticipating global warming effects. This research was aimed to find selection characters and to select the best segregant in early generation based on agronomic characters for adaptation of rice to heat stress. The experiment was carried out in September 2014 to Januari 2015. About 210 F2 segregants generated trough hybridization between IPB 4S (sensitive parent) and Situ Patenggang (tolerant parent), 20 individuals of IPB 4S, and 20 individuals of Situ Patenggang, were used as genetic control. All genetic materials were exposed to high temperature stress by growing in a green house of Bogor Agricultural University. The average temperature in the research period was 24 °C and 42.09 °C, respectively for minimum and maximum temperature.  The results showed that charachters of productive and total tiller number, total grain number, and seed weight had high heritability, high coeffiecient of genetics variability-with an additive gene action. That charachters could be proposed as selection criteria in early generation for rice breeding of adaptation to high temperature stress. The characters could be applied  in either single trait or multiple traits selection. Based on multiple traits selection we found 50 the best F2 segregation.Keywords: differential selection, early generation, heat tolerant, heritability, gene action, variability genetics coeffiecient
Co-Authors Desta Wirnas Sudarsono Sudarsono, dan