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All Journal Biospecies Jurnal Lahan Suboptimal Biosfer: Jurnal Tadris Biologi Proceeding International Conference on Global Resource Conservation Indonesian Journal of Biotechnology Biology, Medicine, & Natural Product Chemistry Journal of Tropical Biodiversity and Biotechnology Proceeding of the Electrical Engineering Computer Science and Informatics Science and Technology Indonesia Jurnal Penelitian Sains DIDAKTIKA BIOLOGI: Jurnal Penelitian Pendidikan Biologi IJEMS (Indonesian Journal of Environmental Management and Sustainability) Riau Journal of Empowerment Edumaspul: Jurnal Pendidikan Seminar Nasional Lahan Suboptimal Jurnal Gema Ngabdi Jurnal Vokasi Journal of Environmental Science and Sustainable Development Berkala Penelitian Hayati Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat SRIWIJAYA JOURNAL OF ENVIRONMENT BIOVALENTIA: Biological Research Journal Jurnal Pepadu JURNAL BIOLOGI PAPUA Dikdimas : Jurnal Pengabdian Kepada Masyarakat Bulletin of Chemical Reaction Engineering & Catalysis Medicosphere Aksi Kita: Jurnal Pengabdian Kepada Masyarakat

Laila Hanum

Biology Department, Faculty Of Mathematics And Natural Sciences, Sriwijaya University, Indralaya, 30662, Indonesia

Author-ID : 274831

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Genetic Relatedness among Duku, Kokosan, and Pisitan in Indonesia Based on Random Amplified Polymorphic DNA Markers Hanum, Laila; Kasiamdari, Rina Sri; ., Santosa; ., Rugayah
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

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Genetic relatedness among duku, kokosan, and pisitan from Indonesia were investigated using random amplified polymorphic DNA (RAPD) markers. Eleven primers (OPA-01, OPA-02, OPA-10, OPB-07, OPB-11, OPB-12, OPB-15, OPT-16, OPU-14, OPU-19, and OPU-20) were used for amplification and yielded a total of 174 DNA bands, of which 167 were polymorphic. Primer OPA-10, OPB-11, OPB-12, OPB-15, and OPU-19 produced all of the polymorphic DNA bands. The size of the amplified DNA fragments ranged from 41-1546 bp. The dendrogram separated into two clusters at a genetic similarity coefficient of 0.76. The cluster 1 consisted of subclusters duku and several pisitan (pisitan OKI, pisitan Sleman, pisitan Hatu, pisitan Punggur, and pisitan Tanjung), and cluster 2 consisted of subclusters kokosan and pisitan. In the kokosan subclusters, including duku Drendan. Dendrogram supported the determination of taxonomic status of duku, kokosan, and pisitan as one species, namely Lansium domesticum Corr. and its divided into two groups, namely L. domesticum ’duku group’ and L. domesticum ’pisitan-kokosan group’. Thus, RAPD analysis was useful tool for determining the genetic variation and the genetics relatedness among duku, kokosan, and pisitan in Indonesia.Key words: duku, kokosan, pisitan/langsat, genetic relatedness, RAPD

The Phylogenetic Relationship Among Varieties of Lansium domesticum Correa Based on ITS rDNA Sequences Hanum, Laila; Kasiamdari, Rina Sri; ., Santosa; ., Rugayah
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

