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The Effect of Local Organic Fertilizer from Rubber Processing Waste on the Yield of Rubber Plants Simanjuntak, Ernawati; Prasetyo, Prasetyo; Hartal, Hartal
Akta Agrosia Vol 21 No 1 (2018)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (276.501 KB)

Abstract

Rubber plant is an important industrial crop, as for economic community and a source of non-oil foreign exchange for the country.  In Indonesia, 85% rubber planting area is community rubber plantation with its productivity is still very low (700 - 900 kg ha-1 year-1), compared to its potential which can be more than 1,500 kg ha-1 year-1.  The low productivity of community rubber plantation is because of the lack of proper handling and maintenance of their plants.  Efforts is needed to improve the quality and productivity of community rubber plantation so as to compete in international trade by improvement on planting, maintenance, latex post-harvesting from the garden to the final processing stage.  This research utilizes solid waste from rubber processing factory that is local organic fertilizer (LOF) which is usually merely dumped and become source of environment contaminant.  The objective of this study was to determine the optimum dosage of rubber mill waste to the yield of rubber plants.  The research was carried out at farmer’s plantation of Tanjung Tengah village, Sub-district of Pondok Kelapa, Central Bengkulu, from May to August 2016.  The experiment was arranged in a Randomized Complete Block Design (RCBD) with four replications and 6 levels of LOF as the treatments i.e. 0, 10, 20, 30, 40 and 50 kg tree-1 equal to 0, 5, 10, 15, 20 and 25 tons ha-1, respectively.  The results showed that the effect of several doses of organic fertilizer of rubber waste to rubber plants had no effect on latex volume, stem girth, latex weights, latex slab weight, and dry weight.  The yield of rubber even tended to decrease as the increase of dose of LOF.  This was supposedly because the rubber plant is an annual crop so takes a long time to show the response of the treatment.Keywords: rubber sap, waste, compost fertilizer
Establishment of Reference Value of 20 Amino Acids for Toddlers by High Performance Liquid Chromatography Tandem Mass Spectrometry Pasaribu, Merci Monica br; Immanuel, Suzanna; Sjarif, Damayanti Rusli; Timan, Ina Susianti; Malik, Safarina G.; Mansyur, Muchtaruddin; Simanjuntak, Ernawati
The Indonesian Biomedical Journal Vol 16, No 2 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i2.2902

Abstract

BACKGROUND: Amino acids are one of the essential metabolites, especially the 20 amino acids that are preserved as the building blocks of protein. Alterations in amino acid concentrations are related to disease such as inborn error of metabolism, cancer, as well as nutritional status. Hence, it is necessary to define reference values of 20 plasma-free amino acids for Indonesian toddlers and to establish a robust measurement technique using chromatography with tandem mass spectrometry (MS).METHODS: This study was a cross-sectional preliminary study to establish reference values. The sample was prepared by mixing plasma with 20% sulfosalicylic acid. Plasma-free amino acids were measured with high-performance liquid chromatography (HPLC) non-derivatization technique using column XTerra for chromatographic separation coupled with tandem MS. Amino acids reference values were taken from 101 healthy Indonesian toddlers aged 1-3 years old. Since amino acids data were not Gaussian distributed, the lower and upper of the reference value was established from the 5th percentile and the 95th percentile, respectively.RESULTS: Analysis for 20 amino acids was validated. The accuracy ranged from 90.53-105.39% and the precision ranged from 0.06-3.80%. The limit of detection range was 1-2 nmol/mL, and the limit of quantification range was 2-4 nmol/mL. The result was linear, with R2 higher than 0.998. There was no significant difference between boys and girls for all amino acids except for glycine.CONCLUSION: HPLC with tandem MS method can be used to evaluate amino acids in clinical practice. The reference values obtained are specific for aged 1-3 years old from urban areas in Indonesia. The study suggests that for each population, the reference values for amino acids should be established.KEYWORDS: amino acids, high-performance liquid chromatography, tandem mass spectrometry, reference values, Indonesia