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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Silvikultur Tropika (JST) Agrotrop : Journal on Agriculture Science Bionatura Zuriat Jurnal Natur Indonesia Atom Indonesia Journal Jurnal Ilmiah Aplikasi Isotop Radiasi Jurnal Agro Teknologi Tropika Jurnal Ilmu Komputer dan Agri-Informatika Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Pelita Perkebunan Jurnal Agroekoteknologi Jurnal Agro Jurnal Penelitian Karet Tropical Animal Science Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Journal of Tropical Crop Science JURNAL BUDIDAYA PERTANIAN Jurnal Agrotek Lestari Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Warta Perkaretan Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Natural

Desta Wirnas

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia

Author-ID : 251701

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Neuroscience Physics Social Sciences Veterinary

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ANALISIS DAYA HASIL LATEKS DAN HERITABILITAS KARAKTER KUANTITATIF DARI BEBERAPA GENOTIPE KARET PP/07/04 Sayurandi, Sayurandi; Wirnas, Desta; Woelan, Sekar
Jurnal Penelitian Karet JPK : Volume 34, Nomor 1, Tahun 2016
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v34i1.226

Tujuan penelitian ini adalah untuk mempelajari karakter pertumbuhan, daya hasil lateks dan heritabilitas karakter kuantitatifÂ beberapa genotipe karetÂ PP/07/04. Sebanyak lima belasÂ genotipe karet dan dua klon pembanding PB 260 dan RRIC 100 diuji pada penelitian ini. Pengujian genotipe tersebut dibangun di Kebun Percobaan Balai Penelitian Sungei Putih, Pusat Penelitian Karet pada tahun 2004, yang terletak di Kabupaten Deli Serdang - Provinsi Sumatera Utara. Rancangan yang digunakan pada penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan tiga ulangan. Hasil penelitian menunjukkan bahwa genotipe berpengaruh nyata terhadap dua belas karakter yang diamati yaitu lilit batang, tebal kulit, jumlah cincin pembuluh lateks, diameter sel pembuluh lateks, panjang alur sadap, kecepatan aliran lateks, indeks penyumbatan, kadar fosfat anorganik, kadar sukrosa, kadar thiol, kadar karet kering, danÂ daya hasil lateks. Berdasarkan karakter pertumbuhan dan daya hasil lateks menunjukkan bahwa genotipe HP 92/309 memiliki potensi hasil lateks tinggi, sedangkan genotipe HP 92/542 memiliki hasil lateks tinggi dan pertumbuhan jagur. Dua belas karakter yang diamati memiliki nilai heritabilitas tinggi dengan nilai h2bs antara 0,52 â 0,95. Hal ini menunjukkan bahwa karakter-karakter tersebut lebih dipengaruhi oleh faktor genetik.Â Diterima : 5 April 2016 / Direvisi : 30 Juni 2016 / Disetujui : 3 Agustus 2016Â How to Cite : Sayurandi, S., Wirnas, D., & Woelan, S. (2016). Analisis daya hasil lateks dan heritabilitas karakter kuantitatif dari beberapa genotipe karet PP/07/04. Jurnal Penelitian Karet, 34(1), 1-12. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/226

Pewarisan Karakter Toleransi Aluminium Tanaman Sorgum Manis [Sorghum bicolor (L.) Moench] pada Stadia Bibit Isnaini, Isnaini; Trikoesoemaningtyas, Trikoesoemaningtyas; Wirnas, Desta
Jurnal Agro Teknologi Tropika Vol 3, No 2 (2014)
Publisher : Jurnal Agro Teknologi Tropika

Show Abstract | Download Original | Original Source | Check in Google Scholar

The objective of this research was to study the inheritance of Al tolerance of sweet sorghum at seedling stage. Thefour populations in this research were UPCA S1 (P1, susceptible parent) and Numbu (P2, tolerant parent), F1 and F1reciprocal and F2. The crosses of parental, F1, F1R and F2 generation were grown in University Farm of IPB and UPTDTenjo from June 2008 to June 2009. The study of Al tolerance inheritance in nutrient solution was conducted in greenhouse of University Farm of IPB from July-August 2009. Based on reciprocal analysis, all of characters are controlled bynuclear genes and their inheritance in not affected by maternal cytoplasm. The distribution of F2 genotype indicatedcontinuous with skewnesses which indicate that all characters are polygenics. All characters of Al tolerance in seedlingstage i.e. root length, root and shoot dry weight were controlled by additive gene action with contribution bycomplementary epistasis gene except shoot length that is controlled by additive gene action with duplicate epistasisgene. All characters had broad sense heritability from medium to high. Based on heritability value, pedigree selectionis the most effective selection method in breeding program for sorghum tolerance to Al.

