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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Media Pertanian
Saepudin, Adam
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Pengaruh Pupuk Guano dan POC Daun Gamal Terhadap Pertumbuhan Vegetatif Tanaman Tomat (Lycopersicum esculentum Mill) Nurfitriani, Mieta; Suhardjadinata, Suhardjadinata; Saepudin, Adam
Media Pertanian Vol 10, No 1 (2025): Media Pertanian
Publisher : Program Studi Agroteknologi Universitas Siliwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37058/mp.v10i1.12925

Abstract

Tomat (Lycopersicum esculentum Mill) adalah salah satu jenis sayuran yang sangat disukai di Indonesia karena buah tomat memiliki nilai gizi yang tinggi sebagai sumber vitamin A, B dan C. Jadi, dalam upaya peningkatan produktivitas tanaman tomat dilakukan pemberian pupuk guano dan POC daun gamal. Rancangan uji yang diterapkan adalah rancangan kelompok acak (RKA) faktorial yang melibatkan 2 variabel dan 3 pengulangan. Faktor pertama, empat taraf konsentrasi POC daun gamal yaitu g0 = kontrol (tanpa POC), g1 = konsentrasi POC 100 L-1, g2 = konsentrasi POC 200 L-1, g3 = konsentrasi POC 300 L-1. Faktor kedua, empat taraf dosis pupuk guano yaitu a1 = 5 t ha-1, a2 = 10 t ha-1, a3 = 15 t ha-1, a4 = 20 t ha-1. Penelitian ini menunjukkan adanya keterkaitan antara jumlah pupuk guano dan kadar POC pada daun gamal berkaitan dengan tinggi tanaman, area daun, laju asimilasi bersih (LAB), serta laju pertumbuhan tanaman (LTT) pada berbagai usia pengamatan tomat. Guano 10 t ha-1 dengan POC pada konsentrasi 200 ml L-1, dan  guano 15 t ha-1 dengan POC daun gamal pada konsentrasi 100 ml L-1menghasilkan pertumbuhan vegetatif tomat  terbaik.
Induksi dan Proliferasi Embriogenesis Somatik In Vitro pada Lima Genotipe Kedelai Saepudin, Adam; Khumaida, Nurul; Sopandie, Didy; Ardie, dan Sintho Wahyuning
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.896 KB) | DOI: 10.24831/jai.v44i3.12782

Abstract

ABSTRACTSomatic embryo induction medium was reported to be genotype dependent for soybean. This study was aimed to obtain the optimum medium for embryo somatic induction and proliferation, and to regenerate somatic embryo of five soybean genotypes. Five soybean genotypes (Tanggamus, Anjasmoro, Yellow Biloxi, CG-22-10, and SP-10-4) were used in this study. The research was divided into four steps: (1) embryogenic callus induction of  five soybean genotypes, (2) embryogenic callus proliferation of five soybean genotypes, (3) optimation of embryo somatic induction on five soybean genotypes and (4) embryo somatic regeneration of five soybean genotypes. The induction experiment showed that based on number of embryogenic callus, the best somatic embryo-induction medium was 3% sucrose+ NAA 5 mg L-1+2,4-D 5 mg L-1+ Vitamin B5. Embryogenic callus number for each genotype tested was increased on proliferation media of 3% sukrosa + 2,4-D 5 mg L-1 + NAA 5 mg L-1+ Vit B5, and Yellow Biloxi gave the highest number of proliferated somatic embryos compared to other genotypes. Increasing number of globular somatic embryo of all genotypes was obtained from the optimation of somatic embryo induction media being used, and Tanggamus genotype gave the highest number of globular somatic embryo which followed by Yellow Biloxi genotype. Tanggamus and Yellow Biloxi genotypes were also successfully formed the four steps of somatic embryos (globular, heart, torpedo, and cotyledonary stages), but in regeneration medium of MS0 and media MS + sukrosa 10 g L-1 + GA3 2 mg L-1 + BAP 4 mg L-1 + Vit B5 only Tanggamus genotype was regenerated into plantlet.  Keywords: 2,4-D, NAA, somatic embryos, induction, proliferation
In Vitro Selection of Four Soybean Genotypes using PEG for Drought Tolerance Saepudin, Adam; Khumaida, Nurul; Sopandie, Didy; Ardie, and Sintho Wahyuning
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.318 KB) | DOI: 10.24831/jai.v45i1.13749

Abstract

The use of somatic embryo for in vitro selection program is very useful since the selected traits will be inherited in the progeny. This study was aimed to obtain somaclonal variants for drought tolerances of soybean genotypes from in vitro selection using polyethylene glycol (PEG). The experiments were set up in two factors with completely randomize design and each treatment was replicated ten times. Four soybean genotypes (Tanggamus, Anjasmoro, Yellow Biloxi, CG-22-10, and SP-10-4) were used in this study as first factor experiment. In vitro selection was in embryogenic callus level using media containing PEG (0, 5, 10, 15, and 20%) as second factor experiment was done to all genotypes. The results showed that after 3 months in the selection medium, SP-10-4 and Tanggamus genotypes showed higher percentage of fresh callus (surviving callus) and number of embryogenic callus, compared to Anjasmoro and Yellow biloxi. In contrast, CG-22-10 had the lowest number of fresh callus and number of embryogenic callus compared to other genotypes. PEG in high concentration decreased the percentage of fresh callus and number of embryogenic callus in all genotypes. Tanggamus was the only genotype that survived until cotyledonary-stage embryos after transferring in MS0 regeneration medium. Seven Tanggamus somatic embryos from PEG selection succesfully germinated and regenerated into plantlet as drought-tolerant somaclone candidates. Keywords: abiotic stress, embryogenic callus, Glycine max, somaclone
Co-Authors Ardie, and Sintho Wahyuning Ardie, dan Sintho Wahyuning Didy Sopandie Nurfitriani, Mieta Nurul Khumaida Suhardjadinata, Suhardjadinata