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All Journal Journal of Microbiology, Biotechnology and Conservation
Fatahillah Ardhani
University of Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology

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Analisis Hubungan Kekerabatan Famili Felidae (Ordo Karnivora; Kelas Mamalia) Berdasarkan Sekuens Gen Cytochrome-B (CytB) Mitokondria Fatahillah Ardhani; Ida Ayu Dewi Lola Valastri; Khusnul Khotimah; Faturrahman
Journal of Microbiology, Biotechnology and Conservation Vol. 2 No. 2 (2026): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/v6twrz55

Abstract

Species identification within the family Felidae is often challenged by overlapping morphological characteristics resulting from hybridization, necessitating the use of genetic approaches. This study aimed to analyze the phylogenetic relationships and evolutionary history of 40 Felidae species based on mitochondrial Cytochrome b (CytB) gene sequences. This in silico study utilized secondary data obtained from GenBank, which were analyzed using MEGA 12 for sequence alignment, nucleotide composition analysis, and phylogenetic tree reconstruction using the Maximum Likelihood method with 1,000 bootstrap replicates, followed by validation using DrPhylo. The results showed a predominance of transition mutations, with a high R ratio (9), as well as significant codon usage bias. Phylogenetic reconstruction divided the family Felidae into eight major clades supported by high bootstrap values, namely Panthera, Neofelis, Pardofelis, Prionailurus, Lynx, Leopardus, Felis, and a combined Puma group. The genus Otocolobus was identified as the oldest lineage, diverging earliest from the other lineages. The M-Grid test confirmed the validity of the major clade groupings, with confidence levels approaching 1.00. It can be concluded that the CytB gene is an effective and reliable genetic marker for phylogenetic studies of the family Felidae.
Co-Authors Faturrahman Ida Ayu Dewi Lola Valastri Khusnul Khotimah