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All Journal EUGENIA
Edy Fredy Lengkong
Program Studi Agronomi, Fakultas Pertanian, Universitas Sam Ratulangi
Agriculture, Biological Sciences & Forestry

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PENGARUH KONSENTRASI ZAT PENGATUR TUMBUH THIDIAZURON (TDZ) TERHADAP PERTUMBUHAN PLANLET KENTANG INDUSTRI Praiselia Majesty Sahoa; Edy Fredy Lengkong; Jeane Sukarni Marhaeni Raintung; Johannes E. Xaveriano Rogi; Maria Goretti M. Polii; Annatje Engelien B. Inkiriwang
EUGENIA Vol. 31 No. 2 (2025): Eugenia
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.v31i2.65461

Abstract

This study aimed to determine the effect of Thidiazuron (TDZ) growth regulator concentrations on the in vitro growth of industrial potato plantlets from two cultivars, Ventury Agrihorti and Golden Agrihorti. The research was conducted at the Biotechnology Laboratory, Faculty of Agriculture, Sam Ratulangi University, Manado, using a factorial Completely Randomized Design (CRD) consisting of two factors: cultivar (V1 = Ventury, V2 = Golden) and TDZ concentration (0, 1, 2, 3, and 4 ppm), each with five replications. The observed variables included the number of leaves, number of shoots, plant height, and dry weight. The results showed that TDZ concentration significantly affected plantlet growth, particularly the number of shoots, which increased significantly in the Ventury Agrihorti cultivar at 1 ppm TDZ. The treatment without TDZ (control) produced the best results for plant height, number of leaves, and dry weight. The Ventury Agrihorti cultivar produced higher dry weight than Golden Agrihorti, while Golden Agrihorti exhibited better plant height. The negative effects of TDZ were presumed to be due to the decrease in medium pH after autoclaving, which affected hormone effectiveness and nutrient stability in the medium. Keywords: Thidiazuron, industrial potato, tissue culture, plant growth regulator, Solanum tuberosum.
KARAKTERISTIK BEBERAPA VARIETAS KANGKUNG DARAT (Ipomoea reptans Poir): Characteristics of Several Varieties of Upland Water Spinach (Ipomoea reptans Poir) Ivana Manopo; Jantje Pongoh; Annatje E B Inkiriwang; Edy Fredy Lengkong; Sesilia Anita Wanget; Langimanapa Sofia Demmassabu
EUGENIA Vol. 32 No. 1 (2026): Eugenia
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/07cs7d30

Abstract

ABSTRACT Upland water spinach (Ipomoea reptans, Poir) is a widely consumed leafy vegetable due to its good nutritional content, rapid growth, and ease of cultivation; however, its productivity often varies depending on the characteristics of the varieties used. This study aimed to determine the growth characteristics of five upland water spinach varieties, namely Bisi, Bangkok LP-1, Rajawali, Serimpi, and Bika. The research was conducted in Kalasey Satu Village, Mandolang District, Minahasa Regency from June to July 2025 using a Randomized Block Design (RBD) with five treatments and three replications, totaling 15 experimental units. Observations were carried out at 25, 30, and 35 days after planting (DAP) on plant height, number of leaves, number of branches, stem diameter, and fresh weight. The results showed no significant differences among varieties at 25 and 30 DAP, while significant differences occurred at 35 DAP. The Bika variety exhibited the best growth performance with an average plant height of 49.56 cm, 57.11 leaves, 8.78 branches, 8.52 mm stem diameter, and 48.33 g fresh weight, followed by Serimpi which also showed relatively high vegetative growth. The Bisi variety had a larger stem diameter but lower leaf and branch numbers, while Bangkok LP-1 and Rajawali displayed lower overall growth and yield. Based on these results, the Bika variety is recommended for cultivation due to its superior growth characteristics, and further studies are suggested to evaluate nutritional quality and determine optimal harvest timing to ensure both high yield and desirable consumption quality. Keywords: land spinach, varieties, characteristics, water, horticulture
Co-Authors Annatje E B Inkiriwang Annatje Engelien B. Inkiriwang Ivana Manopo Jantje Pongoh Jeane Sukarni Marhaeni Raintung Johannes E. Xaveriano Rogi Langimanapa Sofia Demmassabu Maria Goretti M. Polii Praiselia Majesty Sahoa Sesilia Anita Wanget