Penggunaan Mikro Irradiasi Sinar Gamma untuk Meningkatkan Keragaman Genetik pada Varietas Kedelai Argomulyo [Glycine max (L) Merr] Hanafiah, Diana Sofia; Trikoesoemaningtyas, Trikoesoemaningtyas; Yahya, Sudirman; Wirnas, Desta
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

This research used gamma ray irradiation on low doses (micro mutation). The aim of this research was to know the respons of doses level bymicro mutation on gamma ray irridation to the growing and development of Argomulyo variety of Glycine max (L) Merr (M1), the seeds isirradiated by gamma ray by micro mutation was 0 Gy, 50 Gy, 100 Gy, 150 Gy, and 200 Gy. Variation that obtained of each characters atgeneration M1 and M2 influences plants growth and development either through qualitative and quantitative that finally will influence plantsproduction. The average highest genetic variation at M 2 generation of soybean was on 200 Gy doses. Results of the research indicated thatgamma ray irradiation on 200 Gy doses was effective caused of plant variation genetic.

Dampak Suhu Tinggi terhadap Pertumbuhan dan Hasil Tanaman Padi Usamah Jaisyurahman; Desta Wirnas; Trikoesoemaningtyas; Heni Purnamawati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 3 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Global warming becomes a pressure in food production sustainability because it affected crop growth and development. The purpose of this study was to obtain information on the effect of high-temperature stress on the growth and development phase of rice and to evaluate the genotype for tolerance to high-temperature stress. Two environment conditions were used in the field and greenhouse of IPB Cikabayan experimental field, IPB University from August 2016 until February 2017. The study used varieties of IPB 4S, IPB 6R, Mekongga, and Situ Patenggang. High-temperature treatment was done by transferring the rice plants to the greenhouse at 50 days after transplanting. Observations were made on the generative phase in two different environmental conditions. The results showed that the total tillers number, filled grain number per panicle, unfilled grain number per panicle, total grain number per panicle, grain filling rate, percentage of filled grain and filled grain weight per plant had different responses among rice genotypes due to high-temperature stress. High-temperature decreased pollen fertility in all genotypes, which classified IPB 4S as a sensitive genotype and Mekongga as a tolerant genotype. This information could be useful for development and improving rice variety to anticipate high-temperature stress. Keywords: Climate change, fertility, pollen, stress tolerance index

Efektivitas Metode Seleksi Pedigree dan Modified Bulk pada Tiga Populasi Sorgum (Sorghum bicolor [L.] Moench) Dewi Andriani; Desta Wirnas; Trikoesoemaningtyas
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 3 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Sorghum has a growing popularity for food, feed, biofuel, and therefore needs to be developed. The research aimed to compare the effectiveness of pedigree and modified bulk selection methods for improving yield in three sorghum populations. The genetic materials used were 60 F6 lines from three populations, namely PI 10-90-A x Numbu, PI 150-20-A x Numbu and PI 150-20-A x Kawali which had been selected using pedigree and modified bulk methods. The experiment was conducted from October 2018 to February 2019 at the Cikabayan Experimental Station of IPB University, Dramaga, Bogor. The experiment was arranged in an augmented design with six checks, namely Kawali, Numbu, PI 10-90-A, PI 150-20-A, Samurai 1, and Samurai 2 which were replicated four times. Observations were made on agronomic traits and yield. Plant height and grain weight per panicle had high heritability and large genotypic coefficients of variation. The contrast test results showed significant differences between populations in plant height, panicle length, days of harvesting and 100-seed weight. On the other hand, no significant difference was observed among selection methods, indicating that both methods were equally effective for increasing sorghum yield. The pedigree selection and modified bulk selection increased grain yield per panicle by 14.1 g and 18.2 g respectively. Bulk of the best genotypes in early generation could be an alternative of bulk selection method. Keywords: contrast test, differential selection, genotypic coefficient of variation, heritability