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Lansium domesticum Corr. with vernacular name in Indonesian duku has been reported containingtherapeutic bioactive compounds, and some of these compounds shown to be potent antitumor, anticancer,antimalaria, antimelanogenesis, antibacteria, and antimutagenic activities. This plant is commonly known asduku, kokosan and langsat by the local community in Indonesia. The morphological appearance of all varieties isnearly the same, and identifi cation of the varieties is very diffi cult for growers. Variation of DNA sequences ofthe ITS (Internal transcribed spacer) region can be used as a molecular character to determine the phylogeneticrelationship of different varieties of L. domesticum. The aims of this study were to determine taxonomy status ofduku, kokosan, and langsat, also phylogenetic relationship among varieties of L. domesticum based on ITS rDNAsequencing. DNA was isolated from leaves of plant and then amplifi ed using F1 and R1 primers. Nucleotidesequences were identifi ed using Sequence Scanner Software Programm version 1.0, nucleotide sequences from18S, ITS1, 5.8S, ITS2 and 26S region, that has been mergered using EditSeq and SegMan in software Suite forSequence Analysis DNASTAR Lasergene DM version 3.0.25. The results of study showed that DNA fragmentsranging in size from 782-810 bp. Different pattern of DNA fragments indicated polymorphism among duku,kokosan, and langsat. Based on the results of the ITS rDNA sequencing and phylogenetic tree analysis. Itwas determined that Lansium and Aglaia are a separated genus with the similarity index value of 0.98. Duku,kokosan and langsat were divided into two cluster, namely cluster kokosan-langsat and cluster duku with thesimilarity index value of 0.996.Keywords : Phylogenetic relationship, ITS region, L. domesticum, duku, kokosan, langsat

Growth of generative explants of Moringa oleifera on different culture conditions Muslihatin, Wirdhatul; Rizkia, Hanum Laila
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

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In vitro culture is more effective propagation technique because the propagation time is faster than conventional propagation Â so the achievement of the results is Â in favor of the improvement program and faster Â spread of Moringa oleifera. The objectives of this study were to determine the effect of carbon source and photoperiod on the growth ofÂ generative explants of M. oleifera in vitro. The method performed were inoculation of generative explans on MS Medium, observation growth of callus, and measurement of fresht weigth of callus. The generative explants used were M. oleifera seeds grown on MS medium with 3%, 5%, 7% and 9% sucrose and then they were cultured with photoperiod of 12/12, 14/10, and 16/8 hours (light/dark). The results showed that the best response of growth callus was indicated by the explants cultured on 3% sucrose medium in the photoperiod of 12/12.Keywords: Moringa oleifera; nodule; photoperiod; sucroseÂ

CHARACTERISTICS OF HABITAT, DISTRIBUTION, AND DIVERSITY OF ANOPHELES SPP IN KEMELAK BINDUNG LANGIT VILLAGE, OGAN KOMERING ULU REGENCY, SOUTH SUMATRA Maretasari, Giri; Windusari, Yuanita; Lamin, Syafrina; Hanum, Laila; Septiawati, Dwi
Journal of Environmental Science and Sustainable Development Vol. 2, No. 2
Publisher : UI Scholars Hub

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Malaria is an infectious disease caused by Plasmodium andis transmitted through the bite of a female Anopheles vector. Ogan Komering Ulu (OKU) is a district in South Sumatra that is endemic to malaria. The study aims to determine habitat type, environmental factors that influence larvae development, and distribution of Anopheles larvae. The experiment was conducted from January to February 2019 in the Kelurahan Kemelak Bindung Langit, OKU. Species identification was carried out in the Entomology Laboratory, Baturaja Health Research and Development Center, OKU. Sampling locations were determined based on field observations, through simple purposive sampling. Identification of mosquito larvae which were maintained in the laboratory, showed that they originated from four Anopheles species namely An. vagus, An. barbirostris, An. kochi, and male Anopheles. The dominant habitat (76,89%) was rice fields. The characteristics of larval breeding habitats included water pH of 5-6, water temperature of 280C-320C, light intensity of 756 - 761 mmHg, visual clear water, muddy substrates, and habitat distance with houses of 10 -60 m. The Anopheles type diversity index (H ') was low (0.04-0.36). The larval density was the highest in RT 1 and RT 2 (as many as 2.5 larvae/cauldrons), and the lowest in RW 3 locations (as many as 0.1 larvae/cauldron), which had the same habitat type, namely, rice fields. The highest dominance index (C) was found in male Anopheles (C = 1), and the lowest was detected in An. kochi (C = 0.02) and An. barbirostris (C = 0.01). The pattern of the spread of Anopheles based on the Morishita index was grouped (Id> 1) and uniform (Id <1).

