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All Journal AGRIVITA, Journal of Agricultural Science BIOVALENTIA: Biological Research Journal
Wendra, Fahmi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

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CANDIDATE GENES IDENTIFICATION OF OIL PALM (Elaeis guineensis Jacq.) INTEREST CHARACTERS USING PUBLISHED DATABASE Sarimana, Upit; Putro Priadi, Dwi; Hasmeda, Mery; Erika, Pratiwi; Herrero, Javier; Santika, Baitha; Wendra, Fahmi; Sembiring, Zulhermana; Asmono, Dwi
BIOVALENTIA: Biological Research Journal Vol. 7 No. 1 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.969 KB) | DOI: 10.24233/biov.7.1.2021.188

Abstract

Palm oil has supplied more than 30% of vegetable oil consumption worldwide. Rising demand has pushed oil palm plantations to increase the yield. It is well known that genetic has played a significant role in phenotypic performance. Moreover, in recent years, genomic data has emerged tremendously. Unfortunately on the gene related to oil palm yield. Therefore, a preliminary study to classify and select oil palm candidate genes associated with characteristic by scanning existing genes in oil palm or other in-silico species were conducted. Based on Blast2Go results, 22 genes related to oil biosynthesis, two specifically related to fruit number and fruit weight were analysed. Furthermore, 19 candidate genes were able to amplify.
THE EFFECT OF CULTURE MEDIA TYPE AND PLANT GROWTH REGULATORS ON CALLUS INDUCTION OF OIL PALM (Elaeis guineensis Jacq) PISIFERA TYPE Sulaksono, Gogoh; Hasmeda, Mery; Hanum, Laila; Wendra, Fahmi; Santika, Baitha; Asmono, Dwi
BIOVALENTIA: Biological Research Journal Vol. 7 No. 2 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (977 KB) | DOI: 10.24233/biov.7.2.2021.224

Abstract

Oil palm has a high economic value since it is one of the highest vegetable oil-producing plants compared to other oil-producing plants. The provision of good planting material for oil palm plantations is one of the determining factors to increase productivity. The parent Pisifera type of oil palm is important to develop the high-yielding oil palm Tenera varieties. The provision of oil palm material could be done through generative system with seeds and also could be done through vegetative approaches using tissue culture techniques. It aims to get plants that are genetically the same as their parents (true to type). The purpose of this study was to see the effect of different type of tissue culture media and plant growth regulators (PGR’s) on the callus induction stage of Pisifera type oil palm. The results show that the treatment using Murasige and Skoog (MS) culture media is able to give a better effect when compared to the use of Eeuwens and Blake (Y3) basic media. While the use of PGR treatment (H6 = NAA 6 mg/l + 2.4-D 0.5 mg/l) showed a callus growth percentage of 76%, which was better than (H1 = Kin 0.1 mg/l + 2.4-D 100 mg/l) by 42%.
Effect of Media and Plant Growth Regulator on Proliferation of Somatic Embryogenic Callus of Oil Palm (Elaeis gueenesis Jacq.) Sulaksono, Gogoh; Hasmeda, Mery; Hanum, Laila; Wendra, Fahmi; Santika, Baitha
AGRIVITA Journal of Agricultural Science Vol 46, No 2 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i2.4234

Abstract

The Pisifera type oil palm is an important palm variety and mostly used as a pollen source to produce Tenera commercial hybrid varieties. This research on proliferating fertile Pisifera oil palm callus using somatic embryogenesis method was conducted at the Tissue Culture Laboratory of PT. Sampoerna Agro, Tbk from February to August 2021 to find out the optimum basal media and the concentration of growth regulators on callus proliferation. The planting material was the primary callus from the previous research. This study used a factorial Completely Randomized Design with two factors. The first factor involved two types of basal media: MS and Y3, and the second factor consisted of different concentrations of growth regulators (PGR). All treatments were repeated three times. The results showed that the use of MS media supplemented with 6 mg/l NAA and 0.5 mg/l 2,4-D was the most effective treatment for the proliferation of embryogenic callus in term of callus initiation time, the capacity of primary callus formation, primary calli morphology and proliferation, and callus fresh weight.
Co-Authors Dwi Asmono Dwi Putro Priadi Erika, Pratiwi Herrero, Javier Laila Hanum Mery Hasmeda Santika, Baitha Sarimana, Upit Sembiring, Zulhermana Sulaksono, Gogoh