Estimation of Genetic Components of Cocoa Populations (Theobroma cacao L.) in Seedling Phase Agung Wahyu Susilo; Sobir; Desta Wirnas; Wuriandani, Adinda
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 48 No. 1 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Penggunaan bibit sebagai bahan tanam maupun batang bawah untuk perbanyakan klonal sering dilakukan. Bahan tanam berupa bibit merupakan bahan tanam unggul yang berasal dari tetua unggul maupun persilangan antar tetua unggul. Penelitian ini bertujuan untuk mengetahui nilai duga komponen genetik melalui desain persilangan North Carolina II pada karakter morfologi tanaman kakao fase bibit. Persilangan dilakukan dengan menggunakan lima genotipe tetua betina, yaitu; KW 516, Sulawesi 3, TSH 858 dan dua genotipe tetua jantan, yaitu; ICCRI 09, Scavina 6. Pengamatan dilakukan pada tanaman umur 16 MST pada karakter morfologi tanaman yaitu tinggi tanaman, diameter batang, jumlah daun, panjang akar, volume akar, bobot basah tajuk, bobot basah akar, bobot kering tajuk, bobot kering akar, dan rasio akar/tajuk. Genotipe Sulawesi 3 merupakan genotipe tetua betina terbaik, sedangkan Scavina 6 merupakan genotipe tetua jantan terbaik. Genotipe persilangan terbaik berdasarkan nilai DGK nya adalah genotipe TSH 858 x Scavina 6, KW 516 x ICCRI 09, dan Sulawesi 3 x ICCRI 09. Genotipe persilangan Sulawesi 3 x ICCRI 09 merupakan genotipe dengan vigor yang baik yang memenuhi kriteria bibit unggul dan siap untuk ditanam di kebun. Karakter morfologi pada tanaman kakao fase bibit dikendalikan oleh gen dominan pada semua karakter kecuali pada karakter volume akar. Kata kunci: bibit, daya gabung khusus, daya gabung umum, North Carolina II

Molecular Analysis and Phenotypic Performances of BC3F2 Upland Rice Lines Containing Alt and Pup1 Loci Mizan, Muhammad Rauful; Wirnas, Desta; Tasliah, nFN; Hidayatun, Nurul; Prasetiyono, Joko
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p83-92

The challenges in upland rice cultivation are phosphorous (P) deficiency and aluminum (Al) toxicity, each controlled by Pup1 and Alt loci, respectively. Pyramiding the two genes into Indonesian rice varieties were previously done through Marker-Assisted Backcrossing method to obtain BC3F2 populations. The aims of this study were to analyze the BC3F2 upland rice lines containing the Alt and Pup1 loci molecularly (foreground and background analyses) and to test their phenotypic performances. Genetic materials tested included Dupa variety (donor of Alt) and three improved Indonesian genotypes (Dodokan-Pup1, Situ Bagendit-Pup1, and Batur-Pup1) as recurrent parents, Kasalath (donor of Pup1), and 300 BC3F2 lines from Dodokan-Pup1+Alt, Situ Bagendit-Pup1+Alt, and Batur-Pup1+Alt, respectively. The rice genotypes were selected individually using modified Yoshida nutrient solution, followed by foreground and background analyses. 150 out of 300 seedlings were selected and maintained until harvest in the greenhouse. Foreground analysis using markers (RM1361, RM12031, and Kas46-2) and tiller number performances resulted in 18 plants from BC3F2 Dodokan-Pup1+Alt, 30 plants from BC3F2 Situ Bagendit-Pup1+Alt, and 25 plants from BC3F2 Batur-Pup1+Alt still carrying Alt and Pup1 loci. Background analysis using molecular markers showed that the best individual lines of BC3F2 were number 56 for BC3F2 Dodokan-Pup1+Alt, number 32 or 70 for BC3F2 Situ Bagendit-Pup1+Alt, and number 20 for BC3F2 Batur-Pup1+Alt. The selected lines having both both Alt and Pup1 loci in homozygote condition with highest number of tiller per plant which are useful genetic materials for developing upland rice variety tolerance to low P and Al toxicity.