THE DEVELOPMENT OF INVENTORY, MONITORING AND INFORMATION NETWORKS SYSTEM OF FAUNAL DIVERSITY IN SOUTH SUMATRA Yustian, Indra; Setiawan, Arum; Setiawan, Doni; Hanum, Laila; Dahlan, Zulkifli
BIOVALENTIA: Biological Research Journal Vol. 3 No. 2 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Despite high attention and commitment of Indonesia in biodiversity conservation and its habitat, however, loss of biodiversity and habitat deforestation is also still high. Deforestation rate in Indonesia between period of 2000-2012 reach up 6.02 million ha, and approximately onethird of which is in Sumatra Island. As the fulfillment of CBD and Aichi targets, and in line with the mandate of the Law on the Conservation of Natural Resources and Ecosystems and IBSAP (Indonesian Biodiversity Strategy & Action Plan 2015-2020), we try to support the requires data as well as information on the current state of biodiversity. The aims are to develop an inventory system of biodiversity needed to establish baseline data on biodiversity including its conservation status in South Sumatra, develop a biodiversity monitoring system with qualitative parameters that can be used in the determination and monitoring of degradation rates of biodiversity, and the establishment of data management system and information network of fauna biodiversity in South Sumatera so that it can be utilized in monitoring and reporting of biodiversity at regional, national and international level at the international level. We conduct a comprehensive review of methods and techniques of inventory and monitoring of fauna biodiversity, especially those that have been done in the area of South Sumatra Province. We also analysis of data needs and information network of fauna biodiversity in South Sumatra. The results is shows in the web-application database, called the South Sumatran Biodiversity Information Networks, or SSBIN, and could preview at http://ssbin.unsri.ac.id/.Keywords: south sumatra, biodiversity, information, networks, SSBIN.

ROLE OF BAMBOO FOR REVEGETATION OF POST COAL MINING IN SOUTH SUMATERA, INDONESIA Melisyah, Melisyah; Ibrahim, Eddy; Hanum, Laila; Aprianti, Nabila; Wijayanti, Ratih
BIOVALENTIA: Biological Research Journal Vol. 6 No. 2 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Coal mining activities have affected the soil and vegetation conditions on the mined land. Revegetation of ex-mining land is a must to balance environmental functions. The choice of plants must be adapted to the conditions of the ex-mining land so that growth continues. The use of local plants for revegetation is fascinating to study. This study aimed to investigate the soil characteristics of ex-coal mining land and bamboo growth as a plant used for revegetation. Bamboo is collected from around the former coal mining area. Bamboo growth is observed through its branching for 12 weeks of measurement. Soil is analyzed based on its physical and chemical properties. The Schmidt-Ferguson method was used to analyze the climate type in the study area. The results of the soil analysis show that the plants can still grow for revegetation. Of the three types of bamboo observed (Schizostachyum brachycladum kurz, Dendrocalamus asper, and Gigantochloa robusta), Schizostachyum brachycladum kurz is the best growing bamboo in ex-coal mining land with a branch length of 42 cm in 12 weeks of measurement. The climate in the ex-coal mining area studied is a wet type (Q = 0.184). Bamboo is a suitable plant for revegetation, especially in coal mining areas in South Sumatra, Indonesia.

EXTRACTION AND PURITY DNA OF Culex spp MOSQUITO IN KEMELAK VILLAGE, BINDUNG LANGIT, OGAN KOMERING ULU Nuriessa Aputri, Farah; Hanum, Laila; Safitri, Ayu; Windusari, Yuanita
BIOVALENTIA: Biological Research Journal Vol. 7 No. 2 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Culex spp are mosquito vectors that have a very wide distribution capability and are carriers of pathogens that can interfere with human and animal health. The wide distribution makes Culex spp a dangerous threat. DNA extraction is one of the important steps in obtaining genetic information and genetic analysis. Good quality DNA is used for activities such as the use of molecular markers, genome library creation, and sequencing. This study aims to determine the quality, concentration and purity of Culex spp mosquito DNA in Kemelak Bindung Langit Village, OKU Regency. It is hoped that the sample can be used for further research analysis on Mitochondria D-Loop Sequences in Culex spp mosquitoes. Quantitative measurement of DNA in the form of concentration and purity of DNA using Nanodrop Thermo cycle while qualitative DNA using electrophoresis technique. The results of the isolation of the mosquito genome DNA, obtained clear DNA bands without any degradation (smear) and the concentration results for the four samples ranged from 10-100 ng/ÂµL and the DNA purity was good, ranging from 1.8 to 2.00.

