Burnout has become a critical occupational health issue due to its adverse psychological and physical consequences, as well as its negative impact on employee performance. Burnout develops as a form of chronic work-related stress resulting from excessive job demands, high performance targets, and an imbalance between job demands and available resources. This condition may reduce job satisfaction and increase work stress, ultimately affecting both employees and organizations. This research examines the impact of burnout on employees’ work stress by incorporating job satisfaction as a mediating construct among staff members of PT XYZ Semarang. The study applies a quantitative explanatory approach. The sample comprises 35 employees who were chosen through a random sampling method. Data collection was conducted using a Likert-type questionnaire, and the data were subsequently processed using Partial Least Squares–based Structural Equation Modeling with the assistance of SmartPLS software. The findings demonstrate that burnout exerts a statistically significant influence on both job satisfaction and work stress. Burnout directly increases work stress and significantly reduces job satisfaction, while job satisfaction has a negative effect on work stress and partially mediates the relationship between burnout and work stress. These findings suggest that job satisfaction plays a protective role in reducing the negative impact of burnout on employee stress. The practical implications of this study highlight the importance of organizational strategies that focus on managing burnout, enhancing job satisfaction, and creating supportive work environments to promote employee well-being and sustainable organizational performance.