Characterization of cDNA for PMT: a Partial Nicotine Biosynthesis-Related Gene Isolated from Indonesian Local Tobacco (Nicotiana tabacum cv. Sindoro1) SESANTI BASUKI; NURHAJATI MATTJIK; DESTA WIRNAS; . SUWARSO; . SUDARSONO
HAYATI Journal of Biosciences Vol. 20 No. 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

Nicotine is the major alkaloid compound in cultivated tobacco (Nicotiana tabacum) that could potentially be converted into carcinogenic compound (nor-nicotine). The PMT gene encoding putrescine N-methyltransferase (PMT) is one of the two key genes that play a prominent role in nicotine biosynthesis. The aimed of this study was to isolate and characterize the cDNA sequence originated from Indonesian local tobacco cv. Sindoro1 (Ntpmt_Sindoro1). The results showed that the Ntpmt_Sindoro1 was 1124 bp in length. This cDNA fragment encodes for 374 amino acid residues. The predicted polypeptide from the cDNA is a hidrophilic protein, and has a predicted molecular weight of 40.95 kDa. The predicted amino acids sequence also showed high similarity to the PMT gene product Nicotiana sp. available in the GenBank data base. The amino acid sequences also exert conserved residues specifically exhibited only by PMT gene originated from N. tabacum. Clustering analysis revealed that Ntpmt_Sindoro1 belongs to the same clade as the PMT3 gene, a member of the N. tabacum PMT gene family. The Ntpmt_Sindoro1 cDNA sequence covering exon1-exon8 of the PMT gene fragment has been registered in the GenBank data base, under the accession number JX978277.

Perluasan Indeks Seleksi Nilai Fenotipe Untuk Indeks Seleksi Nilai Pemuliaan Edizon Jambormias; Surjono Hadi Sutjahjo; Ahmad Ansori Mattjik; Yudiwanti Wahyu; Desta Wirnas
Buletin Agrohorti Vol. 2 No. 1 (2014): Januari 2014
Publisher : Departemen Agronomi dan Hortikultura

Indeks seleksi merupakan salah satu prosedur seleksi sifat berganda yang penting dalam program pemuliaan tanaman. Tulisan ini bertujuan untuk mendeskripsi dan menduga parameter-parameter skor indeks dari metode-metode indeks seleksi nilai fenotipe, dan kemungkinan perluasannya bagi metode indeks seleksi nilai pemuliaan BLUP. Peragam fenotipe dan genotipe dari indeks basis dan Smith-Hazel digunakan sebagai landasan statistik untuk pendugaan parameter dan perluasan penggunaan indeks. Penurunan rumus menghasilkan peragam aditif dan fenotipe bagi indeks seleksi berbasis nilai eigen, dan memperoleh parameter indeks seperti heritabilitas, koefisien determinasi, respons seleksi, respons seleksi tak langsung dan respons komponen sifat-sifat kuantitatif bagi semua metode indeks nilai fenotipe. Parameter peragam nilai pemuliaan sebenarnya dapat diduga dari analisis eigen dan keakuratan nilai pemuliaan BLUP yang memungkinkan metode indeks seleksi nilai fenotipe dapat diterapkan dengan prosedur yang sama pada nilai pemuliaan BLUP. Terakhir, heritabilitas dan koefisien determinasi indeks dapat digunakan dalam simulasi pembobot ekonomis untuk menghasilkan indeks terbaik.

Potensi Hasil Galur – Galur Harapan Kedelai Hitam (Glycine max ( L.) Merr.) di Kabupaten Bogor Lela Marlenasari; Desta Wirnas
Buletin Agrohorti Vol. 3 No. 2 (2015): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Tujuan dari penelitian ini adalah untuk mengevaluasi potensi hasil dari 8 galur harapan kedelai hitam (Glycine max (L.) Merr.). Penelitian ini dilakukan di Bogor (Jawa Barat) pada bulan Oktober 2011 sampai Januari 2012 sebagai bagian dari uji coba multilokasi. Rancangan percobaan yang digunakan adalah rancangan kelompok lengkap teracak dengan pengulangan dan tiga varietas nasional sebagai kontrol, yaitu Cikuray, Malika dan Willis. Hasil penelitian menunjukkan bahwa tinggi tanaman, waktu berbunga dan waktu panen, jumlah cabang produktif dan jumlah polong hampa sceara statistik berbeda. SSD-82, SSD-91, dan SC-68-2 memiliki produktivitas tinggi dibandingkan varietas pembanding (Cikuray).

