<![CDATA[Pain is an uncomfortable sensory and emotional feeling for the sufferer, and pain arises as a sign of tissue damage. There are various types of analgesic drugs used by the community, both traditional and synthetic. One of the plants used as a traditional medicine is Seligi (Phyllanthus buxifolius (Blume) Muell. Arg). The leaves of Seligi are known to contain flavonoid compounds that inhibit prostaglandin biosynthesis, a process that produces pain mediators. The purpose of this study is to examine the analgesic effects of the ethanol fraction of Seligi leaves on mice and to establish the optimal dose for oral analgesia in mice. In this investigation, the Sigmund approach was employed. The test animals were 25 male white mice divided into five groups: the negative control group (carboxymethyl cellulose), the positive control group (mefenamic acid) at a dose of 65 mg/kg body weight, and Groups I, II, and III, which were given the ethanol fraction of celosia leaves orally at doses of 46 mg/kg body weight, 92 mg/kg body weight, and 138 mg/kg body weight. After 15 minutes, the mice were intraperitoneally injected with 1% acetic acid, and their movements were observed and counted every 5 minutes for 1 hour. The research results revealed that Group I provided a protection percentage of 36.63% and an effectiveness percentage of 52.3%. Group II provided a protection percentage of 62.98% and an effectiveness percentage of 85.26%. Group III achieved a protection percentage of 71.1% and an effectiveness percentage of 96.26%, which is nearly comparable to the control group, which achieved a protection percentage of 73.86% and an effectiveness percentage of 100%. The results of the smallest significant difference test showed that the positive controls (doses 1, 2, and 3) were significantly different from the negative control, indicating that these groups exhibited an analgesic effect. At doses 2 and 3, there was no significant difference from the positive control, indicating that these doses are equivalent to the positive control.]]>
