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All Journal Indonesian Journal for Health Sciences (IJHS) Jaringan Laboratorium Medis
Oos Fatimah Rosyati
Poltekkes Kemenkes Bandung
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Nursing Public Health

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Comparison Of Middlebrook 7H9 Culture and Xpert MTB/XDR for Detecting Isoniazid and Fluoroquinolone Resistance Rika Rostikawati; Hafizah Ilmi Sufa; Oos Fatimah Rosyati; Asep Dermawan
Indonesian Journal of Health Science Vol 10 No 02 (2026): September
Publisher : Universitas Muhammadiyah Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24269/ijhs.v10i02.13441

Abstract

Drug-resistant tuberculosis (DR-TB) remains a major public health challenge in Indonesia, necessitating rapid and accurate detection of resistance to key anti-tuberculosis drugs. This quantitative descriptive analytic cross-sectional study evaluated the concordance of Mycobacterium tuberculosis resistance detection between phenotypic Middlebrook 7H9 liquid culture and genotypic TCM XDR in 44 DR-TB patients, focusing on isoniazid (H) and fluoroquinolone/levofloxacin (FQ/Lfx) resistance. Data were analyzed using the McNemar test methode. High-concentration H resistance was detected in 68.2% by culture and 65.9% by TCM XDR, while FQ/Lfx resistance was identified in 18.2% and 15.9%, respectively. No significant differences were observed between methods for high-concentration H and FQ/Lfx resistance detection (P=1.000), indicating high concordance with cappa values 0,74 isoniazid for and 0,86 for FQ/Lfx resistance. In contrast, a significant discrepancy was found for low-concentration H resistance (P=0.001), with a discordance rate of 25%, potentially attributable to HA promoter mutations not included in the TCM XDR panel or non-genetic adaptive mechanisms.
Antibiotic Susceptibility Profile of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Diabetic Foot Wounds Yasmin Husnul Khotimah; Oos Fatimah Rosyati; Hafizah Ilmi Sufa; Ira Gustira Rahayu
Jaringan Laboratorium Medis Vol 8 No 2 (2026): November 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i2.15694

Abstract

Diabetic foot wound infection is a complication of diabetes mellitus that can be caused by various bacteria, including Methicillin-Resistant Staphylococcus aureus (MRSA), which may complicate the selection of antibiotic therapy because of its high level of resistance. This study aimed to identify MRSA isolates and determine their antibiotic susceptibility profiles from diabetic foot wounds of patients with diabetes mellitus at RUMAT Diabetes Wound Care, Bandung City. This descriptive laboratory experimental study used 30 diabetic foot wound samples collected from patients with diabetes mellitus. The isolates were identified by culture on Blood Plate Agar and Mannitol Salt Agar, followed by Gram staining, catalase test, coagulase test, and MRSA screening using cefoxitin discs. Antibiotic susceptibility testing was performed using the Kirby–Bauer disk diffusion method on Mueller–Hinton Agar according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The results showed that 7 (23.3%) isolates were identified as MRSA. All MRSA isolates were obtained from patients with type 2 diabetes mellitus who had been diagnosed with diabetes for at least one year. The MRSA isolates exhibited 100% susceptibility to azithromycin and amikacin; 85.7% susceptibility to tetracycline, gentamicin, ciprofloxacin, levofloxacin, and trimethoprim-sulfamethoxazole; 71.4% susceptibility to amoxicillin, erythromycin, and clindamycin; and 57.1% susceptibility to chloramphenicol. These antibiotic susceptibility profiles may provide useful information to support the selection of antibiotic therapy for diabetic foot wound infections in Bandung City.
Co-Authors Asep Dermawan Hafizah Ilmi Sufa Ira Gustira Rahayu Rika Rostikawati Yasmin Husnul Khotimah