Co-Authors ,, Mawaddah . SUDARSONO . SUWARSO Achmad . Adinda Wuriandani Adinda Wuriandani Agung Wahyu Susilo Agung Wahyu Susilo Agung Wahyu Susilo Ahmad Ansori Mattjik Ahmad Ansori Mattjik AHMAD JUNAEDI Aidi Noor Akhmadi, Gerland Alaydi, Nafian Amris Makmur Arvita Netti Sihaloho, Arvita Netti Atika Bakti Sari Azri Kusuma Dewi Bambang Sapta Purwoko Buang Abdullah Dedek hanafiah Dewi Andriani Diana Sofia Hanafiah Didy Sopandie Edi Santosa Edison Jambormias EDIZON JAMBORMIAS Eka Bobby Febrianto, Eka Bobby Eni Widajati Faqih Udin Faradila Median Rini Firman Ardiansyah Gopar, R. A. HAJRIAL ASWIDINNOOR Halimah Tus Sa'diah Halimatus Syahdia Hasibuan HESTI MAULIDA Hidayatun, Nurul Human, dan Soeranto I. Prihantoro, I. Imam Widodo Ishmatalhaq, Zannuba Iskandar Lubis Isnaini Isnaini Iswari S. Dewi Iswari Saraswati Dewi Jaisyurahman, Usamah Jasmi Jekki Irawan Junaedi, dan Ahmad Karlin Agustina Khairil Anwar L. Abdullah Lela Marlenasari M A Chozin Mahpuzah, Marfiatun Manalu, Victor Manotar Pademan Mardhiyyah, Rodhiyatan Marlenasari, Lela Maryono, Marina Yuniawati Mattjik, Ahmad A Maulida, Hesti Mayang Sari Memen Surahman Mizan, Muhammad Rauful Mizan, Muhammad Rauful Momongan, Jorex Daniel Muhamad Syukur Muhammad Arif Yudiarto Munarti Munif Ghulamahdi Nindita, Anggi Nita Kartina, Nita Nofika Senjaya NUR KHOLISOH NURHAJATI AA MATTJIK NURHAJATI MATTJIK Nurheni Wijayanto OTIH ROSTIANA P. D. M. H. Karti Patty, Jacob R Pipit Werdhiwati Prasetiyono, dan Joko Prasetiyono, Joko Pratama, Muhammad Antony Jefri Purbokurniawan . Putra, B. Putri Andini Mandasari Ratih Irma Khairani Saragih Rini, Erin Puspita Rini, Faradila Median S. Human Saniaty, Arina Saragih, Ery Leonardo Saragih, Ratih Irma Khairani Sayurandi Sayurandi Sayurandi Sayurandi Sayurandi Sayurandi Sayurandi Sayurandi, Sayurandi Saﬁtri, Heni Sekar Woelan Sekar Woelan Sekar Woelan Sekar Woelan SESANTI BASUKI SESANTI BASUKI Sherly Rahayu Siti Marwiyah Siti Marwiyah Sobir Sobir Sobir Sobir Sobir Sobir Soeranto Human SRI WARDANI Sudarsono SUDARSONO SUDARSONO Sudarsono, dan Sudirman Yahya Sudirman Yahya Sudirman Yahya Sudirman Yahya - Sumiati, dan Sungkono Sungkono Sunyoto Sunyoto Surjono H Sutjahjo, Surjono H Surjono Hadi Sutjahjo Suwarno, Punjung Medaraji SUWARSO SUWARSO Tasliah, nFN Tias Arlianti Tri Budiyanti Trikoesoemaningtyas Trikoesoemaningtyas Trikoesoemaningtyas Trikosoemaningtyas Trikosoemaningtyas Trikosoemaningtyas Usamah Jaisyurahman W. Puspitasari Wage Ratna Rohaeni WAGE RATNA ROHAENI Wardhani, Azhahara Putri Kusuma Wibawa, Rentang Fajar Cakra Wuriandani, Adinda Yani Nurhadryani Yudiwanti Wahyu E. Kusumo Yulidar Yulidar

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