THE EFFECT OF CULTURE MEDIA TYPE AND PLANT GROWTH REGULATORS ON CALLUS INDUCTION OF OIL PALM (Elaeis guineensis Jacq) PISIFERA TYPE Sulaksono, Gogoh; Hasmeda, Mery; Hanum, Laila; Wendra, Fahmi; Santika, Baitha; Asmono, Dwi
BIOVALENTIA: Biological Research Journal Vol. 7 No. 2 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Oil palm has a high economic value since it is one of the highest vegetable oil-producing plants compared to other oil-producing plants. The provision of good planting material for oil palm plantations is one of the determining factors to increase productivity. The parent Pisifera type of oil palm is important to develop the high-yielding oil palm Tenera varieties. The provision of oil palm material could be done through generative system with seeds and also could be done through vegetative approaches using tissue culture techniques. It aims to get plants that are genetically the same as their parents (true to type). The purpose of this study was to see the effect of different type of tissue culture media and plant growth regulators (PGRâ€™s) on the callus induction stage of Pisifera type oil palm. The results show that the treatment using Murasige and Skoog (MS) culture media is able to give a better effect when compared to the use of Eeuwens and Blake (Y3) basic media. While the use of PGR treatment (H6 = NAA 6 mg/l + 2.4-D 0.5 mg/l) showed a callus growth percentage of 76%, which was better than (H1 = Kin 0.1 mg/l + 2.4-D 100 mg/l) by 42%.

Analisis Vegetasi di Kawasan Terbuka Hijau Industri Gasing Siti Indah Oktaviani; Laila Hanum; Zaidan P Negara
Jurnal Penelitian Sains Vol 19, No 3 (2017)
Publisher : Faculty of Mathtmatics and Natural Sciences

Penelitian bertujuan untuk mengetahui keanekaragaman vegetasi di kawasan terbuka hijau industri Gasing. Penelitian dilakukan dari bulan April - Juli 2017. Metode analisis mengunakan metode kuadrat den-gan 2 plot pada lokasi yang berbeda dengan tiga kali pengulangan, analisa data kuantitatif dilakukan terhadap nilai kerapatan, dominasi, frekuensi, nilai penting dan indeks keanekaragaman masing-masing jenis tumbuhan. Hasil penelitian menunjukan bahwa vegetasi terdiri dari 16 suku dan 19 jenis, didominasi oleh Pteridophyta dan Spermatophyta. nilai keanekaragaman vegetasi sebesar (2,24) dan nilai keseragaman 0,30. Semakin ting-gi jumlah jenis maka semakin tinggi indeks keanekaragaman suatu jenis. Karakteristik yang demikian disebab-kan oleh pengaruh lingkungan yang dimiliki seperti kandungan bahan organik dan pH rendah serta faktor yang berasal campur tangan manusia.

Analisis Polimorfisme Padi Varietas Lokal Sumatera Selatan Berdasarkan Pendekatan PCR-RAPD Fikri Adriansyah; Laila Hanum; Muharni Muharni; Yuanita Windusari
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol. 7 No. 1 (2018): JLSO
Publisher : Research Center for Sub-optimal Lands (PUR-PLSO), Universitas Sriwijaya

Adriansyah et al, 2018. Polymorphism Analysis of Local Varieties of South Sumatra Rice Based on PCR-RAPD. JLSO 7(1):50-58.The existence of rice germplasm is threatened because of the introduction of various high-yielding varieties. This study aimed to analyze rice kinship and conservation of local varieties of South Sumatra rice. This study was conducted in August 2015 to December 2015. DNA isolation using the Promega Wizard Purification Systems KIT. This study uses OPA-9 primer, OPA-10, OPA-13, OPA-16. All primers can amplify DNA with optimal quality. OPA-9 primers produced 5 DNA bands, OPA-10 primers produced 9 DNA bands, OPA-13 primers produced 12 DNA and Primary OPA-16 bands producing 9 ppita DNA.

Co-Authors Aditya K. Karim Aditya K. Karim Adriansyah, Fikri Affandi, Azhar Kholiq Affrita, Tia Maya Afnur Imsya Aldes Lesbani Ali Djamhuri Amin Ali Mubarok Amin Rejo Andika Puspita Dewi Artanti, Widyana Beta Arum Setiawan Arum Setiawan Arum Setiawan Arum Setiawan Aryani, Robeah Ayu Nursafitri Ayu Safitri Chatrin Yohana Simamora Dedek Kurniawan Deni Julius Destia Maharani Destri, Erin Dewi Lisandra Doni Setiawan Dwi Asmono Dwi Septiawati, Dwi Eddy Ibrahim Edward Saleh Eka Haryati Yuliany Elisa Nurnawati Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina, Elyani Ami Endro Sastro cahyono Enggar Patriono Ervina Indrayani Ervina Indrayani Erwin Nofyan Evi Yuliza Evi Yuliza Evi Yuliza, Evi Fahira, Aulia Fidella Oktariana Fikri Adriansyah Fikri Adriansyah Fikri Adriansyah Fitri Maya Puspita Fitri Maya Puspita Fitri Maya Puspita Fitri Maya Puspita Hary Widjajanti hasana, nurdiah Helena Valenta Br Kemit Hernanda, Eggyana Deri Hikayati I Gusti Wayan Murjana Yasa Indra Yustian Irmawan Farindra Irmeilyana Juswardi Juswardi, Juswardi Lestari, Astri Indah Lestari, Margareta Sri Maretasari, Giri Margareta Sri Lestari Margareta Sri Lestari, Margareta Sri Maya Puspita, Fitri Melisyah, Melisyah Melwita, Elda Mery Hasmeda Muharni Muharni Mustafa Kamal Nabila Aprianti Negara, Zaidan Panji Ngudiantoro . Nina Tanzerina Nita Aminasih Nita Aminasih Nur Arifah Nuriessa Aputri, Farah Nursela, Dewi Parwanti, Sri Permata, Ending Putra Bahtera Jaya Bangun Putra Bahtera Jaya Bangun R. Rugayah Rahmat Pratama Rahmat Pratama Rahmat Pratama, Rahmat Ranti Sawitri Ratih Wijayanti, Ratih Reza Andeka Rina S. Kasiamdari Rina S. Kasiamdari Rina Sri Kasiamdari Rizka Aniza Robeah Aryani Rosmania, Rosmania Rugayah Rugayah Rupiah Rupiah Rupiah Rusdi, Muhammad Salsabeela S. Santosa Salni Santika, Baitha Santosa . Santosa Santosa Sarno Sarno Sarno Sarno Sarno Sarno Sarno Sarno Singgih Tri Wardana Singgih Tri Wardana Sisca Octarina Sisca Puspita Sepriliani Siti Indah Oktaviani Siti Indah Oktaviani Siti Suzlin Supadi Suheyanto Suheryanto Sulaksono, Gogoh Syafrina Lamin, Syafrina Tri Handayani Venty Warda Elmaida Rusdi Wendra, Fahmi Wijaya, Alfan Windusari, Yuanita Yolanda Fitria Yuanita Windusari Yuanita Windusari Yuanita Windusari Yuanita Windusari Yuanita Windusari Yuanita Windusari Yulia Anisawati Yuliany, Eka Haryati Zahrial Effendi Zaidan P Negara Zulkifli Dahlan Zulkifli Dahlan Zulkifli Dahlan

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